PacBio Literature

In this Application note, we focus on an amplicon- sequencing based approach for the analysis of the entire GBA gene and its pseudogene with PacBio® HiFi sequencing. We also highlight the benefits of RNA isoform sequencing (Iso-Seq® method) to generate insights into the transcriptional impact of GBAP1.

PacBio HiFi reads sequence full-length RNA isoforms along with single-cell barcode and UMI information, revealing isoform diversity at the single-cell level.

Getting the most out of your long-read whole genome sequencing (WGS) workflow requires accurately characterizing the size distribution of your genomic DNA and SMRTbell® libraries. The Agilent Femto Pulse system is the recommended quality control (QC) technology for PacBio long-read sequencing using the new SMRTbell prep kit 3.0 workflow

In this Application Note, we focus on HiFi sequencing of full-length PCR amplicons spanning the 3.3 kb CYP21A2 and its pseudogene, CYP21A1P. PacBio HiFi reads are long (up to 25 kb) and accurate (99.9%) making them well suited to analyze the complexity of CAH-related pathogenic variants, where gene conversions and chimeric genes are the predominant mutation types.

With PacBio® HiFi sequencing, CYP2D6 variation can be captured using highly accurate long reads on a single platform. Amplicon-based HiFi sequencing fully and directly resolves and phases complex loci like CYP2D6 without assembly or inference, allowing for ancestry agnostic haplotype assignment.

PacBio® HiFi reads are long (up to 25 kb) and accurate (99.9%). Targeted HiFi sequencing of amplicons has the ability to span entire genes, allowing for straightforward haplotype construction, detection of structural variants or copy number variants in addition to SNVs and indels. These attributes make targeted HiFi sequencing well suited to analyze both rare and common variants of all types, thus increasing the positive detection rate of thalassemia-related variants.

PacBio® HiFi sequencing delivers highly accurate long reads for analyzing both DNA and RNA. The unique combination of accurate base calling and long read length of HiFi enable the phasing of haplotypes, coverage of high GC regions, and bisulfite-free detection of 5-methylcytosine. Together, these features allow you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before

SBB® is an innovative sequencing technology that delivers amazing NGS accuracy. By incorporating native nucleotides and removing the need to cleave fluorescent modifications, the sequencing strand has minimal scarring and results in higher accuracy base calls.

Powered by the innovative sequencing by binding (SBB®) technology, the Onso system delivers unprecedented accuracy and sensitivity to advance your critical and groundbreaking research.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an unprecedented level of accuracy through unique sequencing by binding (SBB®) technology. The Onso system features dual lanes, no index-hopping, and low duplications.

See it all with HiFi sequencing on the Revio™ system

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

HiFi reads can span most repeats, making it possible to comprehensively profile variation in repeat regions and to measure any changes in methylation. PacBio has developed the Tandem repeat genotyping tool (TRGT) and Tandem repeat visualizer (TRVZ) as bioinformatics tools to accurately profile short tandem repeats, variable number tandem repeats, and macrosatellites.

Highly accurate long-read RNA sequencing reveals novel mechanisms of RNA dysregulation in cancer

Gene editing and HiFi sequencing provide fast, reliable solutions that can accelerate the breeding process, modify target genes to improve traits, improve plant resistances to biotic and abiotic stresses and increase plant architecture to better resist climate change.