Featured Documentation

Operations guide – Revio system

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT® Link (v12.0) user guide.

SMRT Link Cloud Reference Guide v12.0

Description

This document is for Customer IT or SMRT Link administrators, and describes the procedures for installing and using SMRT Link Cloud on Amazon AWS services. It also documents the command-line utilities provided by PacBio for use with SMRT Link Cloud, and includes a Frequently Asked Questions section.

SMRT Link Installation v12.0

Description

This document describes the procedure for installing SMRT Link v12.0. This document is for Customer IT or SMRT Link administrators. SMRT Link v12.0 supports Revio^TM systems, Sequel® II systems and Sequel IIe systems. Sequel systems are not supported.

SMRT Link Release Notes v12.0

SMRT Link User Guide v12.0

Description

This document describes how to use PacBio’s SMRT Link software. SMRT Link is the web-based end-to-end workflow manager for Revio^TM, Sequel® II, and Sequel IIe systems.

SMRT Link web services API use cases v12.0

Description

This document describes common tasks performed using the SMRT Link web services API and provides "how to" recipes for accomplishing these tasks.

SMRT Tools Reference Guide v12.0

Description

This document describes the command-line tools included with SMRT® Link v12.0. These tools are for use by bioinformaticians working with secondary analysis results.

Overview – Nanobind HMW DNA extraction overview

Description

This overview provides links to all standard procedures for extracting HMW DNA by sample type and compatible Nanobind kit.

Procedure checklist preparing MAS-Seq libraries using MAS-Seq for 10x 3 concatenation kit

Description

This procedure describes the workflow for constructing single-cell MAS-Seq (Multiplexed Arrays Sequencing) libraries from 10x Chromium 3’ cDNA using the MAS-Seq for 10x Single Cell 3’ kit for library prep and sequencing on PacBio systems.

SMRT Link installation guide v11.1

Description

This document describes the procedure for installing SMRT Link v11.1 on a customer system. This document is for use by Customer IT.

SMRT Link release notes v11.1

Description

Release notes for SMRT Link v11.1.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Guide & overview – Short Read Eliminator (SRE) XS and XL kits

Description

This document describes procedures on how to use the the SRE kit family (SRE XS, SRE XL) for rapid high-pass size selection of high molecular weight (HMW) DNA samples.

Overview – Sequel Systems Application Options and Sequencing Recommendations

Description

This document provides high-level application workflow guidance for preparing sample, sequencing on the Sequel or Sequel II System, and analysis.