Training
Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.
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HiFi Sequencing and Software v10.1 Release Technical Overview for Sequel II System and Sequel IIe System Users
Sequel II System, Sequel IIe System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of key features & benefits of the HiFi Sequencing and Software v10.1 Release. Topics covered include Sequel II and IIe System Instrument Control Software v10.1 updates, Sequel II and IIe System consumables and HiFi sample preparation workflow updates, and new SMRT Link v10.1 features.
HiFi Library Preparation Using SMRTbell Express TPK 2.0 for De Novo Assembly and Variant Detection
Sequel II System, Sequel IIe System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample preparation details for constructing HiFi libraries using SMRTbell Express Template Prep Kit 2.0 for de novo assembly and variant detection applications. Example sequencing performance data for large insert HiFi libraries are also summarized.
PacBio HiFiViral Workflow Overview: Multiplexed Amplicon Library Preparation for Full-Viral Genome Sequencing of SARS-CoV-2
Sequel II System, Sequel IIe System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of PacBio's HiFiViral workflow for SARS-CoV-2 full-viral genome sequencing and summarizes multiplexed amplicon library preparation & sequencing workflow details.
Ultra-Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of the ultra-low DNA input workflow and summarizes experimental design considerations, ultra-low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and ultra-low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Iso-Seq Express Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of Iso-Seq Express library sample preparation using SMRTbell Express Template Preparation Kit 2.0. Sequencing workflow details, Iso-Seq Express library sequencing performance example data and Iso-Seq application best practices are also presented.
Multiplexed Amplicon Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell Express Template Preparation Kit 2.0 in conjunction with Barcoded Overhang Adapters (BOA) / Barcoded Universal Primers (BUP) / Barcoded M13 Primers. Sequencing workflow details, multiplexed amplicon library sequencing performance example data, and targeted amplicon sequencing application best practices are also presented. Additional guidance for preparing multiplexed amplicon libraries for Human Leukocyte Antigen (HLA) sequencing is also summarized.
No-Amp Targeted Sequencing Library Preparation and Data Analysis Overview
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of the No-Amp Targeted Sequencing workflow and summarizes No-Amp library preparation & sequencing workflow details, No-Amp example performance data, No-Amp assay design procedures for analyzing custom targets, and No-Amp data analysis tools.
Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System, Sequel System
Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of the low DNA input workflow and summarizes experimental design considerations, low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Metagenomics Shotgun Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for metagenomics shotgun gene profiling & assembly applications and example performance data.
Full-Length 16S Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for full-length 16S applications and example performance data.
Multiplexed Microbial Library Preparation Using SMRTbell Express Template Prep Kit 2.0
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes multiplexed microbial sample preparation & sequencing workflow details, multiplexed microbial data analysis workflow details, and multiplexed microbial library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Single-Cell Iso-Seq Library Preparation Using SMRTbell Express TPK 2.0
Sequel II System, Sequel IIe System, Sequel System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes sample library preparation & sequencing workflow details for Single-Cell Iso-Seq applications and example performance data.
Multiplexed Library Preparation For Structural Variation Detection Using SMRTbell Express Template Prep Kit 2.0
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation describes multiplexed sample library preparation & sequencing workflow details for structural variation detection applications and multiplexed SV library example performance data (Sequel II System v8.0 / Chemistry 2.0). General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
Tutorial: Bioinformatics Lunch & Learn – HiFi Assembly
Sequel II System
Analysis
Description +
The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes. The long length and high accuracy of HiFi reads makes them the ideal starting point for many applications, and one area of major interest is genome assembly. HiFi assembly is faster, cheaper, more accurate, and easier to phase than standard long-read assembly. In this webcast, you will learn: The difference between long reads and HiFi reads; the strengths of HiFi read assembly for human, plant, animal, and insect genomes; step-by-step instruction on how to assemble HiFi reads using the publicly available FALCON package (https://github.com/PacificBiosciences/pb-assembly)
Sequel II System v8.0 & SMRT Link v8.0 Technical Overview
Sequel II System
Analysis, Binding, Sample Preparation, Sequencing, Template Preparation
Description +
This technical training presentation provides an overview of key features & benefits of the Sequel II System v8.0 and SMRT Link v8.0. 'Best Practices' overviews for sample library preparation and sequencing are also presented for whole genome sequencing for de novo assembly, whole genome sequencing for structural variation detection, variant detection using whole genome sequencing with HiFi reads, No-Amp targeted sequencing, long-read RNA sequencing, and metagenomic sequencing of complex populations.