Revio system
Most affordable, complete, phased genome
HiFi reads combine long-read length with high accuracy, replacing multiple assays and confidently resolving complex regions beyond the limits of short reads and nanopore methods. ~$345* human genome at 20x coverage with SPRQ-Nx. List pricing in USD. Regional pricing may differ. Contact your PacBio sales rep.
HiFi sequencing at scale, with less DNA input
Built for population-scale studies and programs that don’t pause. Run up to ~2,500 human whole genomes per year, up to 480 Gb of HiFi reads per day, and up to 4 SMRT Cells simultaneously with configurable run times and flexible scheduling — so sequencing keeps pace with your science.
On-instrument multiomic insight, included
Every standard run delivers on-instrument 5mC, 5hmC, and 6mA methylation calling—at no additional cost or bisulfite treatment—giving you access to genomic and epigenetic data from a single sample.
Confidence in every run, without the complexity
HiFi accuracy powered by SPRQ-Nx chemistry and Google Research DeepConsensus, paired with an easy, automation-ready workflow supported by compatible partners—so you get reliable results with minimal hands-on time.
SPRQ-NX chemistry - Available now
The Revio system runs on SPRQ chemistry and helps you achieve more with less DNA, empowering you to make more discoveries faster and more affordably. New SPRQ-Nx extends SPRQ chemistry with improved consistency, a new 5hmC methylation caller, and multi-use SMRT Cells, enabling a richer and cost-effective HiFi workflow. Built for projects that scale, it maintains the gold-standard HiFi quality you rely on, from population studies to routine panels.
Better economics with same quality
~30% LOWER COST PER GENOME WITH SPRQ-NX
Per genome cost at 20x coverage based on 2026 reagent list price*.
*At scale pricing with volume discounts can reach ~$300 per genome. Contact your PacBio sales rep. Study design, sample type, and level of multiplexing may affect the number of SMRT Cells required. All prices are listed in USD and costs may vary by region. Pricing includes sequencing reagents run on your system and does not include instrument amortization or other reagents.
Compare with other technologies
POPULATION-SCALE GENOMES WITHOUT THE PRICE TAG
Total sequencing reagent cost for 10,000 human WGS at 20x. Access the app brief to learn more.
*Sequencing reagent cost only. Excludes library prep, instrument capital, labor, sequencing-core overhead, and downstream data storage/compute.
POPULATION-SCALE STUDIES
Estonia National Biobank selects PacBio to sequence 10,000 whole genomes
HiFi data generated on the Revio system will support the EU and Estonian government-funded Center for Personalized Medicine to enable discoveries across cardiology, mental and reproductive health, drug response, cancer research and rare diseases.
PacBio and GeneDx Launch Research Collaboration with the University of Washington
This study is first of its kind to compare diagnostic rates across short- and long-read sequencing platforms to understand the potential for increased diagnostic yield in neonatal care.
Ambry Genetics and PacBio Announce Collaboration to Sequence Up to 7,000 Human Genomes
Consortium selects leading genomics companies to support pediatric Mendelian Genomics Research Center Program aimed at providing insights for families battling rare diseases.
SPRQ-NX: HEAR FROM YOUR PEERS
Expanded multiomic insight
SPRQ-Nx adds 5hmC detection and strengthens 5mC and 6mA methylation calling with no additional prep.
Key applications and sample throughput with SPRQ-Nx chemistry
“HiFi long read sequencing can now produce the most comprehensive variant dataset obtainable by a single technology in a single laboratory, allowing accurate calling of substitutions, indels, STRs and SVs.”
– Kucuk et al., Genome Med, 2023
More HiFi reads, more samples, more discovery
System specifications with SPRQ-Nx chemistry
The Revio system utilizes nanofabricated Revio SMRT Cells and has four independent stages, allowing sequencing of multiple SMRT Cells in parallel. The onboard compute provides accurate basecalling with Google DeepConsensus, plus methylation calling in every run.
| Library | Run timea | Q30+ bases | HiFi yield per SMRT Cellb | Methylation | ||||
|---|---|---|---|---|---|---|---|---|
| 0.5-5 kbd | 12 hours | 95% | 6-8 million reads | 5mC and 5hmC at CpG sites and 6mA for native DNAc | ||||
| 5-10 kb | 24 hours | 90% | 35-70 Gb | |||||
| 10-15 kb | 70-100 Gb | |||||||
| 15-20 kb | 100-120 Gb | |||||||
| 20-25 kb | 30 hours | 85% | 100-120 Gb | |||||
| ||||||||
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A WORKFLOW BUILT FOR YOUR SCHEDULE
The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:
Fast, reliable library preparation
Library prep for the Revio system supports manual and automated prep. Learn more
Rapid run setup
Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.
Flexibility to fit your schedule
The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.
Multi-use workflow efficiency
SMRT Cells are automatically washed, handled, and tracked on-instrument between uses, reducing manual intervention and downtime.
A smarter workflow with a lighter footprint
Reduce waste and increase productivity with automated consumable reuse designed to deliver more HiFi data while simplifying your workflow.
SPRQ-Nx Chemistry: Now Available
Reduce waste and increase productivity with automated consumable reuse designed to deliver more HiFi data while simplifying your workflow.
Consistent trusted HiFi performance
The Revio system with SPRQ-Nx chemistry produces exceptional read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.
Reveal a more complete genome
The complete, gapless human genome marks a new era of genomics where no region of the genome is beyond your reach. In 2021, PacBio HiFi technology was used by the Telomere-to- Telomere (T2T) Consortium to fill in those gaps.
