PacBio Literature

PacBio long-read HiFi sequencing allows you to sequence the entire AAV genome with extraordinary accuracy and completeness so that you can quickly discover and optimize gene therapy vectors.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

In this whitepaper, we discuss the features of bulk and single-cell isoform sequencing with the Iso-Seq method and their applications in human genomics.

The LightBench from Yourgene Health uses Ranger Technology to deliver accurate size selection for whole genome sequencing and includes the ability to perform fragment length analysis for additional quality control. With an automated workflow to optimize walk-away time, the LightBench offers a scalable size selection solution for the preparation of SMRTbell gDNA libraries for HiFi sequencing.

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

This application note details a wide array of bioinformatics tools for full length- isoform sequencing across isoform classification and annotation, fusion transcript discovery, variant calling, transcript assembly, differential transcript analysis, differential splicing usage, and transcript visualization.

The Kinnex full-length RNA kit takes total RNA as input and outputs a sequencing-ready library that results in an 8-fold throughput increase compared to typical Iso- Seq libraries. Combined with the Iso-Seq analysis in SMRT Link software, PacBio offers cost-effective isoform sequencing that does not require orthogonal sequencing methods.

The Kinnex single-cell RNA kit takes as input single- cell cDNA and outputs a sequencing-ready library that results in a 16-fold throughput increase compared to regular single-cell Iso-Seq libraries.

PacBio® HiFi sequencing delivers highly accurate long reads for analyzing both DNA and RNA. The unique combination of accurate base calling and long read length of HiFi enable the phasing of haplotypes, coverage of high GC regions, and bisulfite-free detection of 5-methylcytosine. Together, these features allow you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before

The Revio system is built for core labs who want to scale. HiFi sequencing on the PacBio Revio system brings higher throughput and reduced cost to labs where scale is important.

Long read lengths and high accuracy are essential components to successful whole transcriptome analysis. PacBio full-length RNA sequencing demonstrates a comparative advantage against ONT.

Powered by sequencing by binding (SBB) chemistry, the PacBio short- read Onso platform delivers extraordinary base-calling accuracy (90% of bases at ≥Q40), setting a new standard in genetic research. This remarkable accuracy instills a new level of confidence in sequencing data and improves the ability to characterize challenging genetic variations through genome- wide and targeted approaches.

The PacBio® Compatible program provides seamless integration of industry-leading tools into the short-read sequencing by binding (SBBTM) workflow on the OnsoTM platform.

This Application note introduces the PacBio WGS Variant Pipeline that consolidates several state-of-the- art analysis tools into a single user-friendly workflow.

This technical note describes one set of experimental conditions for shearing high molecular weight genomic DNA (gDNA) derived from human whole blood using MP Biomedicals’ FastPrep-96 instrument. These results demonstrate the FastPrep-96 as a high-throughput DNA shearing option for WGS.