PacBio Literature

PacBio in collaboration with Covaris has developed a workflow that enables extraction, preparation, and sequencing of FFPE-derived DNA for HiFi sequencing. This approach overcomes traditional limitations and unlocks FFPE samples for high-resolution, long-read genomic analysis.

Highly accurate long-read RNA sequencing reveals novel mechanisms of RNA dysregulation in cancer

Affordably scale your HiFi sequencing with SPRQ-Nx chemistry to generate more complete genomes, full-length transcriptomes, and metagenomes, while detecting more variant types and direct methylation. Achieve greater resolution in your projects with the exceptional HiFi accuracy of 90% of bases at Q30+. Enjoy on-instrument primary analysis with automatic conversion to the standard BAM format – so you can make meaningful insights faster.

The Vega system is an innovative Revio technology, reimagined in a benchtop solution with seamless integration with HiFi library prep workflows and SMRT Link run management software. On-instrument primary analysis, including DeepConsensus, methylation calling, and optional barcode demultiplexing in every run.

Understanding the complexity of cancer genomes requires methods that can reveal the full breadth of cancer genomic variation, from SNVs and indels to structural variants (SVs), copy number variants (CNVs), and differential methylation. HiFi long-read sequencing allows cancer researchers to characterize the complete spectrum of somatic and germline variation, including SVs and methylation, yielding a clearer view of cancer biology and helping to identify new therapeutic targets and inform clinical decision making.

Comprehensive genome sequencing data integrated with chromatin interaction information is crucial for complete, chromosome-scale genome assembly. CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.

Highly accurate long reads — HiFi reads — are the new benchmark for full-length 16S, metagenome profiling, and metagenome assembly. Determine community composition at the species or strain level with competitively priced full-length 16S sequencing. Generate hundreds of high-quality (HQ) metagenome assembled genomes (MAGs), many of which are circular single contigs. Profile metagenomes efficiently and cost-effectively with an average of eight full-length genes in every HiFi read. Leverage epigenomic data to associate contigs and plasmids from closely related strains.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost including microbial whole genome sequencing, full-length 16s/ITS sequencing, viral sequencing and shotgun metagenome sequencing.

Make reference-quality genomes your new standard practice with highly accurate long reads—HiFi reads—and the PacBio end-to-end workflow. Generate closed chromosomes and plasmids. Identify ever-evolving genes associated with toxicity, virulence, and antimicrobial resistance. Precisely identify strains, serotypes, and plasmids to help track pathogen outbreaks in human, plant, and animal species, through food systems, hospitals, and communities. More comprehensively characterize microbes to facilitate scientific breakthroughs and discovery

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

PacBio-developed tools offer an excellent analysis experience as they are designed specifically for HiFi data. There are multiple ways to access PacBio analyses.

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.