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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols, and product information. See Other Documentation for more specialized and older documentation.

October 20, 2022

SMRT Link installation guide v11.1

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Analysis

Description

This document describes the procedure for installing SMRT Link v11.1 on a customer system. This document is for use by Customer IT.
October 20, 2022

SMRT Tools reference guide v11.1

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Analysis

Description

This document describes the command-line tools included with SMRT Link v11.1. These tools are used by bioinformaticians working with secondary analysis results.
November 4, 2022

SMRT Link user guide v11.1

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Analysis

Description

This document describes how to use PacBio's SMRT Link software (v11.1). SMRT Link is the web-based end-to-end workflow manager for Sequel II/IIe systems.
July 27, 2022

Guide & overview – Nanobind plant nuclei kit

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This document describes procedures on how to use the Nanobind Plant Nuclei Big DNA Kit for extraction of HMW (50 kb – 300+ kb) DNA from plant nuclei.
July 27, 2022

Guide & overview – Nanobind CBB kit

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.
August 26, 2022

Guide & overview – Nanobind tissue kit

  • Guide & Overview
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This document describes procedures on how to use the Nanobind tissue kit for extraction of HMW (50 kb – 300+ kb) and UHMW (50 kb – 1+ Mb) DNA from animal tissue samples.
October 8, 2022

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System
  • Library Preparation

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
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