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Training

Welcome to the PacBio training page. Here, you can search for and download online webinars, e-Modules, and other training documents. If you have any questions about our training materials, please contact us.

January 10, 2024

Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Kinnex library preparation for full-length 16S rRNA gene sequencing. Sequencing preparation workflow details for Kinnex 16S rRNA libraries as well as example sequencing performance data are also presented.
January 10, 2024

Technical overview — Kinnex library preparation using Kinnex full-length RNA kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Kinnex library preparation using the Kinnex full-length RNA kit. Sequencing preparation workflow details for Kinnex full-length RNA libraries as well as example sequencing performance data are also presented.
January 10, 2024

Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides a general overview of Kinnex library preparation using the Kinnex single-cell RNA kit, Kinnex full-length RNA kit and the Kinnex 16S rRNA kit. Sequencing preparation workflow details for Kinnex libraries as well as example sequencing performance data are also presented.
January 10, 2024

Technical overview — Revio ICS v13 and SMRT Link v13

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of key features & benefits of the new Revio system ICS v13 and SMRT Link v13 release.
June 13, 2023

Technical overview – MAS-Seq library preparation using the MAS-Seq for 10x Single Cell 3’ kit

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented. Step-by-step TSO artifact removal demo video is available.
April 5, 2023

Technical overview — Nanobind HT kits for automated HMW DNA extraction

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation

This technical training presentation describes Nanobind HT HMW DNA extraction kits for enabling high-throughput DNA sample preparation automation on four robotic platforms: Hamilton Microlab NIMBUS Presto, Thermo Fisher KingFisher Apex, Flex & Duo Prime systems. Example HiFi sequencing performance data for PacBio SMRTbell libraries constructed from Nanobind HT-extracted DNA are also presented.
November 7, 2022

Technical overview – Multiplexed amplicon library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of multiplexed amplicon library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0) in conjunction with barcoded primers, SMRTbell barcoded adapters or barcoded M13 primers. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations are also presented. Additional guidance for preparing multiplexed amplicon libraries for full-length 16S amplicon sequencing, Human Leukocyte Antigen (HLA) sequencing and HiFi target capture enrichment sequencing (using Twist Bioscience panels) is also summarized.
April 20, 2022

HiFi sequencing and software v11.0 release – Technical overview for Sequel II & Sequel IIe system users

  • Tutorial
  • Sequel II system, Sequel IIe system, Sequel II system and Sequel IIe system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of key features & benefits of the new HiFi sequencing and software v11.0 release for Sequel II and Sequel IIe systems. Topics covered include new consumables for SMRTbell library preparation & sequencing preparation (ABC) and SMRTbell library construction workflow updates for whole genome sequencing, viral sequencing, Iso-Seq analysis and multiplexed amplicon sequencing applications using the new SMRTbell prep kit 3.0. New instrument control software capabilities such as automated 5mC detection as well as other updated and new SMRT Link features in Sample Setup, Run Design, Run QC and SMRT Analysis are also discussed.
April 20, 2022

Technical overview – Multiplexed SARS-CoV-2 library preparation for full-viral genome sequencing using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II system, Sequel IIe system, Sequel II system and Sequel IIe system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of multiplexed library preparation for full-viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0) and the HiFiViral SARS-CoV-2 Kit for viral genome enrichment. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.
April 20, 2022

Technical overview – Iso-Seq library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II system, Sequel IIe system, Sequel II system and Sequel IIe system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of Iso-Seq library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing Iso-Seq samples using barcoded cDNA primers or SMRTbell barcoded adapters are discussed. Sequencing preparation workflow details, example performance data and Iso-Seq data analysis recommendations are also presented.
April 20, 2022

Technical overview – Whole genome and metagenome library preparation using SMRTbell prep kit 3.0

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation describes sample preparation details for constructing whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using SMRTbell prep kit 3.0 (SPK 3.0) for sequencing on PacBio Sequel II and Sequel IIe systems. Options for multiplexing WGS samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and data analysis recommendations for de novo assembly, variant detection and shotgun metagenomics are also presented.
April 20, 2022

Technical overview – Adeno-associated virus (AAV) library preparation using SMRTbell Prep Kit 3.0

  • Tutorial
  • Sequel II system, Sequel IIe system, Sequel II system and Sequel IIe system
  • Library preparation, Sample preparation, Binding, Sequencing, Analysis

This technical training presentation provides an overview of adeno-associated virus (AAV) library preparation for viral genome sequencing using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing AAV samples using SMRTbell barcoded adapters is discussed. Sequencing preparation workflow details, example sequencing performance data, and HiFiViral SARS-CoV-2 data analysis recommendations are also presented.
February 5, 2021

Ultra-Low DNA Input Library Preparation Using SMRTbell Express Template Prep Kit 2.0

  • Tutorial
  • Sequel II system and Sequel IIe system, Revio system
  • Library preparation, Sample preparation, Binding, Sequencing

This technical training presentation provides an overview of the ultra-low DNA input workflow and summarizes experimental design considerations, ultra-low DNA input sample QC requirements, low DNA input sample preparation & sequencing workflow details, and ultra-low DNA input library example performance data. General recommendations for high-molecular weight gDNA QC and handling for SMRTbell library construction are also presented.
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