Documentation
Welcome to the PacBio Documentation page. The table below allows you to search for and download the latest guides, protocols, product information, and more. For any questions about our documentation, please contact us.
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Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons
Sequel II System, Sequel IIe System, Sequel System
Template Preparation
Description +
The procedure describes a workflow for quickly constructing SMRTbell libraries from PCR products using PacBio Barcoded Overhang Adapters and SMRTbell Express Template Prep Kit 2.0. In this workflow, a barcode is introduced to each amplicon through ligation with a hairpin adapter containing a 16-bp barcode. Once barcoded, amplicons can be pooled and purified for sequencing.
Reference Guide – Human Leukocyte Antigen (HLA) Sequencing
Sequel II System, Sequel IIe System, Sequel System
Description +
This reference guide describes recommended procedures for sequencing HLA Class I and Class II genes for producing fully-phased, unambiguous, allele-level information
Procedure & Checklist – Amplification of Full-Length 16S Gene with Barcoded Primers for Multiplexed SMRTbell Library Preparation and Sequencing
Sequel II System, Sequel IIe System, Sequel System
Template Preparation
Description +
This document contains instructions for amplifying and constructing a SMRTbell library using Express 2.0 for sequencing full-length 16S genes on the Sequel Systems.
Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded M13 Primers for Multiplex SMRT Sequencing
Sequel II System, Sequel IIe System, Sequel System
Description +
This procedure can be used to generate asymmetrically barcoded PCR products for library construction and SMRT sequencing by amplifying the region of interest using target-specific PCR primers tailed with M13 forward and reverse primers.
Procedure & Checklist – No-Amp Targeted Sequencing Utilizing the CRISPR-Cas9 System
Sequel II System, Sequel IIe System, Sequel System
Binding, Sequencing, Template Preparation
Description +
This procedure can be used to sequence targeted genomic regions, without amplification, using a CRISPR-Cas9 system. This protocol is applicable for the Sequel and Sequel II Systems.
Overview – Sequel Systems Application Options and Sequencing Recommendations
Sequel II System, Sequel System
Analysis, Binding, Sequencing, Template Preparation
Description +
This document provides high-level application workflow guidance for preparing sample, sequencing on the Sequel or Sequel II System, and analysis.
Quick Reference Card – Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems
Sequel II System, Sequel IIe System
Binding, Sequencing, Template Preparation
Description +
This document contains loading and pre-extension recommendations for the Sequel II System.
Operations Guide – Sequel II and Sequel IIe Systems: The SMRT Sequencers
Sequel II System, Sequel IIe System
Sequencing
Description +
This document describes operational procedures for Sequel II and Sequel IIe Systems. (PN: 101-774-700)
Quick Reference Card – Loading and Pre-Extension Recommendations for the Sequel System
Sequel System
Binding, Sequencing, Template Preparation
Description +
This document contains loading and pre-extension recommendations for the Sequel System.
Guide – Sequel II and Sequel IIe System Safety
Sequel II System, Sequel IIe System
Sequencing
Description +
This guide provides important safety information on the Sequel II and Sequel IIe Systems from Pacific Biosciences. It also provides product compliance and regulatory statements. Read this document before performing any procedures on the Sequel II or Sequel IIe System.
Insert – AMPure PB
PacBio RS II, Sequel II System, Sequel System
Template Preparation
Description +
Package insert for AMPure PB beads (PN: 100-265-900).
Insert – Elution Buffer 50 mL
Sequel II System, Sequel System
Template Preparation
Description +
Package insert for Elution Buffer 50 mL (PN: 101-708-100)
Quick Reference Card – Equipment and Materials Needed for Sequencing
Sequel System
Binding, Sequencing, Template Preparation
Description +
This Quick Reference Card lists examples of equipment and other lab materials that may be needed for sequencing on PacBio instruments.
SDS – SMRTbell gDNA Sample Amplification Kit
Sequel II System, Sequel System
Sequencing
Description +
Safety data sheet for the SMRTbell gDNA Sample Amplification Kit
Insert -SMRTbell gDNA Sample Amplification Kit
Sequel II System, Sequel System
Sequencing
Description +
Package insert for SMRTbell gDNA Amplification Kit