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Kinnex

Scalable, cost-effective RNA sequencing with increased throughput

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What is Kinnex?

The Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase.

Kinnex kits increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems to enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.

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Long-read value for RNA applications

Application Full-length RNA Single-cell RNA 16S rRNA
Sequencing goal Isoform discovery and abundance information Cell type-specific isoform isoform discovery and abundance information Species identification in metagenomics samples
Short-read shortcomings Incorrect isoform discovery and abundance information No isoforms. Only genes No species identification
Other long-read drawbacks

Lower accuracy poor transcript identification and variant calling

Lower accuracy poor transcript identification and variant calling

Lower accuracy poor transcript identification and variant calling

HiFi advantages Isoform discovery and abundance information Cell type-specific isoform discovery Species-level identification
Read the competitive whitepaper Read the competitive whitepaper Read the app note

“The new family of Kinnex kits for RNA sequencing are designed to provide customers with cost-effective, end-to-end kitted solutions to support their research.”

Christian Henry, President and CEO of PacBio

Kinnex application kits

Kinnex full-length RNA kit Kinnex single-cell RNA kit Kinnex 16S rRNA kit
Kit advantage Full-length isoform discovery with flexible sample multiplexing Cell type-specific isoform isoform discovery with the highest accuracy Full-length 16S rRNA for species identification
Total throughput

15-20M (Sequel II)
30-40M (Revio)

30-40M (Sequel II)
80-100M (Revio)

20-25M (Sequel II)
50-60M (Revio)

Sample multiplexing recommendation*

5M × 3-plex (Sequel II)
10M × 4-plex (Revio)

1-plex (Sequel II)
1- or 2-plex (Revio)

384-plex (Sequel II)
384- to 1,536-plex (Revio)

Analysis SMRT Link v13.0 and above To be released SMRT Link v13.0 and above

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Read the tertiary tools app note

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Read the tertiary analysis wiki

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Try the 16S analysis workflow

* Sample multiplexing and read depth recommendation will vary by experimental goal, tissue, and species.
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On-demand webinar

Increasing the throughput of full-length 16S and RNA-Seq with Kinnex kits

Explore the transformative capabilities of Kinnex kits from PacBio, in reshaping the landscape of microbiome community profiling and RNA-Seq in plant and animal research. This webinar guides you through the enhanced functionalities of full-length 16S rRNA and RNA-Seq, empowered by the new Kinnex kits (using MAS-Seq technology/method), which facilitate a substantial increase in the number of reads obtained from PacBio long-read sequencing platforms. Delve into detailed customer scientific studies that showcase the practical applications and influential impact of Kinnex kits in contemporary research environments.

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RNA sequencing datasets

Application Dataset Download literature Technology Sequencing system
Kinnex single-cell Homo sapiens - PBMC 10x Chromium Single Cell (5' and 3' libraries) Kinnex single-cell application note HiFi long-read Sequel II and Revio systems
Kinnex single-cell Homo sapiens - HG002 (10x 5') Kinnex single-cell RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens – UHRR Kinnex full-length RNA application note HiFi long-read Sequel II and Revio systems
Kinnex full-length RNA Homo sapiens – HG002 Kinnex full-length RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens - Heart Kinnex full-length RNA application note HiFi long-read Revio system
Kinnex full-length RNA Homo sapiens - Cerebellum Kinnex full-length RNA application note HiFi long-read Revio system
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FEATURED LONG-READ SEQUENCING SYSTEMS

Achieve highly accurate full-length cDNA sequencing with PacBio long-read sequencers.

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