Technologies
Overview
PacBio offers these highly differentiated core technologies focused on accuracy, quality, and completeness
HiFi sequencing
Single-molecule sequencing provides a combination of read lengths up to 25 kb, remarkable read accuracy of 99.9%, and 5-base sequencing (A, T, G, C + 5mC)
Sequencing by binding
Innovative sequencing approach brings Q40+ base quality to a short-read sequencer — discover ultra-rare variants without compromises
Kinnex
Kinnex kits increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems
PureTarget
PCR-free libraries use the CRISPR/Cas9 system to target panels of medically-relevant genes and enable HiFi sequencing at scale
Nanobind DNA extraction
Sample extraction technology protects DNA from shearing to generate longer fragments with less damage across a broader range of sample types than other technologies
Long-read sequencing
HiFi sequencing
HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. PacBio HiFi reads is the only long read sequencing technology that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads, you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.
Short-read sequencing
Sequencing by binding (SBB)
Game-changing sequencing by binding (SBB) chemistry on the Onso system surpasses traditional sequencing by synthesis (SBS) technology. With near-perfect accuracy boasting 90% of bases at ≥Q40+, 15× higher accuracy, low duplication, negligible index hopping, and the ability to conquer challenging regions, SBB is redefining the landscape of short-read sequencing for research and translational applications.
PacBio has entered into an agreement to acquire Apton Biosystems, Inc. (Apton). As a combined organization, PacBio plans to integrate sequencing by binding (SBB) short-read chemistry into Apton’s high throughput short-read sequencer to enable the sequencing of billions of clusters of DNA on one flow cell. Stay tuned for updates.
Library prep
Kinnex
Kinnex is transforming the landscape of transcriptome research with isoform discovery and microbiome community profiling. Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase. Whether you are doing full-length RNA sequencing, single-cell RNA sequencing or 16S rRNA, Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.
Targeted sequencing
PureTarget
PureTarget uses the CRISPR-Cas9 system to generate targeted native DNA libraries. This amplification-free approach retains epigenetic signals and has no PCR artifacts or errors. With a streamlined workflow, multi-gene panels, and sample multiplexing, now you can screen the most challenging genomic regions at scale.
The PureTarget product line includes a panel of 20 repeat expansion loci relevant to neurodegenerative disease. Learn more about PureTarget-enabled comprehensive characterization of dark genes like FMR1, C9orf72 and RFC1.
Sample prep
Nanobind HMW DNA extraction
Nanobind extraction kits are based on proprietary Nanobind disks that can be used for the extraction of DNA from diverse sample types, including blood, cell, and tissue samples. With this technology, researchers can extract longer fragments from high-quality DNA in less than two hours, with minimal sample input and without the use of harmful chemicals or mechanical homogenization.
Products
Our technologies fuel our innovative products, including:
- Revio sequencing system
- Onso sequencing system
Sequencing methods
PacBio sequencing technology enables extraordinary resolution of genomes, transcriptomes, epigenomes, and targeted panels.
Data analysis
The SMRT Link software suite is designed for use with HiFi sequencing data. Analyze, visualize, and manage your data through an intuitive GUI or command-line interface with an extensive set of APIs.
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