Datasets - PacBio - Highly accurate long-read sequencing

WGS Targeted RNA Metagenomics COVID File formats

Whole genome sequencing

Application	Dataset	Data type	PacBio system
Variant detection, assembly, epigenetics	Homo sapiens - GIAB trio HG002-4	HiFi reads	Revio system
Whole genome sequencing	Various plant & animals – Darwin Tree of Life Programme	HiFi reads	Revio system
Assembly (ultra-low DNA input)	Phlebotomus papatasi, Homo sapiens, Drosophila melanogaster	HiFi reads	Sequel II system
Assembly	Food safety and infectious microbes – 96 plex	HiFi reads	Sequel II system

Targeted sequencing

Application	Dataset	Data Type	PacBio System
HiFi Target Enrichment	Twist Alliance Dark Genes Panel	HiFi reads	Revio system
HiFi Target Enrichment	Twist Alliance Dark Genes Panel	HiFi reads	Sequel IIe System
HiFi Target Enrichment	Twist Alliance Long Read PGx Panel	HiFi reads	Sequel IIe System
Amplicon sequencing	CYP2D6 amplicon	HiFi reads	Sequel IIe System

RNA sequencing

Application	Dataset	Data type	PacBio system
MAS-Seq single cell	Homo sapiens - PBMC 10x Chromium Single Cell 3' libraries	HiFi reads	Sequel II and Revio systems
Whole transcriptome	Homo sapiens– brain with Alzheimer’s disease	HiFi reads	Sequel II system
Whole transcriptome	Homo sapiens – universal human reference RNA (UHRR)	HiFi reads	Sequel II system

Metagenomics

Application	Dataset	Data type	PacBio system
Metagenomic profiling and assembly	20 strain mock microbial community – ATCC MSA-1003 – shotgun	HiFi reads	Sequel II system
Metagenomic profiling and assembly	Human gut microbiome pooled standards	HiFi reads	Sequel II system
Full-length 16S sequencing	20 strain mock microbial community – ATCC MSA-1003 – 16S	HiFi reads	Sequel II system

COVID-19 sequencing

Application	Dataset	Data type	PacBio system
SARS-CoV-2 surveillance	Omicron samples	HiFi reads	Sequel II system
SARS-CoV-2 surveillance	Synthetic RNA controls	HiFi reads	Sequel II system
SARS-CoV-2 surveillance	Surveillance samples	HiFi reads	Sequel II system

File formats

Dataset	Data type	PacBio system
reads.bam and hifi_reads.bam	HiFi reads	Sequel IIe system
reads.bam and hifi_reads.bam with kinetic information	HiFi reads	Sequel IIe system

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