Connect with a PacBio scientist

PacBio sequencing technology overview

PacBio offers three highly differentiated core technologies focused on accuracy, quality, and completeness:

HiFi sequencing

Single-molecule sequencing provides a combination of read lengths up to 25 Kb, remarkable read accuracy of 99.9%, and 5-base sequencing (A, T, G, C + 5mC)

Learn more

Sequencing by Binding (SBB)

SBB technology brings near-perfect accuracy — even in regions of the genome prone to sequencing errors

Learn more


Sample extraction technology protects DNA from shearing to generate longer fragments with less damage across a broader range of sample types than any other technology

Learn more

Sprite decoration
Roundel image of HiFi color logo

Long-read sequencing

HiFi sequencing

HiFi sequencing is recognized as a highly accurate and complete sequencing technology:

  • PacBio HiFi offers 21× fewer errors vs Oxford Nanopore, >6× fewer errors vs Illumina in PrecisionFDA benchmarking of total errors (SNV + indel + SV)
  • Read lengths up to 25 kb allow you to span large structural variants and challenging repetitive regions, and to sequence full-length transcripts
  • High accuracy provides 99.99% accurate sequencing results
  • Uniform coverage enables sequencing through regions that are inaccessible to other technologies
  • Epigenetics can be explored through direct detection of base modifications during sequencing

Sprite decoration



As the main finding, the authors in a new preprint entitled Utility of long-read sequencing for All of Us state that “HiFi reads produced the most accurate results for both small and large variants” and conclude that “long-reads have widespread value for establishing the most complete and accurate variant calls for All of Us and potentially for many other projects.”

Learn more

Sample prep

Nanobind HMW DNA extraction

NANOBIND sample extraction technology for high quality DNA that delivers longer fragments with less damage across a broader range of sample types than any other technology

  • Cultured cells and bacteria
  • Blood
  • Plants
  • Tissues
  • Developmental sample types include insects, fungal, and worm

Image of Nanobind consumables - PacBio

Sprite decoration
Roundel of SBB chemistry

Short-read sequencing

Sequencing by binding (SBB)

SBB demonstrates exceptional accuracy and consistency compared to current short-read sequencing-by-synthesis (SBS) technologies. An improved limit of detection, customer flexibility, and less computational burden have the potential to enable results in research.

Sprite decoration


A new paradigm: HiFi reads for highly accurate long-read sequencing

HiFi reads provide important benefits for key application areas including comprehensive variant detection, de novo assembly, epigenomics, metagenomics, and transcriptomics.

Read more

White Icon for instruments of Revio and Onso - PacBio


PacBio’s technology fuels our innovative products, including:

  • Revio sequencing system
  • Onso sequencing system
  • Nanobind extraction kits

Learn more

Data analysis

PacBio’s SMRT Link software suite is designed for use with HiFi sequencing data. Analyze, visualize, and manage your data through an intuitive GUI or command-line interface with an extensive set of APIs.

Learn more

white technologies icon

Sequencing methods

PacBio sequencing technology enables unparalleled resolution of genomes, transcriptomes, epigenomes, and targeted panels.

Learn more

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.