Application kits

Reagents and oligos for generating MAS-Seq libraries from 10x Chromium Single Cell 3' cDNA (8 reactions). Includes MAS primers & adapters, capture beads, and SMRTbell cleanup beads that produce a library ready for sequencing. Sold as a bundle only. See resources below for TSO artifact removal demo video.

8 reactions

Sequel II and IIe Systems compatible.

• Resource: Procedure & Checklist - Preparing MAS-Seq libraries using MAS-Seq for 10x Single Cell 3' kit
• Resource: TSO artifact removal demo - VIDEO
• Resource: MAS-Seq for Single Cell Bioinformatics Walkthrough
• Product insert: MAS-Seq for 10x 3' concatenation kit
• Product insert: MAS capture beads kit
• Product insert: SMRTbell cleanup beads
• SDS: MAS-Seq for 10x 3' concatenation kit
• SDS: MAS capture beads kit
• SDS: SMRTbell cleanup Beads

The HiFiViral SARS-CoV-2 kit contains reagents for enrichment and cDNA synthesis of SARS-CoV-2 RNA (384 samples). Also included are 384 barcoded primer pairs in plate format (1 sample per barcoded primer pair).

Note: Sequel II and IIe Systems compatible.

• Resource: Procedure & Checklist – PacBio HiFiViral High-Throughput Multiplexing for Full-Viral Genome Sequencing of SARS-CoV-2
• Resource: Plate layout and primer sequences
• Resource: Primer sequences (FASTA)
• Product insert: HiFiViral SARS-CoV-2 kit
• Product insert: Barcoded M13 primer plate (384 primer pairs, 384 samples)
• SDS: HiFiViral SARS-CoV-2 kit
• SDS: Barcoded M13 primer plate (384 primer pairs, 384 samples)

The SMRTbell gDNA amplification kit contains reagents to amplify gDNA to a level sufficient for SMRTbell library preparation (18 samples).

Note: Compatible with the Sequel II and Sequel IIe Systems

• Resource: Procedure & Checklist - Preparing HiFi SMRTbell Libraries from Ultra-Low DNA Input
• Resource: Application Note Considerations for Using the Low and Ultra-low DNA Input Workflows for Whole Genome Sequencing
• Product insert: SMRTbell gDNA amplification kit
• SDS: SMRTbell gDNA amplification kit

The Iso-Seq express oligo kit contains template switching oligo (TSO) and cDNA PCR primer for Iso-Seq library preparation (24 samples).

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

• Resource: Procedure & Checklist: Iso-Seq Express Template Preparation for Sequel and Sequel II and Sequel IIe Systems
• Product insert: Iso-Seq express oligo kit
• SDS: Iso-Seq express oligo kit