Microbial sequencing methods

Advancing our understanding of the microbial world through discovery-based research entails comprehensive analysis of microbial genomes, their interactions, communities, and environments in which they live.

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Single Molecule, Real-Time (SMRT) sequencing on the PacBio systems provides a deeper understanding of microbes and microbial communities by giving scientists the ability to:

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A new standard in pathogen genomics

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

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Precise species profiling, more functional annotations, more high-quality MAGs

The authors concluded that [PacBio] long-read approaches provide valuable additional information including greater taxonomic resolution with long-read amplicon sequencing and improved functional profiling and bacterial genome recovery from long-read metagenomics. Explore this research further:

Gehrig, J., et al. (2022) Finding the right fit: evaluation of short-read and long-read sequencing approaches to maximize the utility of clinical microbiome data. Microbial Genomics, 8(3), 10.1099/mgen.0.000794.


Improved genome assembly corrects understanding of virulence

SMRT sequencing corrected errors in the Mycobacterium tuberculosis H37Rv reference genome originally produced using Sanger sequencing. The improved reference genome revealed important variant strain differences that affect virulence. Explore this research further:

Elghraoui, A., Modlin, S.J. & Valafar, F., 2017. SMRT genome assembly corrects reference errors, resolving the genetic basis of virulence in Mycobacterium tuberculosisBMC Genomics, 18(1), p.302.

Pink and blue Roundel for SMRT genome assembly corrects reference errors, resolving the genetic basis of virulence in Mycobacterium tuberculosis

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