Read length
HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.
Read and base quality
The Revio system provides reliable answers through exceptional accuracy, with 90% of bases Q30+ and median read accuracy ≥Q30.
Variant calling
HiFi sequencing with SPRQ-Nx chemistry on the Revio system delivers high accuracy variant detection, resulting in fewer sequencing errors relative to other technologies at lower coverage1.
How HiFi long-read sequencing compares
| PacBio HiFi sequencing | SBS sequencing | Nanopore sequencing | |
|---|---|---|---|
| Read length | 15-20 kb5 | 2x150 bp | 10–100 kb |
| Read accuracy | 99.95% (Q33)1 | 99.92% (Q31)3 | 98.90% (Q19)4 |
| Run time | 24 hours2 | 44 hours | 72 hours |
| Yield | 120 - 480 Gb2 | 2,400–3,000 Gb | 50–110 Gb |
| Coverage | Unbiased | Reduced at low and high (GC) | Reduced in low-complexity runs |
| Genome completeness6 | ✓ | X | ✓ |
| Variant calling – SNVs, Indels, SVs5 | ✓ | X | X |
| Areas of high homology | ✓ | X | X |
| Phasing | ✓ | X | ✓ |
| Methylation | Every run | Via library prep | Every run |
| 1. Read accuracy PacBio HiFi: precisionFDA Truth Challenge V2 3. Illumina: HG002 2×150 bp NovaSeq library, precisionFDA Truth Challenge V2 | |||
MASSIVE COMPUTE POWER
The Revio system includes powerful NVIDIA GPUs to deliver accurate, ready-to-use data directly from the instrument.
HiFi read generation
The GPUs provide rapid turnaround time for basecalling and HiFi read generation, directly outputting HiFi reads in BAM format.
Google Research DeepConsensus
DeepConsensus combines deep learning with innovations from AlphaEvolve, Google’s AI coding agent, to extend the accuracy and yield of HiFi sequencing.
Methylation calling (5mC, 5hmC, and 6mA)
Standard runs include DNA methylation status, calculated with a deep learning algorithm that processes polymerase kinetics.
Demultiplexing
All fundamental processing steps are performed on instrument, including barcode demultiplexing.
HiFi Workflow
Extraction
Nanobind DNA kits enable extraction of HMW gDNA from a variety of samples: saliva, cultured cells, bacteria, whole blood, tissue, plant nuclei, and insect.
Library prep
SMRTbell prep kit 3.0, HiFi prep kits, and application kits (Kinnex, PureTarget) support manual and automated library prep for effortless SMRTbell library creation.
Sequencing
Built for scale and flexibility, Revio lets you run 4 SMRT Cells at once, and scheduling that fits your lab.
Data analysis
Advanced algorithms from Google Research and cutting-edge NVIDIA GPUs allow for on-board processing of sequencing data, all with reduced output file sizes.
Instrument specifications
| Operating environment | |
| Temperature | 19–25°C (66–77°F) |
| Humidity | 20–80% relative humidity, non-condensing |
| Ventilation | 13,000 BTU/hr (3,800 W) |
| Dimensions | |
| W × D × H | 92.7 cm (36.5 in) × 91.4 cm (36.0 in) × 174.5 cm (68.7 in) |
| Weight | 465 kg (1,025 lb) |
| Floor space | 243.8 cm (96.0 in) × 138.5 cm (54.5 in) |
| Electrical power | |
| Power requirements | 200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A |
| Compute | |
| Network connection | 1 GbE or 10 GbE, copper |
| Output file format | hifi_reads.bam, ~60 GB each, up to 78 TB per year |
Frequently asked questions
HiFi sequencing is a single-molecule long-read technology that produces highly accurate, long DNA reads by combining multiple passes of the same molecule into a consensus sequence, allowing you to resolve complex genomic regions and close gaps in your data.
HiFi sequencing delivers high-accuracy long reads with 99.95% read accuracy (Q33), compared to 99.92% (Q31) for short-read sequencing. HiFi enables accurate detection of SNVs, indels, and structural variants, including in difficult-to-map regions of the genome, along with native phasing and methylation detection.
SPRQ-Nx chemistry delivers the most affordable long-read genome, at approximately ~$345 per human genome at 20x coverage (~$300 at scale), representing about 30% reagent cost savings compared to previous chemistry.
A single SPRQ-Nx acquisition can generate:
- Up to 120 Gb of total HiFi long-read sequencing data in 24 hours.
- Two phased 20x human whole genome.
- One sample with ~80 million full-length RNA isoforms using the Kinnex single-cell RNA kit.
- 16 samples using large, targeted gene panels such as the Dark Gene.
- 96 samples using small, targeted gene panels similar to the Twist Alliance Long-read PGx.
- 16 communities of metagenome assembled genomes.
Expected coverage and throughput are estimates. Coverage and total reads/data may vary based on library quality and fragment lengths.
SPRQ-Nx is an updated HiFi sequencing chemistry for the Revio system that enables multi-use SMRT Cells, reducing cost per human genome to $345 at 20x coverage. It adds 5hmC methylation detection, improves 5mC and 6mA detection performance, and delivers more consistent yield and accuracy across runs, all with the same workflow.
The Revio system outputs ready-to-use HiFi reads in BAM format. All fundamental processing steps are performed on instrument, including barcode demultiplexing.
Learn more
Revio system resources
SPRQ-Nx app brief
SPRQ-Nx scaling webinar
How to set up a run on Revio
Commonly asked questions about HiFi
Public benchmarks
Revio system documentation
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Service plans + support