HiFi Sequencing

HiFi reads for highly accurate long-read sequencing

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HiFi sequencing

Unlock your next great discovery

With HiFi sequencing you get the benefits of short reads and traditional long reads in one easy-to-use technology. Watch this short video to learn how HiFi sequencing is empowering scientists to strive for new breakthroughs.

Explore a new paradigm in sequencing with HiFi reads

Advanced scientific discoveries require sequencing data that is both accurate and complete. PacBio sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.

PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.

How it works

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What are HiFi reads?

HiFi reads are produced using circular consensus sequencing (CCS) mode on PacBio long-read systems. HiFi reads provide base-level resolution with 99.9% single-molecule read accuracy.

HiFi reads can be used across a wide range of SMRT sequencing applications, from whole genome sequencing for de novo assembly, comprehensive variant detection, epigenetic characterization, RNA sequencing and more.

How are HiFi reads generated?

epigenetics hifi reads

 

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How does HiFi sequencing compare with other technologies?

HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. Explore the resources below for more information on how HiFi reads perform relative to other technologies.

Original publication: Wenger, A. M., et al. (2019) Accurate Circular Consensus Long-read Sequencing Improves Variant Detection and Assembly of a Human Genome. Nature Biotechnology, 37, 1155–1162.

Telomere-to-Telomere

The Telomere-to-Telomere (T2T) Consortium selected HiFi sequencing to generate the first fully complete human genome assembly, 20 years after the original human genome project.

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HiFi reads

HiFi reads make understanding the accuracy of your sequencing data easy and straightforward, but there is a lot to know about accuracy when considering a sequencing technology.

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precisionFDA

In the precisionFDA Truth Challenge V2, which evaluated methods for variant calling in human genomes, approaches that use HiFi reads delivered the highest precision and recall in all categories.

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What are the benefits of HiFi sequencing?

There are many advantage to using HiFi sequencing beyond the long read lengths and high accuracy. These include easy library preparation, low coverage requirements, small file sizes, and the fastest time to assembly. Explore the resources below better understand these advantages.

Brochure

Learn more about the benefits of HiFi reads and see how they provide a single technology solution across a range of applications

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