April 20, 2022
Technical overview – Adeno-associated virus (AAV) library preparation using SMRTbell Prep Kit 3.0
- Tutorial
- Sequel II System, Sequel II System:Sequel IIe System, Sequel IIe System
- Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
This technical training presentation provides an overview of adeno-associated virus (AAV) library preparation for viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing AAV samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.