What is Kinnex?
The Kinnex kits are based on the MAS-Seq method, which concatenates smaller amplicons into larger fragment libraries for throughput increase.
Kinnex kits increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio long-read sequencing systems to enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.
Want to watch more Kinnex webinars? Learn from tutorials? See how it’s being used by other researchers? Check out the Kinnex YouTube playlist.
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Kinnex for Human Genomics Research
Long-read RNA neurology symposium
In this virtual symposium, hear from a variety of researchers who are using the PacBio Iso-Seq method for bulk and single-cell transcriptomics to drive cutting-edge research in neurology, neurodegenerative diseases, and brain development.
Advancing Cardiology Research with Long-read RNA Sequencing
In this webinar, learn how long-read transcriptomics at full-length isoform resolution is transforming our understanding of heart development, function, and disease.
Leveraging isoform-level RNA sequencing to understand rare disease pathogenesis
In this webinar, Dr. Carolina Jaramillo Oquendo, a Research Fellow at the University of Southampton, presents her analyses of rare disease samples, in collaboration with the University Hospital Southampton NHS Foundation Trust. Dr. Oquendo also discusses her groundbreaking work demonstrating how transcriptomics, utilizing the new full-length Kinnex RNA kit.
Long-read value for RNA applications
Isoform quantification using deep coverage long-read RNA-Seq
Researchers at ETH Zurich and UVA applied Kinnex full-length RNA-Seq to deeply sequence WTC-11 cell line over multiple timepoints and replicates to create the largest single cell line long-read RNA-Seq dataset to date. They showed Kinnex demonstrates better and consistent isoform detection and quantifiability compared to short reads, especially in cases of complex alternative splicing.
Read the pre-print Watch the webinar
Whitepaper
Full-length bulk and single-cell RNA sequencing for cancer research
The Iso-Seq method (full-length RNA sequencing using PacBio HiFi sequencing) has been shown to advance cancer research by unambiguously characterizing isoforms at both the bulk and single-cell level. PacBio Kinnex RNA kits further increase throughput on HiFi long-read sequencers. This whitepaper highlights publications on cancer research to demonstrate how bulk and single-cell Iso-Seq data detects fusion genes, identifies novel isoforms that might serve as neoepitopes for vaccine candidates, and traces clonal evolution.
Kinnex application kits
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| Kinnex full-length RNA kit | Kinnex single-cell RNA kit | Kinnex 16S rRNA kit | ||||
| Kit advantage | Full-length isoform discovery with flexible sample multiplexing | Cell type-specific isoform isoform discovery with the highest accuracy | Full-length 16S rRNA for species identification | |||
| Total throughput |
15-20M (Sequel II) |
30-40M (Sequel II) |
20-25M (Sequel II) |
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| Sample multiplexing recommendation* |
5M × 3-plex (Sequel II) |
1-plex (Sequel II) |
384-plex (Sequel II) |
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| Analysis | SMRT Link v13.0 and above | SMRT Link v13.1 and above | SMRT Link v13.0 and above | |||
| * Sample multiplexing and read depth recommendation will vary by experimental goal, tissue, and species. | ||||||
Technical note
Concatenating cDNA or gDNA amplicons using Kinnex kits to increase throughput
Kinnex kits can be used as a general concatenation kit for cDNA or gDNA amplicons, which can increase the throughput by 8-, 12-, or 16-fold, and decrease sequencing cost. Read the tech note to see how you can use or modify your amplicon for cost-effective sequencing with Kinnex kits on PacBio’s long read sequencers.
“Long-read RNA sequencing results in more accurate measurements than any obtained from short-read RNA-Seq to determine the presence or absence of specific isoforms.”
Schertzer et al. (2024)
Targeted enrichment for Kinnex full-length RNA kit using IDT xGen HybWash v3 Reagents
The IDT xGen Hybridization and Wash kit v3 (early access) is compatible with the Kinnex full-length RNA kit. Enrich for rare isoforms of target genes and increase sample multiplexing with targeted enrichment.
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On-demand webinar
Leveraging isoform-level RNA sequencing to understand rare disease pathogenesis
See how researchers compared their experience analyzing Kinnex full-length RNA data against other sequencing technologies. Cleaner data – faster path to biological insight
RNA sequencing datasets
FEATURED LONG-READ SEQUENCING SYSTEMS
Achieve highly accurate full-length cDNA sequencing with PacBio long-read sequencers.