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Training

Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.

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Multiplexed Microbial Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation describes multiplexed microbial HiFi library sample preparation & sequencing workflow details, multiplexed microbial HiFi data analysis workflow details, and multiplexed microbial HiFi library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.

Multiplexed Library Preparation for Full-Viral Genome Sequencing Using HiFiViral SARS-CoV-2 Kit

Tutorial
Sequel II System, Sequel IIe System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of multiplexed library preparation for full-viral genome sequencing using the HiFiViral SARS-CoV-2 Kit for viral genome enrichment. SMRT sequencing preparation details for Sequel II and IIe Systems, HiFiViral sequencing data analysis workflows using SMRT Link, and HiFiViral SARS-CoV-2 example performance data are also discussed.

HiFi Microbial Assembly, HiFiViral SARS-CoV-2 Kit and SMRT Link v10.2 Release – Technical Overview for Sequel II System & Sequel IIe System Users

Tutorial
Sequel II System, Sequel IIe System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of key features & benefits of the new HiFi Microbial Assembly, HiFiViral SARS-CoV-2 Kit and SMRT Link v10.2 Release. Topics covered include new Sequel II and IIe System consumables, and microbial whole genome sequencing (WGS) and & full-viral genome sequencing sample preparation workflow updates. In addition, new SMRT Link v10.2 features and analysis application updates for HiFiViral SARS-CoV-2 viral sequencing data and HiFi Microbial Assembly whole genome sequencing data are discussed.

HiFi Library Preparation Using SMRTbell Express TPK 2.0 for De Novo Assembly and Variant Detection

Tutorial
Sequel II System, Sequel IIe System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation describes sample preparation details for constructing HiFi libraries using SMRTbell Express Template Prep Kit 2.0 for de novo assembly and variant detection applications. Example sequencing performance data for large insert HiFi libraries are also summarized.

Ultra-Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of the ultra-low DNA input workflow and summarizes experimental design considerations, ultra-low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and ultra-low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.

Iso-Seq Express Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of Iso-Seq Express library sample preparation using SMRTbell Express Template Preparation Kit 2.0. Sequencing workflow details, Iso-Seq Express library sequencing performance example data and Iso-Seq application best practices are also presented.

Multiplexed Amplicon Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell Express Template Preparation Kit 2.0 in conjunction with Barcoded Overhang Adapters (BOA) / Barcoded Universal Primers (BUP) / Barcoded M13 Primers. Sequencing workflow details, multiplexed amplicon library sequencing performance example data, and targeted amplicon sequencing application best practices are also presented. Additional guidance for preparing multiplexed amplicon libraries for Human Leukocyte Antigen (HLA) sequencing is also summarized.

No-Amp Targeted Sequencing Library Preparation and Data Analysis Overview

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of the No-Amp Targeted Sequencing workflow and summarizes No-Amp library preparation & sequencing workflow details, No-Amp example performance data, No-Amp assay design procedures for analyzing custom targets, and No-Amp data analysis tools.

Full-Length 16S Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation describes sample library preparation & sequencing workflow details for full-length 16S applications and example performance data.

Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation provides an overview of the low DNA input workflow and summarizes experimental design considerations, low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.

Single-Cell Iso-Seq Library Preparation Using SMRTbell Express TPK 2.0

Tutorial
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation

Description +

This technical training presentation describes sample library preparation & sequencing workflow details for Single-Cell Iso-Seq applications and example performance data.

Tutorial: Bioinformatics Lunch & Learn – HiFi Assembly

Tutorial
Sequel II System
Analysis

Description +

The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes. The long length and high accuracy of HiFi reads makes them the ideal starting point for many applications, and one area of major interest is genome assembly. HiFi assembly is faster, cheaper, more accurate, and easier to phase than standard long-read assembly. In this webcast, you will learn: The difference between long reads and HiFi reads; the strengths of HiFi read assembly for human, plant, animal, and insect genomes; step-by-step instruction on how to assemble HiFi reads using the publicly available FALCON package (https://github.com/PacificBiosciences/pb-assembly)

Tutorial: Bioinformatics Lunch & Learn – Command line analysis with Cromwell in PacBio SMRT Link v8.0

Tutorial
Sequel II System
Analysis

Description +

In this webinar, Matthew Seetin a PacBio Bioinformatics Field Application Scientist, presents one of the biggest engineering changes in SMRT Link v8.0 - the migration from pbsmrtpipe to Cromwell. With this change, a number of new features particularly suited for pipeline engineers and production facilities are now available for use. Learn from our BFX FAS to see how you can best take advantage of this new, versatile and scalable platform. Topics covered include: What is Cromwell and how does it help you? New features including data caching and job restart, and guidance for building custom workflows combining PacBio & 3rd party tools.

Best Practices for Working with PacBio Tools (SMRT Link & Developmental) on the Command Line

Tutorial
Sequel II System, Sequel System
Analysis

Description +

This webinar, presented by Roberto Lleras, provides the best practices for working with PacBio tools (SMRT Link and Developmental) on the command line. Please access this file for step-by-step instructions for installing SMRT Analysis tools and pbbioconda in order to analyze SMRT Sequencing data on the command line: https://www.pacb.com/wp-content/uploads/Bioinformatics-Webinar-Session-1-Getting-Started.pdf