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DATASETS

Explore these publicly available datasets generated with PacBio sequencing.

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WGS     Targeted     RNA     Metagenomics    AAV  COVID    File formats

Whole genome sequencing

Application Dataset Download literature Technology Sequencing system
Variant detection, assembly, epigenetics Homo sapiens — GIAB trio HG002-4 N/A HiFi long read Revio system
Tumor/normal COLO829 melanoma N/A HiFi long read Revio system
Tumor/normal HCC1395 N/A HiFi long read Revio system
Whole genome sequencing Various plant & animals – mouse, ladybug, oak, mistletoe, and maize Plant + animal biology HiFi long read Revio system
Whole genome sequencing Homo sapiens — HG002 N/A SBB short read Onso system
Assembly (ultra-low DNA input) Phlebotomus papatasi, Homo sapiens, Drosophila melanogaster Considerations for using ultra-low DNA input workflows for WGS HiFi long read Sequel II system
Assembly Food safety & infectious microbes – 96 plex Microbial WGS HiFi long read Sequel IIe system

Targeted sequencing

Application Dataset Download literature Technology Sequencing System
Target enrichment SeraCare ctDNA w/Agilent capture panel N/A SBB short read Onso system
Target enrichment Twist Alliance Dark Genes Panel HiFi sequencing with Twist Bioscience target enrichment HiFi long read Revio system
Target enrichment Twist Alliance Dark Genes Panel Twist Alliance Dark Genes Panel HiFi long read Sequel IIe system
Target enrichment Twist Alliance Long Read PGx Panel Twist Alliance Dark Genes panel HiFi long read Sequel IIe system
Target enrichment Twist Custom BRCA1/2 Panel HiFi sequencing with Twist Bioscience target enrichment HiFi long read Sequel IIe system
Amplicon sequencing CYP2D6 amplicon HiFi amplicon sequencing for pharmacogenetics: CYP2D6 HiFi long read Sequel IIe system

RNA sequencing

Application Dataset Download literature Technology Sequencing system
MAS-Seq single cell Homo sapiens - PBMC 10x Chromium Single Cell 3' libraries MAS-Seq for single-cell isoform sequencing HiFi long read Sequel II and Revio systems
Whole transcriptome Homo sapiens– brain with Alzheimer’s disease Bulk and single-cell isoform sequencing for human disease research HiFi long read Sequel II system
Whole transcriptome Homo sapiens – universal human reference RNA (UHRR) Bulk and single-cell isoform sequencing for human disease research HiFi long read Sequel II system

Metagenomics

Application Dataset Download literature Technology Sequencing system
Metagenomic profiling and assembly ZymoBIOMICS Fecal Reference with TruMatrix Technology (human) Microbiome and metagenome sequencing with HiFi reads HiFi long read Revio system
Metagenomic profiling and assembly ZymoBIOMICS Fecal Reference with TruMatrix Technology (human) Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel IIe system
Metagenomic profiling and assembly 20 strain mock microbial community – ATCC MSA-1003 – shotgun Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel II system
Metagenomic profiling and assembly Human gut microbiome pooled standards Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel IIe system
Full-length 16S sequencing 20 strain mock microbial community – ATCC MSA-1003 – 16S Microbiome and metagenome sequencing with HiFi reads HiFi long read Sequel II system

Gene therapy vectors

Application Dataset Download literature Technology Sequencing system
AAV scAAV 8-plex – Vector Biolabs rh10-CBA-eGFP AAV sequencing with HiFi reads HiFi long read Sequel II system
AAV ssAAV – ViGene pAV-CMF-GFP AAV sequencing with HiFi reads HiFi long read Sequel II system
AAV ssAAV-scAAV 1:10 mixture AAV sequencing with HiFi reads HiFi long read Sequel II system

COVID-19 sequencing

Application Dataset Data type PacBio system
SARS-CoV-2 surveillance Omicron Samples HiFi reads Sequel II system
SARS-CoV-2 surveillance Synthetic RNA controls HiFi reads Sequel II system
SARS-CoV-2 surveillance Surveillance samples HiFi reads Sequel II system

File formats

Dataset Data type PacBio system
8 SMRT Cell run directory Directory Revio system
reads.bam and hifi_reads.bam HiFi reads Sequel IIe system
reads.bam and hifi_reads.bam with kinetic information HiFi reads Sequel IIe system

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