Targeted sequencing

Enrich your understanding with accurate detection of all variant types, even in the hardest-to-reach regions of the genome

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Targeted sequencing solutions

PacBio targeted sequencing applications include HLA sequencing as well as applications in human biomedical research, plant and animal science, and microbial and infectious disease.

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Human Genomics

Cost-effectively explore the full variation of the regions of the genome that matter to you.

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Overcome gene-family complexity and extreme polymorphism to identify true HLA allelic diversity.

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Gain a comprehensive view of specific genomic regions of interest, regardless of genome size or complexity.

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Resolve viral populations to better understand evolution, quasispecies dynamics, drug-resistance, and immune escape

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Targeted sequencing in action

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Learn how you can get all the benefits of long and accurate HiFi reads at scale.

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Long reads elucidate HIV envelope variants

Caskey and coworkers use targeted SMRT sequencing to determine that pre-existing variants in the HIV envelope may confer resistance to…

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Identifying key players in host-microbiome interactions

HiFi sequencing of extended 16S amplicons has enabled the identification of metagenome community members at higher taxonomic…

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Did you know we have a comprehensive library of articles, reports, papers, and videos related to targeted sequencing?

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Pick the workflow right for your lab

HiFi Target Enrichment

Combine Twist Bioscience target enrichment with PacBio long and accurate HiFi reads to efficiently sequence your priority genomic regions at scale. Sequence enriched regions with a protocol optimized for HiFi reads to get comprehensive detection of single nucleotide variants, structural variants, and indels with haplotype resolution. HiFi target enrichment can deliver accurate alleles for complex gene families such as immune genes (e.g., HLA) and pharmacogenes (e.g., CYP2D6).

HiFi target enrichment enables you to sequence at scale with ready-made Twist Alliance Panels or custom designs. Learn more about Twist and plan your project with this app brief.

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HiFi amplicon sequencing

Enjoy a simplified workflow with one primer pair and one amplicon pool, easy assay optimization, long-range phasing, and simplified data analysis. Target medically relevant, individual genes or small gene panels using a PCR-based approach. If you can amplify it, HiFi sequencing can span your amplicon in a single read, giving unambiguous haplotype resolution through direct phasing. HiFi amplicon sequencing allows you to replace multiple assays with one fast, easy, scalable, and economical workflow.

Plan your project with this resource and learn more about our barcoding products.

Targeted sequencing workflow at a glance

Easy-to-use, high-throughput sequencing for the accuracy you need at an affordable cost

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Straightforward library prep

Go from sample to sequencer in a single afternoon

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Complete + accurate sequencing

Obtain accurate, unbiased, and long reads

Directly detect epigenetic modifications

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Universal file types

Analyze data using any of the common genome assembly and analysis tools

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Long reads at a lower cost and higher throughput. Short reads with near-perfect accuracy. From the fullest picture to the finest detail, your moment of discovery is waiting.

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