A benchmarking of long-read RNA sequencing methods and analysis tools The Long-read RNA-Seq Genome Annotation Assessment Project (LRGASP) consortium, an initiative to systematically evaluate methods for transcript identification and quantification,…
PacBio HiFi long-read sequencing technology has made huge strides in helping us understand tumor biology, from the ability to call more variant classes in tumor genomes, to understanding the way…
Highly-accurate HiFi reads produced by PacBio long-read sequencers –such as the Sequel IIe and Revio systems– enable researchers to access entirely new levels of contiguity, completeness, and accuracy when assembling…
Since the inception of next generation sequencing (NGS) more than a decade ago, short-read sequencing accuracy has seen only marginal improvement. Having achieved a level of precision thought to be…
As more researchers embrace the benefits of PacBio long-read sequencing technology, an expanding community of analysis tool developers has taken shape. Thanks to this growing excitement around PacBio capabilities there…
Cellular cartography has been just as important for biologists to navigate the complex topography of living systems as was the mapping of the earth’s landscapes for explorers hundreds of years…
Scientific breakthroughs are rarely achieved in isolation. They require collaboration, cutting-edge techniques, and a platform where scientists can come together to exchange knowledge and explore groundbreaking tools. And that’s exactly…
The new Revio long-read sequencing system, with its first customer shipment in March 2023, has changed the game in genomics. It enables researchers to access an entirely new echelon of…
If you have ever clicked around the PacBio website or attended a workshop or webinar discussing PacBio long-read sequencing technology, then you have probably seen or heard about SMRT (Single…
Delve deeper into the importance of capturing diversity with population-specific reference pangenomes. The movement towards the development of pangenomes is gaining steam, bringing the reality of truly personalized medicine closer….
The Revio system was built with the belief that game-changing technology should be simple to use. For a technology to be truly revolutionary it should deliver superior performance in conjunction…
Fresh off the special Nature issue last month on the first human pangenome reference (featured in a previous blog), the first regional human pangenome reference study, entitled A Pangenome Reference…
When developing effective personalized immunotherapies, such as cancer vaccines, a pivotal factor lies in uncovering tumor neoantigens that can serve as crucial therapeutic targets. Traditionally, neoantigen discovery heavily relied on…
Large-scale population sequencing programs have a number of important research goals and outcomes. These include: Generating a comprehensive catalogue of genetic variation to reflect the genetic diversity in a target…
This article explores sequencing coverage fundamentals. Uncover key concepts and discover how highly accurate long-read sequencing provides a comprehensive view of the genome, at any coverage level. What is sequencing…
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