PacBio Literature

Powered by the innovative sequencing by binding (SBB®) technology, the Onso system delivers unprecedented accuracy and sensitivity to advance your critical and groundbreaking research.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an unprecedented level of accuracy through unique sequencing by binding (SBB®) technology. The Onso system features dual lanes, no index-hopping, and low duplications.

See it all with HiFi sequencing on the Revio™ system

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

HiFi reads can span most repeats, making it possible to comprehensively profile variation in repeat regions and to measure any changes in methylation. PacBio has developed the Tandem repeat genotyping tool (TRGT) and Tandem repeat visualizer (TRVZ) as bioinformatics tools to accurately profile short tandem repeats, variable number tandem repeats, and macrosatellites.

Highly accurate long-read RNA sequencing reveals novel mechanisms of RNA dysregulation in cancer

PacBio HiFi reads sequence full-length RNA isoforms along with single-cell barcode and UMI information, revealing isoform diversity at the single-cell level.

In this Application Note, we focus on HiFi sequencing of full-length PCR amplicons spanning the 3.3 kb CYP21A2 and its pseudogene, CYP21A1P. PacBio HiFi reads are long (up to 25 kb) and accurate (99.9%) making them well suited to analyze the complexity of CAH-related pathogenic variants, where gene conversions and chimeric genes are the predominant mutation types.

PacBio® HiFi reads are long (up to 25 kb) and accurate (99.9%). Targeted HiFi sequencing of amplicons has the ability to span entire genes, allowing for straightforward haplotype construction, detection of structural variants or copy number variants in addition to SNVs and indels. These attributes make targeted HiFi sequencing well suited to analyze both rare and common variants of all types, thus increasing the positive detection rate of thalassemia-related variants.

PacBio® HiFi sequencing delivers highly accurate long reads for analyzing both DNA and RNA. The unique combination of accurate base calling and long read length of HiFi enable the phasing of haplotypes, coverage of high GC regions, and bisulfite-free detection of 5-methylcytosine. Together, these features allow you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before

Gene editing and HiFi sequencing provide fast, reliable solutions that can accelerate the breeding process, modify target genes to improve traits, improve plant resistances to biotic and abiotic stresses and increase plant architecture to better resist climate change.

With PacBio® HiFi sequencing, CYP2D6 variation can be captured using highly accurate long reads on a single platform. Amplicon-based HiFi sequencing fully and directly resolves and phases complex loci like CYP2D6 without assembly or inference, allowing for ancestry agnostic haplotype assignment.

Extract HMW DNA for HiFi sequencing for one-stop sample-to-answer workflow and support

The HiFiViral SARS-CoV-2 kit is an end-to-end solution for genomic surveillance of COVID-19 variants. The kit utilizes molecular inversion probes (MIPs), a differentiated enrichment technology which combines the simplicity and cost-effectiveness of PCR with the resilience to mutations of target capture. The MIPs approach, coupled with PacBio HiFi reads and SMRT® Link software analysis, delivers several advantages over PCR amplicon sequencing

AMPure® PB bead size selection is a simple, quick, and scalable approach to deplete molecules less than 5 kb in a SMRTbell® library. For high-quality DNA, the sequencing performance and application metrics for assembly and variant calling are comparable to gel cassette size-selection methods.