See what is possible
Single Molecule, Real-Time (SMRT) sequencing on the PacBio systems provides a deeper understanding of microbes and microbial communities by giving scientists the ability to:
- Resolve complete microbial genomes and detail their mobile elements
- Characterize epigenetic modifications and their impact on critical bacterial traits
- Investigate viral pathogenicity, evolution, and selection
- Understand microbial communities
Precise species profiling, more functional annotations, more high-quality MAGs
The authors concluded that [PacBio] long-read approaches provide valuable additional information including greater taxonomic resolution with long-read amplicon sequencing and improved functional profiling and bacterial genome recovery from long-read metagenomics. Explore this research further:
Gehrig, J., et al. (2022) Finding the right fit: evaluation of short-read and long-read sequencing approaches to maximize the utility of clinical microbiome data. Microbial Genomics, 8(3), 10.1099/mgen.0.000794.
Improved genome assembly corrects understanding of virulence
SMRT sequencing corrected errors in the Mycobacterium tuberculosis H37Rv reference genome originally produced using Sanger sequencing. The improved reference genome revealed important variant strain differences that affect virulence. Explore this research further:
Elghraoui, A., Modlin, S.J. & Valafar, F., 2017. SMRT genome assembly corrects reference errors, resolving the genetic basis of virulence in Mycobacterium tuberculosis. BMC Genomics, 18(1), p.302.