Revio system
UNLEASH THE POWER OF HIFI SEQUENCING
The Revio system adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.
15× increase in HiFi read throughput
With a high-density, 25 million ZMW SMRT Cell, up to 4 SMRT Cells in parallel, and 24-hour run times, the Revio system delivers 360 Gb of HiFi reads per day, equivalent to 1,300 human whole genomes per year.
The $1,000 complete, phased genome
HiFi sequencing provides small variants, structural variants, repeat expansions, methylation, and haplotype phasing from a single library and sequencing run.
50% fewer consumables and a vastly simplified experience
Operating the Revio system is simple, thanks to a combined reagent and sample plate, rapid run setup, flexibility to queue runs while sequencing is in progress, and no external nitrogen supply.
Google Health DeepConsensus on board
Advanced algorithms on the Revio system deliver exceptional read accuracy plus methylation calling in every run. Its optimized file formats reduce data storage needs through quality value binning and smart read ordering.
HiFi sequencing at scale
With the Revio system, you can achieve higher throughput to support large studies and develop novel applications. The Revio system can generate 1,300 human genome equivalents per year, 15× more than the Sequel IIe system, via:
| Revio system | Sequel IIe system | Increase | |
|---|---|---|---|
| Higher density | 25 million ZMW | 8 million ZMW | 3× |
| Independent stages | 4 | 1 | 4× |
| Shorter run times | 24 hrs | 30 hrs | 1.25× |
| 15× |
Simplified Consumables
The Revio system simplifies the HiFi sequencing experience with fewer consumables optimized for easy tracking and minimal waste. With workflow improvements, loading consumables takes under a minute at the instrument.
Revio polymerase kit
The polymerase kit contains optimized reagents to bind polymerase to 24 SMRTbell libraries in preparation for sequencing.
Revio SMRT Cell tray
One tray contains 4 high-density SMRT Cells, each with 25 million zero-mode waveguides.
Revio sequencing plate
The sequencing plate provides reagents for sequencing up to 4 Revio SMRT Cells and combines multiple parts from the Sequel IIe system: sequencing plate, sample plate, mixing plate, and SMRT Cell oil.
A WORKFLOW BUILT FOR YOUR SCHEDULE
The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:
Fast, reliable library preparation
Library prep for the Revio system supports manual and automated preps with SMRTbell prep kit 3.0.
Rapid run setup
Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.
Flexibility to fit your schedule
The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.
MASSIVE COMPUTE POWER
The Revio system includes powerful NVIDIA GPUs, which provide more than 20× the compute power of the Sequel IIe system, to deliver accurate, ready-to-use data directly from the instrument.
HiFi read generation
The GPUs provide rapid turnaround time for basecalling and HiFi read generation, directly outputting HiFi reads in BAM format.
Google Health DeepConsensus
DeepConsensus uses advanced deep learning techniques to further extend the accuracy and yield of HiFi sequencing.
Methylation calling
Standard runs include DNA methylation status, calculated with a deep learning algorithm that processes polymerase kinetics.
Demultiplexing
All fundamental processing steps are performed on instrument, including barcode demultiplexing.
HIFI LEVEL ACCURACY AND DATA QUALITY
The Revio system produces the same — and better — read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.
Read length
HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.
Read and base quality
The Revio system provides reliable answers through exceptional accuracy, with 90% of bases ≥Q30 and median read accuracy ≥Q30.
Variant calling
HiFi sequencing on the Revio system delivers high accuracy for all variants types, matching the precisionFDA-winning accuracy of the Sequel IIe system.1
“…the high-throughput capabilities of the Revio system will allow us to apply HiFi technology to a wider array of applications in agricultural biotechnology“
— Gina Zastrow-Hayes, Biotechnology and GT-Genomics Technology Manager at Corteva Agriscience
How Revio long-read sequencing compares
| PacBio Revio | SBS sequencing | Nanopore sequencing | |
|---|---|---|---|
| Read length | 15–18 kb | 2x150 bp | 10–100 kb |
| Read accuracy | 99.95% (Q33) | 99.92% (Q31) | 99.26% (Q21) |
| Run time | 24 hours | 44 hours | 72 hours |
| Yield | 90 Gb | 2,400–3,000 Gb | 50–110 Gb |
| Variant calling — SNVs |
✓ |
✓ |
✓ |
| Variant calling — indels |
✓ |
✓ |
X |
| Variant calling — SVs |
✓ |
X |
✓ |
| 5mC methylation |
✓ |
X |
✓ |
| Phasing |
✓ |
X |
✓ |
Workflow
Library prep
SMRTbell prep kit 3.0 supports manual and automated library prep for effortless SMRTbell library creation.
Sequencing
The Revio system offers simultaneous acquisition of 4 SMRT Cells on a 24-hour run, with flexible run scheduling to fit your schedule.
Data analysis
Advanced algorithms from Google Health and cutting-edge NVIDIA GPUs allow for on-board processing of sequencing data, all with reduced output file sizes.
More HiFi reads, more samples, more discovery
Sequencing specifications
|
HiFi yield2 |
|||||
|---|---|---|---|---|---|
| Library |
Run time3 |
1 SMRT Cell | 4 SMRT Cells | Base quality | Methylation |
| 15–18 kb | 24 hours | 90 Gb | 360 Gb | 90% Q30+ | 5mC at CpG sites |
Application performance
HiFi reads enable a wide variety of applications with different coverage, read length, and accuracy requirements. Based on typical yields and coverage requirements, the performance for select popular applications is:
| Throughput | |||
|---|---|---|---|
| Application | Per Revio SMRT Cell |
Per year4 |
Expected coverage5 |
| Human genome | 1 | 1,300 | 30× |
| Single-cell transcriptome | 1 | 1,300 | 80 million reads |
| Large gene panel (20 Mb) | 12 | 15,600 | 90% target positions ≥10× |
Instrument specifications
| Operating environment | |
| Temperature | 19–25°C (66–77°F) |
| Humidity | 20–80% relative humidity, non-condensing |
| Ventilation | 13,000 BTU/hr (3,800 W) |
| Dimensions | |
| W × D × H | 92.7 cm (36.5 in) × 91.4 cm (36.0 in) × 174.5 cm (68.7 in) |
| Weight | 465 kg (1,025 lb) |
| Floor space | 243.8 cm (96.0 in) × 138.5 cm (54.5 in) |
| Electrical power | |
| Power requirements | 200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A |
| Compute | |
| Network connection | 1 GbE or 10 GbE, copper |
| Output file format | hifi_reads.bam, ≈55 GB each, up to 72 TB per year |
Download spec sheet Access data sets
Frequently asked questions
The Revio system is available to order now. The first systems will ship to customers in the first quarter of 2023. We recommend placing orders now to ensure earliest delivery.
U.S. list price is $995 for sequencing reagents for one Revio SMRT Cell, which has an expected yield of 90 Gb, equivalent to a 30X human genome. Your local sales representative can provide detailed pricing in your currency.
Yes. The same library prep protocols are available for the Sequel systems and the Revio system. Therefore, SMRTbell libraries can be used across systems. However, the sequencing reagents — polymerase/binding kit, sequencing plate, and SMRT Cell tray — are specific to each system.
Yes. The hifi_reads.bam format continues to adhere to the SAM specification. The Revio BAM format has minor differences – for data size efficiency – in base quality encoding and read sort order.
- Data shown is for a single Revio SMRT Cell for HG002/GM24385.
- HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0.
- Run time specification is for the sequencing reaction.
- Annual throughput is based on 1,300 Revio SMRT Cells.
- Expected coverages and throughputs are estimates. Coverage may vary based on library quality and fragment lengths.