PacBio Literature

This Application note introduces the PacBio WGS Variant Pipeline that consolidates several state-of-the- art analysis tools into a single user-friendly workflow.

The Vega system is an innovative Revio technology, reimagined in a benchtop solution with seamless integration with HiFi library prep workflows and SMRT Link run management software. On-instrument primary analysis, including DeepConsensus, methylation calling, and optional barcode demultiplexing in every run. SPRQ-Nx chemistry enables lower cost per run, 90 Gb yield, single-shift movies, and supports 21 CFR Part 11 compliance.

Login mode can be enabled per instrument to require users to log in with their SMRT Link username and a personal identification code (PIC). SMRT Link captures audit trails across both software and instrument activity. • Ensures actions are tied to a specific user • Extends user accountability to on-instrument activity • User management limited to admins. SMRT Link captures audit trails across both software and instrument activity.

Affordably scale your HiFi sequencing with SPRQ-Nx chemistry to generate more complete genomes, full-length transcriptomes, and metagenomes, while detecting more variant types and direct methylation. Achieve greater resolution in your projects with the exceptional HiFi accuracy of 90% of bases at Q30+. Enjoy on-instrument primary analysis with automatic conversion to the standard BAM format – so you can make meaningful insights faster.

With PacBio long reads you can easily and cost-effectively sequence the full length of amplicons that target genes or regions of interest, from several hundred base pairs to kilobase scale. Highly accurate HiFi reads allow you to target across types of variation, from single nucleotide variants and indels, to structural variants. It also enables unambiguous haplotype resolution through direct phasing. HiFi sequencing can reveal biology that other technologies miss, like low-complexity regions, repeat expansions, GC-rich promoter regions, and insertion sites of transposable elements.

A streamlined targeted sequencing approach for existing PCR assays is to use indexed SMRTbell adapters. With the automated HiFi plex prep kit, prepare individual SMRTbell libraries for each sample, pool, and sequence on one SMRT Cell.

With highly accurate long reads (HiFi reads), powered by Single Molecule, Real-Time (SMRT) sequencing technology, you can more comprehensively validate gene editing techniques such as CRISPR-Cas9 approaches.

Sequencing has become an integral part of the biopharma research and development process and enhances our understanding of the molecular determinants of health and disease. Whether you are conducting research on novel potential drug targets, biomarkers, or therapeutics such as gene therapies, PacBio® offers highly accurate sequencing solutions across its portfolio that can improve your chances of success and may reduce your time to market.

HiFi systems brochure

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

See it all with HiFi sequencing on the Revio™ system

With an average of eight complete genes per HiFi read, PacBio® data provides rich functional information; nearly every read contributes to the understanding of the biological functions present in the microbial community