Technologies
Overview
PacBio offers these highly differentiated core technologies focused on accuracy, quality, and completeness
HiFi sequencing
Single-molecule sequencing provides a combination of read lengths up to 25 kb, remarkable read accuracy of 99.9%, and 5-base sequencing (A, T, G, C + 5mC)
Kinnex
Kinnex kits increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems
Puretarget
PCR-free libraries use the CRISPR/Cas9 system to target panels of medically-relevant genes and enable HiFi sequencing at scale
Nanobind DNA extraction
Sample extraction technology protects DNA from shearing to generate longer fragments with less damage across a broader range of sample types than other technologies
Library prep
PCR-free library preparation for long-read HiFi sequencing that preserves native DNA and epigenetic signals
Long-read sequencing
HiFi sequencing
HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. PacBio HiFi reads is the only long read sequencing technology that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads, you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.
Targeted sequencing
PureTarget
PureTarget uses the CRISPR-Cas9 system to generate targeted native DNA libraries. This amplification-free approach retains epigenetic signals and has no PCR artifacts or errors. With a streamlined workflow, multi-gene panels, and sample multiplexing, now you can screen the most challenging genomic regions at scale.
The PureTarget product line includes a panel of 20 repeat expansion loci relevant to neurodegenerative disease. Learn more about PureTarget-enabled comprehensive characterization of dark genes like FMR1, C9orf72 and RFC1.
RNA sequencing
Kinnex
Kinnex is transforming the landscape of transcriptome research with isoform discovery and microbiome community profiling. Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase. Whether you are doing full-length RNA sequencing, single-cell RNA sequencing or 16S rRNA, Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.
Library prep
Library prep
PCR-free library preparation for long-read HiFi sequencing that preserves native DNA and epigenetic signals. Go from 500 ng of DNA to a sequencing-ready library in approximately 3.5 hours. Scale with automation-ready protocols and up to 384 available index adapters for high-throughput workflows
Sample prep
Nanobind HMW DNA extraction
Nanobind extraction kits are based on proprietary Nanobind disks that can be used for the extraction of DNA from diverse sample types, including blood, cell, and tissue samples. With this technology, researchers can extract longer fragments from high-quality DNA in less than two hours, with minimal sample input and without the use of harmful chemicals or mechanical homogenization.
Products
Our technologies fuel our innovative products, including:
- Revio sequencing system
- Onso sequencing system
Sequencing methods
PacBio sequencing technology enables extraordinary resolution of genomes, transcriptomes, epigenomes, and targeted panels.
Data analysis
The SMRT Link software suite is designed for use with HiFi sequencing data. Analyze, visualize, and manage your data through an intuitive GUI or command-line interface with an extensive set of APIs.
Accelerate your research