PacBio Literature

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

HiFi systems brochure

See it all with HiFi sequencing on the Revio™ system

With an average of eight complete genes per HiFi read, PacBio® data provides rich functional information; nearly every read contributes to the understanding of the biological functions present in the microbial community

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

PacBio in collaboration with Covaris has developed a workflow that enables extraction, preparation, and sequencing of FFPE-derived DNA for HiFi sequencing. This approach overcomes traditional limitations and unlocks FFPE samples for high-resolution, long-read genomic analysis.

Highly accurate long-read RNA sequencing reveals novel mechanisms of RNA dysregulation in cancer

Affordably scale your HiFi sequencing with SPRQ-Nx chemistry to generate more complete genomes, full-length transcriptomes, and metagenomes, while detecting more variant types and direct methylation. Achieve greater resolution in your projects with the exceptional HiFi accuracy of 90% of bases at Q30+. Enjoy on-instrument primary analysis with automatic conversion to the standard BAM format – so you can make meaningful insights faster.

The Vega system is an innovative Revio technology, reimagined in a benchtop solution with seamless integration with HiFi library prep workflows and SMRT Link run management software. On-instrument primary analysis, including DeepConsensus, methylation calling, and optional barcode demultiplexing in every run.

Understanding the complexity of cancer genomes requires methods that can reveal the full breadth of cancer genomic variation, from SNVs and indels to structural variants (SVs), copy number variants (CNVs), and differential methylation. HiFi long-read sequencing allows cancer researchers to characterize the complete spectrum of somatic and germline variation, including SVs and methylation, yielding a clearer view of cancer biology and helping to identify new therapeutic targets and inform clinical decision making.

Comprehensive genome sequencing data integrated with chromatin interaction information is crucial for complete, chromosome-scale genome assembly. CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.