Targeted sequencing solutions
PacBio targeted sequencing applications include HLA sequencing as well as applications in human biomedical research, plant and animal science, and microbial and infectious disease.
HUMAN BIOMEDICAL RESEARCH
Cost-effectively explore the full variation of the regions of the genome that matter to you.
Overcome gene-family complexity and extreme polymorphism to identify true HLA allelic diversity.
PLANT + ANIMAL SCIENCES
Gain a comprehensive view of specific genomic regions of interest, regardless of genome size or complexity.
Introduction to targeted sequencing
If you are using PCR, hybridization-capture, or amplification-free enrichment, HiFi sequencing allows you to discover and detect the full breadth of variants in targeted regions of the genome and transcriptome.
PacBio targeted sequencing:
Is more complete than other technologies
Confidently capture more accurate genomic information, even in regions with complex pseudogenes or low complexity regions such as repeat expansions.
Includes the full spectrum of variants
Uncover biology that other sequencing methods can miss by detecting everything from SNPs to structural variations while ensuring accurate phasing throughout.
Enables a variety of enrichment methods
HiFi sequencing allows you to use familiar target enrichment methods like amplicon sequencing or hybridization capture.
Targeted sequencing in action
NEW PRODUCT: HIFI TARGET ENRICHMENT WITH TWIST PROBES
Learn how you can get all the benefits of long and accurate HiFi reads at scale.
Long reads elucidate HIV envelope variants
Caskey and coworkers use targeted SMRT sequencing to determine that pre-existing variants in the HIV envelope may confer resistance to…
Identifying key players in host-microbiome interactions
HiFi sequencing of extended 16S amplicons has enabled the identification of metagenome community members at higher taxonomic…
SHAPING THE FUTURE
Using an impressive array of novel and cutting-edge technologies, SMRT sequencing enabled scientists to find recombination in a gene associated with Alzheimer’s disease.
Benefits of targeted
See the whole picture with long read lengths
Long and accurate HiFi reads allow comprehensive variant detection from single nucleotide polymorphism to insertions and deletions to structural variants.
With HiFi sequencing you can simplify analysis by reducing or eliminating the need for assembly, depending on the length of your region of interest.
Identify which variants are allelic with accurate long-range phasing
Long, accurate reads ensure you have the highest confidence in phasing haplotypes and compound variant identification, even when variants are separated by tens of kilobases.
Focus your sequencing on transcripts that matter while achieving the same benefits that SMRT sequencing delivers for complete transcriptome sequencing.
You can phase variants, profile isoforms, and obtain 5’ UTR sequences with long and accurate reads.
Amplify target sequences
When sequencing amplicons, SMRT sequencing provides a fast, straightforward workflow with low DNA input requirements, phasing capability, and the chance to iterate quickly.
With SMRT sequencing’s long read lengths, you can sequence full length amplicons.
Enrich Multiple Targets with Probe Capture
For multiple genes, hybridization capture can enrich many targets at once. Target single regions of interest or resolve an entire gene or gene family with a tiled probe design.
Probe capture can enrich DNA or cDNA from humans, plants and animals, or viruses.
Targeted sequencing workflow at a glance
Easy-to-use, high-throughput sequencing for the accuracy you need at an affordable cost
Straightforward library prep
Go from sample to sequencer in a single afternoon
Complete + accurate sequencing
Obtain accurate, unbiased, and long reads
Directly detect epigenetic modifications
Universal file types
Analyze data using any of the common genome assembly and analysis tools