September 16, 2026 | Microbial sequencing methods
Asset Type: Technical note
May 20, 2025 | Sample + library preparation
Technical note – Concatenating cDNA or gDNA amplicons using Kinnex kits to increase throughput
February 18, 2025 | Sample + library preparation
Technical note – Preparing Kinnex single-cell libraries with Twist exome enrichment kits
February 10, 2025 | Sample + library preparation
Technical note – Targeted enrichment of Kinnex full-length RNA libraries with IDT xGen hybridization and wash kit
December 11, 2024 | Sample + library preparation
Technical note — HiFi sequencing performance of saliva DNA samples collected with DNA Genotek Oragene devices and extracted using Nanobind kits
May 23, 2024 | Sample + library preparation
Technical note — Preparing Kinnex single-cell libraries with Parse Evercode Whole Transcriptome kits
February 23, 2024 | Sample + library preparation
Technical note — Extracting HMW DNA from diverse insect samples using the Nanobind PanDNA kit for optimal HiFi sequencing
January 16, 2024 | Sample + library preparation
Technical note — High throughput DNA shearing using Hamilton Microlab Prep
November 16, 2023 | Sample + library preparation
Technical note — Size selection of PacBio SMRTbell libraries with the Lightbench instrument from Yourgene Health
September 21, 2023 | Sample + library preparation
Technical note — High throughput DNA shearing for HiFi whole genome sequencing from whole blood samples
June 30, 2022 | Sample + library preparation
Technical note — HiFi WGS Performance with AMPure PB bead size selection
AMPure® PB bead size selection is a simple, quick, and scalable approach to deplete molecules less than 5 kb in a SMRTbell® library. For high-quality DNA, the sequencing performance and application metrics for assembly and variant calling are comparable to gel cassette size-selection methods.
April 20, 2022 | Sample + library preparation
Technical note — Gel cassette size selection methods for WGS HiFi libraries
PacBio recommends to use size-selection beads are to remove short DNA fragments and enrich for the long fragments that are most valuable for downstream analysis. Although size-selection beads have many advantages, alternative methods — like gels and DNA precipitation — offer more flexibility in defining a size cutoff. This technical note provides guidance for alternative methods of size selection of WGS libraries prepared with the SMRTbell prep kit 3.0.
April 20, 2022 | Human genetics research
Technical note — Human whole blood sample prep
Sample preparation is a critical factor for whole genome sequencing (WGS) projects that impacts sequencing yield, read length, and ultimately performance for variant calling and genome assembly. Best practices to optimize performance for PacBio HiFi sequencing are to collect whole human blood, store with potassium EDTA as the anticoagulant for fewer than 2 days at 4°C, and extract DNA from 200 μL of blood using the Nanobind CBB Big DNA kit. When it is not possible to extract from fresh samples, storing samples at 4°C maximizes extraction yield and minimizes degradation for at least 9 days.