MAS-Seq for Single Cell Bioinformatics Walkthrough
In this tutorial, we demonstrate how to use PacBio’s SMRT Link software to analyze MAS-Seq single cell data to produce gene- and isoform-level count matrices that can be used with tertiary analysis tools such as Kana, Seurat, and CellTypist for cell clustering and cell labeling.
TSO artifact removal demo | MAS-Seq for 10x Single Cell 3’ kit
The TSO (template switching oligo) cDNA artifact removal step is demonstrated here in this video. Learn more about the PacBio MAS-Seq method for single-cell isoform sequencing.