Plant + animal genomics solutions
In the face of a changing climate and growing populations, highly accurate long-read sequencing technology gives plant and animal scientists access to actionable genomic insights that other technologies cannot provide.
Accelerate breeding programs and increase resilience to pests, disease, and climate challenges.
Explore the biodiversity of populations, species and ecosystems to build a more detailed view of the biome.
Plant + animal genomics in action
Genome wide detection and phasing of genetic and epigenetic variants from a single library prep
Simultaneously measure methylation by detecting 5mC at CpG sites.
Building haplotype-resolved pangenome databases for improved marker development
With third-generation sequencing tools, researchers have been able to better impute complex traits…
Getting the most out of your breeding program with sequencing
Three plant biologists talk about their journeys into sequencing plant genomes…
Chromosome-Level Assembly of Flowering Cherry ( Prunus campanulata) Provides Insight into Anthocyanin Accumulation
The flowering cherries (genus Prunus, subgenus Cerasus) are popular ornamental trees that produce bell-shaped flowers with colors ranging from bright pink to crimson during the Spring Festival from January to March each year in many Asian countries. The P. campanulata cultivar “Lianmeiren”, with only 0.54% of heterozygosity, was the focus of this study, and generated a high-quality chromosome-scale genome assembly of P. campanulata by combining Pacific Biosciences (PacBio) single-molecule sequencing, 10× Genomics sequencing, and high-throughput chromosome conformation capture (Hi-C) technology.
Shaping the future
Sequoia sempervirens is one of the world’s fastest-growing conifers that live for hundreds of years. Once ubiquitous throughout the Northern Hemisphere, now only 5% of the original old-growth coastal redwood forest remains. Explore how highly accurate long-read sequencing successfully sequenced the large and highly complex California redwood genome.
Advantages of using HiFi sequencing for your plant + animal genomics research
Highly accurate genomic sequencing helps uncover genomic clues from the organisms that populate our world. Complex genomes and transcriptomes provide pathways to protect crop and livestock health and better understand plant and animal biology at a molecular level.
Here are some advantages of HiFi sequencing to support your plant and animal research:
- Build more complete, accurate, and phased reference genomes for all types of organisms
- Confidently call variants at every scale, including SNPs, structural variants, copy number variants, translocations, and homopolymer repeats
- Uncover new biology with RNA sequencing that provides full-length, phased transcript sequence — profile isoforms and capture 5’ untranslated regions
- Reveal previously hidden regulatory mechanisms with epigenetic sequence information
Applications to fuel plant + animal genomics research
Assemble haplotype-resolved, complete, and accurate de novo genomes and simultaneously detect methylation in the face of challenging complexity, such as high ploidy, high GC content, and highly repetitive sequences.
Resolve variants at every scale — from SNPs and structural variants to copy number variants and translocations.
Accurately detect and uncover all variant types even in hard-to-reach regions of the genome to power genotyping studies, breeding programs, and more.
PacBio’s SMRT Grant program supports outstanding research that advances scientific discovery by awarding free long-read sequencing resources to grant recipients
Our 2021 Plant + Animal SMRT Grant program asked applicants to “Zoom in on genetic variation for a better future.” There were hundreds of applicants interested in using HiFi sequencing to obtain haplotype-resolved pangenomes to impute traits, capture structural variants, and decipher complex traits. Find out who won this year’s grant by pursuing a safer, tastier future.
Long-read sequencing helps answer biological questions across a broad range of plant + animal sciences
Breaking through barriers of polyploidy and heterozygosity
Develop disease prevention methods and improve pest control.
Improve crop health, conduct trait selection, and enhance breeding efficiency.
Animal health + breeding
Study patterns of inheritance, immunology and domestication.
Workflows for your plant + animal research
Easy-to-use, high-throughput sequencing for the accuracy you need at an affordable cost
Flexible DNA input requirements down to 300 ng
Adjustable multiplexing options to maximize throughput
Run a single SMRT Cell 8M at a time or several consecutively for set it and forget it sequencing
Use SMRT Link software, or open source tools to analyze your HiFi data