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Start your journey to discovery with SMRT consumables

PacBio provides complete workflows and all the consumable components you need to perform Single Molecule, Real-Time (SMRT) Sequencing.

 

 

Long, accurate reads are only a few steps away

 

 

Template Preparation and Multiplexing

  • Convert your samples (genomic DNA, cDNA or amplicons) into a primer-annealed SMRTbell library
  • Perform manually at the benchtop or by an automated liquid-handling system
  • Multiplex samples to improve efficiency

 

DNA Polymerase Binding and Cleanup

  • Bind primer-annealed SMRTbell templates to a DNA polymerase in preparation for sequencing

 

SMRT Sequencing

  • Utilize SMRT Cells and SMRT Sequencing reagents required for on-instrument sequencing
  • Order multi-well plates, septa, and tips specifically designed and optimized for use with PacBio Systems

 

Featured SMRT Consumables

Sequel II System Consumables including the SMRT Cell 8M

Introducing the new Sequel II Consumables to empower your research to the next level. Starting with the SMRT Cell 8M which contains approximately 8 million ZMWs to increase the sequencing throughput by ~8-fold. In addition to the SMRT Cell 8M, Sequel II Consumables consist of Sequel II Binding and Internal Control Kit 1.0 and Sequel II Sequencing Kit 1.0. Learn how these new consumables can elevate your whole genome sequencing, variant detection, and full-length RNA sequencing research with quick turnaround times to characterize SNPs, structural variants, splice isoforms, and improved genome annotation with high accuracy.

Learn more about the Sequel II System.

 


SMRTbell Express Template Prep Kit 2.0 and Iso-Seq Express Oligo Kit

Our new SMRTbell Express 2.0 product family now includes the Iso-Seq Express Oligo Kit (PN 101-737-500) to support a faster, streamlined full-length RNA sequencing workflow on the Sequel and Sequel II System at reduced cost.  With this new formulation, we have increased both the yield and efficiency of generating libraries using as little as 300 ng total RNA in one day.  The workflow also supports flexibility to modulate the size-distribution of full-length transcripts recovered in the tube without requiring bench-top size-selection.  We offer the option to multiplex samples for additional cost-savings to characterize isoform diversity in your transcriptome and improving reference genome annotations.

Learn More About Our Applications

SMRT Sequencing can sequence essentially any DNA strand: long, short or AT/GC rich across PacBio applications.

Whole Genome Sequencing

For humans, plants, animals and microbes including de novo sequencing and variant detection

Complex Populations

Understand variants among bacterial, viral and cancer cell populations

 

RNA Sequencing

In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes

Epigenetics

Detect DNA modifications in your samples while you sequence on the PacBio platform

Targeted Sequencing

Study relevant genome targets across any regions of interest

For more information about how our SMRT consumables can support your research, contact us.

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