Training
Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.
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HiFi Library Preparation Using SMRTbell Express TPK 2.0
Sequel II System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of HiFi sequencing features & applications and summarizes sample preparation details for constructing HiFi libraries using the SMRTbell Express Template Prep Kit 2.0. 'Best Practices' overviews for sample library preparation and sequencing are also presented for whole genome sequencing for de novo assembly and variant detection applications using HiFi reads.
No-Amp Targeted Sequencing Library Preparation and Data Analysis Overview
Sequel II System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of the No-Amp Targeted Sequencing workflow and summarizes No-Amp library preparation & sequencing workflow details, No-Amp example performance data, No-Amp assay design procedures for analyzing custom targets, and No-Amp data analysis tools. Links to technical documentation and applications support resources for No-Amp Targeted Sequencing are also presented.
Single-Cell Iso-Seq Library Preparation Using SMRTbell Express TPK 2.0
Sequel II System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for Single-Cell Iso-Seq applications and example performance data (Sequel II System v8.0).
Full-Length 16S Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for full-length 16S applications and example performance data (Sequel II System v8.0 / Chemistry 2.0).
Multiplexed Library Preparation For Structural Variation Detection Using SMRTbell Express Template Prep Kit 2.0
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes multiplexed sample library preparation & sequencing workflow details for structural variation detection applications and multiplexed SV library example performance data (Sequel II System v8.0 / Chemistry 2.0). General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Multiplexed Microbial Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes multiplexed microbial sample preparation & sequencing workflow details, multiplexed microbial data analysis workflow details, and multiplexed microbial library example performance data (Sequel System v6.0 / Chemistry v3.0 and Sequel II System v8.0 / Chemistry 2.0). General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Iso-Seq Express Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of Iso-Seq Express library sample preparation using the SMRTbell Express Template Preparation Kit 2.0. Sequencing workflow details, Iso-Seq Express library sequencing performance example data (Sequel System v6.0 / Chemistry 3.0 and Sequel II System v8.0 / Chemistry 2.0), and Iso-Seq application best practices are also presented.
Tutorial: Bioinformatics Lunch & Learn – HiFi Assembly
Sequel II System
Analysis
Description +
The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes. The long length and high accuracy of HiFi reads makes them the ideal starting point for many applications, and one area of major interest is genome assembly. HiFi assembly is faster, cheaper, more accurate, and easier to phase than standard long-read assembly. In this webcast, you will learn: The difference between long reads and HiFi reads; the strengths of HiFi read assembly for human, plant, animal, and insect genomes; step-by-step instruction on how to assemble HiFi reads using the publicly available FALCON package (https://github.com/PacificBiosciences/pb-assembly)
Sequel II System v8.0 & SMRT Link v8.0 Technical Overview
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of key features & benefits of the Sequel II System v8.0 and SMRT Link v8.0. 'Best Practices' overviews for sample library preparation and sequencing are also presented for whole genome sequencing for de novo assembly, whole genome sequencing for structural variation detection, variant detection using whole genome sequencing with HiFi reads, No-Amp targeted sequencing, long-read RNA sequencing, and metagenomic sequencing of complex populations.
Metagenomics Shotgun Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for metagenomics shotgun gene profiling & assembly applications and example performance data (Sequel II System v8.0 / Chemistry 2.0).
Tutorial: Bioinformatics Lunch & Learn – Better assemblies of bacterial genomes and plasmids with the new microbial assembly pipeline in SMRT Link v8.0
Sequel II System
Analysis
Description +
Microbial Assembly is our latest pipeline, specifically designed to assemble bacterial genomes (between 2 and 10 Mb) and plasmids. This pipeline includes the implementation of a new, circular-aware read alignment tool (Raptor), among other algorithmic improvements, which will be covered in this webinar. The topics covered include, staged assembly of bacterial chromosomes and plasmids, implementation of Raptor, a circular-aware read aligner, himeric read detection, origin of replication orientation, troubleshooting and more.
Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of the low DNA input workflow and summarizes experimental design considerations, low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and low DNA input library example performance data (Sequel System 6.0 / Chemistry 3.0 and Sequel II System v8.0 / Chemistry 2.0). General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Tutorial: Bioinformatics Lunch & Learn – Command line analysis with Cromwell in PacBio SMRT Link v8.0
Sequel II System
Analysis
Description +
In this webinar, Matthew Seetin a PacBio Bioinformatics Field Application Scientist, presents one of the biggest engineering changes in SMRT Link v8.0 - the migration from pbsmrtpipe to Cromwell. With this change, a number of new features particularly suited for pipeline engineers and production facilities are now available for use. Learn from our BFX FAS to see how you can best take advantage of this new, versatile and scalable platform. Topics covered include: What is Cromwell and how does it help you? New features including data caching and job restart, and guidance for building custom workflows combining PacBio & 3rd party tools.
Multiplexed Amplicon Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of multiplexed amplicon library sample preparation using the SMRTbell Express Template Preparation Kit 2.0. Sequencing workflow details, multiplexed amplicon library sequencing performance example data, and targeted amplicon sequencing application best practices are also presented.
Best Practices for Working with PacBio Tools (SMRT Link & Developmental) on the Command Line
Sequel II System, Sequel System
Analysis
Description +
This webinar, presented by Roberto Lleras, provides the best practices for working with PacBio tools (SMRT Link and Developmental) on the command line. Please access this file for step-by-step instructions for installing SMRT Analysis tools and pbbioconda in order to analyze SMRT Sequencing data on the command line: https://www.pacb.com/wp-content/uploads/Bioinformatics-Webinar-Session-1-Getting-Started.pdf