Targeted sequencing
Enrich your understanding with accurate detection of all variant types, even in the hardest-to-reach regions of the genome
Targeted sequencing solutions
PacBio targeted sequencing applications include HLA sequencing as well as applications in human biomedical research, plant and animal science, and microbial and infectious disease.
Human Genomics
Cost-effectively explore the full variation of the regions of the genome that matter to you.
HLA SEQUENCING
Overcome gene-family complexity and extreme polymorphism to identify true HLA allelic diversity.
PLANT + ANIMAL SCIENCES
Gain a comprehensive view of specific genomic regions of interest, regardless of genome size or complexity.
MICROBIAL + INFECTIOUS DISEASE RESEARCH
Resolve viral populations to better understand evolution, quasispecies dynamics, drug-resistance, and immune escape
Targeted sequencing in action
Video
Advancing pharmacogenomics research
HiFi sequencing enables comprehensive interrogation of pharmacogenomics genes such as single-gene assays or large panels.
NEW PRODUCT: HIFI TARGET ENRICHMENT WITH TWIST PROBES
Learn how you can get all the benefits of long and accurate HiFi reads at scale.
Long reads elucidate HIV envelope variants
Caskey and coworkers use targeted SMRT sequencing to determine that pre-existing variants in the HIV envelope may confer resistance to…
Identifying key players in host-microbiome interactions
HiFi sequencing of extended 16S amplicons has enabled the identification of metagenome community members at higher taxonomic…
EXPLORE
Did you know we have a comprehensive library of articles, reports, papers, and videos related to targeted sequencing?
Resources
Application Notes
- Targeted HiFi sequencing for congenital adrenal hyperplasia
- Targeted HiFi sequencing for Thalassemia
- HiFi amplicon sequencing for pharmacogenetics: CYP2D6
Application Briefs
Protocols
Pick the workflow right for your lab
HiFi Target Enrichment
Access more of the genome with an end-to-end solution optimized for long reads.
Target large or small gene panels with an integrated and scalable workflow using custom DNA probes from Twist Bioscience. HiFi target enrichment can span regions of the genome inaccessible to other technologies and comprehensively calls both small variants and structural variants. Tiled HiFi reads enable long-range phasing and accurate haplotype resolution.
Learn more about Twist and plan your project with this app brief.
HiFi amplicon sequencing
Enjoy a simplified workflow with one primer pair and one amplicon pool, easy assay optimization, long-range phasing, and simplified data analysis. Target medically relevant, individual genes or small gene panels using a PCR-based approach. If you can amplify it, HiFi sequencing can span your amplicon in a single read, giving unambiguous haplotype resolution through direct phasing. HiFi amplicon sequencing allows you to replace multiple assays with one fast, easy, scalable, and economical workflow.
Plan your project with this resource and learn more about our barcoding products.
Targeted sequencing workflow at a glance
Easy-to-use, high-throughput sequencing for the accuracy you need at an affordable cost
Straightforward library prep
Go from sample to sequencer in a single afternoon
Complete + accurate sequencing
Obtain accurate, unbiased, and long reads
Directly detect epigenetic modifications
Universal file types
Analyze data using any of the common genome assembly and analysis tools
YOUR MOMENT IS WAITING
Long reads at a lower cost and higher throughput. Short reads with unmatched accuracy. From the fullest picture to the finest detail, your moment of discovery is waiting.