Explore a new paradigm in sequencing with HiFi reads
Advanced scientific discoveries require sequencing data that is both accurate and complete. PacBio sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.
PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer compromise long read lengths for high accuracy sequencing to address your toughest biological questions.
Benefits of HiFi sequencing
Long reads
Up to 25 kb in length
High accuracy
Sequencing free of systematic errors
Uniform coverage
No bias even for high GC regions
Epigenetics
Direct methylation without special library prep
Video
Hear what our customers are saying about HiFi
Discover how HiFi sequencing is transforming research directly from the scientists who use the technology. It’s more than just about long read lengths and high accuracy—it’s about making a real impact in the field.
Breakthroughs in HiFi discovery
Blog
Sequencing 101: long-read sequencing
Long-read sequencing technologies such as PacBio HiFi sequencing are quickly becoming the new gold standard in genomics research. This article provides an introductory look at what long-read sequencing is, and explores topics including advantages, applications, and more.
This is your moment for a more complete view of biology
Comprehensive variant calling with phasing + 5mC
Access high accuracy for all variant types — SNVs, indels, structural variants, tandem repeat expansions, and methylation — including in challenging regions.
Truly complete assembly of complex genomes
Accurately construct the full sequence of chromosomes, including telomeres and centromeres.
Targeted sequencing to study genes at scale
Focus the power of HiFi variant calling by enriching for regions of interest using hybrid capture or PCR amplification.
Single-cell transcriptome sequencing
Catalog RNA isoforms at single-cell resolution, moving beyond gene counting to catalog full length transcripts.
How HiFi sequencing compares
HiFi Difference blog series
From eye-opening tech comparisons to inspiring examples of PacBio HiFi technology in action, this blog series explores what sets HiFi sequencing apart.
