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Documentation + protocols

Welcome to the PacBio Documentation page. The table below allows you to search for and download the latest guides, protocols, product information, and more. For any questions about our documentation, please contact us.

April 22, 2022

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System
  • Template Preparation

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
April 21, 2022

SDS – SMRTbell cleanup beads

  • SDS
  • Sequel II System:Sequel IIe System, Sequel System
  • Binding, Template Preparation

SDS SMRTbell Cleanup Beads
April 20, 2022

Sequel SMRT Link web services API use cases (v11.0)

  • Developer Documentation
  • Sequel II System, Sequel IIe System
  • Analysis

This document describes common tasks performed using the SMRT Link (v11.0) web services API and provides "how to" recipes for accomplishing these tasks.
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