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HIFI SEQUENCING

DELIVERING HIGHLY ACCURATE LONG READS TO DRIVE DISCOVERY IN LIFE SCIENCE

What is SMRT sequencing?

Single Molecule, Real-Time (SMRT) sequencing is the core technology powering our long-read sequencing platforms. This innovative approach was the first of its kind and is now a proven technology used in all fields of life science.

Original publication: Eid, J., et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.

What are the advantages of SMRT sequencing?

Long reads

With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. SMRT sequencing provides exceptional read lengths without compromising throughput or accuracy.

Data from a 15 kb size-selected human library using the SMRTbell express template prep kit 2.0 on a Sequel IIe system (2.0 chemistry, Sequel IIe system software v10, 30-hour movie). Read lengths, reads/data per SMRT Cell, and other sequencing performance results vary based on sample quality/type and insert size.

hifi sequencing read length graph - PacBio

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Product_Sequel IIe System_High Accuracy - PacBio

High accuracy

Sequencing free of systematic error achieves >99.999% consensus accuracy. Explore the benefits of highly accurate long-read sequencing. With unmatched accuracy, low sequencing-context bias, and accurate mapping of reads, SMRT sequencing provides the information needed to confidently call and detect all variants.

Data from a 15 kb size-selected human library using the SMRTbell express template prep kit 2.0 on a Sequel IIe system (2.0 chemistry, Sequel IIe system software v10, 30-hour movie). Read lengths, reads/data per SMRT Cell, and other sequencing performance results vary based on sample quality/type and insert size.

Uniform coverage

No bias based on GC content means you can sequence through regions inaccessible to other technologies. Readily sequence through AT-rich or GC-rich regions, highly repetitive sequences, long homopolymers, and palindromic sequences with SMRT sequencing.

Mean coverage per GC window across a human sample. Data generated with a 20 kb HiFi library on a Sequel II system (2.0 chemistry and Sequel II system). Read lengths, reads/data per SMRT Cell, and other sequencing performance results vary based on sample quality/type and insert size.

Product_Sequel IIe System_Uniform Coverage - PacBio

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Product_Sequel IIe System_Accuracy vs Read Length - PacBio

Single-molecule resolution

Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99.9% single-molecule accuracy. Readily achieve high-quality reads to confidently resolve variants of all types.

Data from a 15 kb size-selected human library using the SMRTbell express template prep kit 2.0 on a Sequel IIe system (2.0 chemistry, Sequel IIe system software v10, 30-hour movie). Read lengths, reads/data per SMRT Cell, and other sequencing performance results vary based on sample quality/type and insert size.

Epigenetics

With no PCR amplification step, base modifications are directly detected during sequencing. Measurement of variation in polymerase kinetics of DNA base incorporation eliminates the need for chemical modification to detect base modifications. This allows you to capture sequence and epigenetic information in a single experiment.

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