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Resolving complexity

Generating new insights into how microbial communities function requires improved approaches. Long-read sequencing enables species-level resolution and drives functional insights with full-length 16S rDNA and shotgun sequencing for metagenomic profiling and assemblies.

See the microbial world in high resolution

Single Molecule, Real-Time (SMRT) Sequencing for metagenomics pairs long reads with high accuracy, enabling you to:

  • Understand key community functions by sequencing complete operons at 99.9% accuracy with long-insert metagenomic profiling – no assembly required
  • Identify microbial community members with strain-level resolution using full-length 16S rDNA or shotgun sequencing
  • Discover novel genes and gene clusters by reconstructing multi-kilobase long contigs with whole genome shotgun metagenomic assembly to discover novel genes and gene clusters
  • Leverage epigenomic data to associate contigs and plasmids from closely related strains

Workflows: from DNA to resolved microbial communities

 
Library Preparation

 

 

SMRT Sequencing with the Sequel System

  • Achieve ~15 kb average read lengths, with some reads as long as 100 kb
  • Over 250,000 reads per SMRT Cell at QV30 to resolve 16S rDNA sequences
  • 50-fold coverage per genome recommended for de novometagenomic assembly
 

 

Data Analysis through SMRT Analysis, PacBio DevNet or Service Partners

Spotlight: Accurately mapping mycobiota to understand human health

Scientists explored various sequencing techniques to establish the most rigorous method to map human gut mycobiota of healthy individuals. Their results showed that PacBio SMRT Sequencing most accurately represented the metagenomic population profile. Explore this research further:

Motooka, D. et al., 2017. Fungal ITS1 deep-sequencing strategies to reconstruct the composition of a 26-species community and evaluation of the gut mycobiota of healthy Japanese individuals. Frontiers in Microbiology, 8, p.238.

To learn more about how to fully resolve your microbial community, contact us.

Selected Resources