Sequencing 101

Follow along to learn more about our technology, reveal the impacts of long-read sequencing, and uncover tips on how to get started.

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Sequencing 101: long-read sequencing

Long-read sequencing technologies are quickly becoming the new gold standard in genomics research, and HiFi sequencing is at the forefront. This article provides an introductory look at what long-read sequencing is, and explores topics the advantages, applications of using long-read sequencing in your research.

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Sequencing 101: Structural variation

Over the last 50 years, many monumental achievements in genetics and biology have unfolded before our eyes. From the invention of DNA sequencing technologies to the completion of the human…

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Sequencing 101: Tandem repeats

In the field of genetics, the concept of tandem repeats has been both scientifically fascinating, experimentally challenging, and motivating for technology development. As DNA sequencing technologies and analysis tools have…

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Sequencing 101: SBB sequencing

Since the inception of next generation sequencing (NGS) more than a decade ago, short-read sequencing accuracy has seen only marginal improvement. Having achieved a level of precision thought to be…

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Sequencing 101: The SMRT Cell in PacBio long-read sequencing

If you have ever clicked around the PacBio website or attended a workshop or webinar discussing PacBio long-read sequencing technology, then you have probably seen or heard about SMRT (Single…

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Sequencing 101: Sequencing coverage

This article explores sequencing coverage fundamentals. Uncover key concepts and discover how highly accurate long-read sequencing provides a comprehensive view of the genome, at any coverage level. What is sequencing…

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Sequencing 101: How does whole genome sequencing help us understand rare diseases?

  Updated on March 2, 2023 Rare diseases are defined as diseases that affect a small number of people — fewer than 1 in 2,000 in the European Union and…

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Sequencing 101: DNA extraction — tips, kits, and protocols

  If you are like most of us at PacBio, you likely learned how to extract DNA in a high school or college biology class, or maybe even in your…

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Genomes vs. genNNNes: the difference between contigs and scaffolds in genome assemblies

  Updated on February 24, 2023. Originally published September, 2016. In recent interactions with the scientific community, we’ve seen a growing number of questions around scaffolding genome assemblies. We thought…

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Sequencing 101: ploidy, haplotypes, and phasing — how to get more from your sequencing data

  Geneticists often point out that a human does not have “a” genome but rather two genomes: one inherited from the mother and another from the father. The number of…

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