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Training

Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.

April 20, 2022

Technical overview – Adeno-associated virus (AAV) library preparation using SMRTbell Prep Kit 3.0

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of adeno-associated virus (AAV) library preparation for viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing AAV samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.
April 20, 2022

Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation describes sample preparation details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II and Sequel IIe systems. Options for multiplexing WGS samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics are also presented.
April 20, 2022

Technical overview – Iso-Seq library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of Iso-Seq library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing Iso-Seq samples using barcoded cDNA primers or SMRTbell barcoded adapters are discussed. Sequencing preparation workflow details, example performance data and Iso-Seq data analysis recommendations are also presented.
April 20, 2022

Technical overview – Multiplexed amplicon library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0) in conjunction with barcoded primers, SMRTbell barcoded adapters or barcoded M13 primers. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations are also presented. Additional guidance for preparing multiplexed amplicon libraries for full-length 16S amplicon sequencing and Human Leukocyte Antigen (HLA) sequencing is also summarized.
April 20, 2022

Technical overview – Multiplexed SARS-CoV-2 library preparation for full-viral genome sequencing using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of multiplexed library preparation for full-viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0) and the HiFiViral SARS-CoV-2 Kit for viral genome enrichment. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.
April 20, 2022

HiFi sequencing and software v11.0 release – Technical overview for Sequel II & Sequel IIe system users

  • Tutorial
  • Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of key features & benefits of the new HiFi sequencing and software v11.0 release for Sequel II and Sequel IIe systems. Topics covered include new consumables for SMRTbell library preparation & sequencing preparation (ABC) and SMRTbell library construction workflow updates for whole genome sequencing, viral sequencing, Iso-Seq analysis and multiplexed amplicon sequencing applications using the new SMRTbell prep kit 3.0. New instrument control software capabilities such as automated 5mC detection as well as other updated and new SMRT Link features in Sample Setup, Run Design, Run QC and SMRT Analysis are also discussed.
February 5, 2021

No-Amp Targeted Sequencing Library Preparation and Data Analysis Overview

  • Tutorial
  • Sequel II System, Sequel IIe System, Sequel System
  • Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of the No-Amp Targeted Sequencing workflow and summarizes No-Amp library preparation & sequencing workflow details, No-Amp example performance data, No-Amp assay design procedures for analyzing custom targets, and No-Amp data analysis tools.
February 5, 2021

Single-Cell Iso-Seq Library Preparation Using SMRTbell Express TPK 2.0

  • Tutorial
  • Sequel II System, Sequel IIe System, Sequel System
  • Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation describes sample library preparation & sequencing workflow details for Single-Cell Iso-Seq applications and example performance data.
February 5, 2021

Ultra-Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0

  • Tutorial
  • Sequel II System, Sequel IIe System
  • Binding, Sample Preparation, Sequencing, Template Preparation

This technical training presentation provides an overview of the ultra-low DNA input workflow and summarizes experimental design considerations, ultra-low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and ultra-low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
March 18, 2020

Tutorial: Bioinformatics Lunch & Learn – HiFi Assembly

  • Tutorial
  • Sequel II System
  • Analysis

The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes. The long length and high accuracy of HiFi reads makes them the ideal starting point for many applications, and one area of major interest is genome assembly. HiFi assembly is faster, cheaper, more accurate, and easier to phase than standard long-read assembly. In this webcast, you will learn: The difference between long reads and HiFi reads; the strengths of HiFi read assembly for human, plant, animal, and insect genomes; step-by-step instruction on how to assemble HiFi reads using the publicly available FALCON package (https://github.com/PacificBiosciences/pb-assembly)
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