PacBio Literature

Other sequencing methods suffer from shortcomings like an inability to capture the backbone sequence or low accuracy in difficult genomic regions such as homopolymers or regions of high GC content. PacBio® HiFi sequencing overcomes these limitations and allows you to capture the full plasmid sequence, including backbone and difficult regions.

The whole genome sequencing (WGS) application requires an up-front mechanical DNA shearing step to transform genomic DNA into fragment sizes appropriate for HiFi sequencing. With the Hamilton Microlab Prep system, this step can be performed in as little as 22 minutes for 24 samples at the cost of a pipette tip per sample. Here we describe the required equipment and consumables, best practices, and procedure for shearing DNA on the Microlab Prep system.

Fiber-seq is a powerful new long-read whole genome sequencing assay that overcomes the challenges imposed by short-read chromatin sequencing.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.

HiFi systems brochure

Application brief — What can you do with one SMRT Cell

Vega benchtop system brochure

See it all with HiFi sequencing on the Revio™ system

This application note details the PureTarget carrier panel targets, presents example performance, and provides a technical overview of the methods, workflow, and analysis pipeline.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

The Iso-Seq method, full-length RNA sequencing using PacBio HiFi sequencing, can sequence full- length isoforms without the need for computational inferencing, reducing errors in isoform identification. The KinnexTM RNA kits, based on the MAS-Seq method for concatenating amplicons such as cDNA, further increases throughput on the PacBio long-read sequencers. Here, we highlight publications using Iso- Seq in neuroscience research to show how bulk and single-cell Iso-Seq data help identify novel isoforms, determine disease-specific isoform expressions, delineate splicing patterns during brain development, and help identify new drug candidates.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

The Onso short-read sequencing system is a scalable and flexible benchtop platform that provides an extraordinary level of accuracy through its unique sequencing by binding (SBBTM) technology.