Menu

HiFi Sequencing

HiFi reads for highly accurate long-read sequencing

Connect with a PacBio scientist

Tertiary hero overlay

Explore a new paradigm in sequencing with HiFi reads

Advanced scientific discoveries require sequencing data that is both accurate and complete. PacBio sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.

PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer compromise long read lengths for high accuracy sequencing to address your toughest biological questions.

How it works

Benefits of HiFi sequencing

Null

Long reads

Up to 25 kb in length

Null

High accuracy

Sequencing free of systematic errors

Null

Uniform coverage

No bias even for high GC regions

Null

Epigenetics

Direct methylation without special library prep

Video

Hear what our customers are saying about HiFi​

Discover how HiFi sequencing is transforming research directly from the scientists who use the technology. It’s more than just about long read lengths and high accuracy—it’s about making a real impact in the field.​

Breakthroughs in HiFi discovery

human y chromosome male - Photo of the eyes of two men looking at the camera.

HiFi enables researchers to complete and analyze the Y chromosome

Hifi difference isofrom print press feature image showing a commercial printing press in action

A HiFi movement: Transcript isoform resolved RNA-Seq

Directly phased epigenomes in every HiFi sequencing run

Sprite decoration

Blog

Sequencing 101: long-read sequencing

Long-read sequencing technologies such as PacBio HiFi sequencing are quickly becoming the new gold standard in genomics research. This article provides an introductory look at what long-read sequencing is, and explores topics including advantages, applications, and more.

Learn more

This is your moment for a more complete view of biology

Variants Icon representing variant calling

Comprehensive variant calling with phasing + 5mC

Access high accuracy for all variant types —  SNVs, indels, structural variants, tandem repeat expansions, and methylation — including in challenging regions.

RNA sequencing Icon representing RNA

Truly complete assembly of complex genomes

Accurately construct the full sequence of chromosomes, including telomeres and centromeres.

Targeted sequencing Icon representing targeted sequencing

Targeted sequencing to study genes at scale

Focus the power of HiFi variant calling by enriching for regions of interest using hybrid capture or PCR amplification.

Single-cell icon - Icon representing single-cell

Single-cell transcriptome sequencing

Catalog RNA isoforms at single-cell resolution, moving beyond gene counting to catalog full length transcripts.

How HiFi sequencing compares

HiFi sequencing SBS sequencing Nanopore sequencing
Read length 1–25 kb 2x150 bp 10–100 kb
Read accuracy 99.95% (Q33) 99.92% (Q31) 99.26% (Q21)
Run time

24 hours3

44 hours 72 hours
Yield

90 Gb2,5

2,400–3,000 Gb 50–110 Gb
Variant calling — SNVs
Variant calling — indels
X
Variant calling — SVs
X
5mC methylation
X
Phasing
X
  • 2HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0.
  • 3Run time specification is for the sequencing reaction.
  • 5HiFi yield is dependent on library fragment size. Yield is typically lower for shorter libraries.

HiFi Difference blog series

From eye-opening tech comparisons to inspiring examples of PacBio HiFi technology in action, this blog series explores what sets HiFi sequencing apart.

Sprite decoration

Explore HiFi sequencing systems

Learn how to choose the right system for your needs.

Learn more

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.