Documentation + protocols

This document provides a quick step-by-step workflow (from DNA Repair and A-tailing to AMPure PB size selection) for preparing SMRTbell sequencing libraries using SMRTbell prep kit 3.0.

This document describes the PCR amplification of bacterial full-length 16S rRNA genes (V1—V9 regions) using up to 192 dual indices.

This procedure can be used to construct Iso-Seq libraries from RNA for sequencing on PacBio Sequel II and IIe systems.

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

SDS SMRTbell Cleanup Beads

SDS - Sequel II Binding Kit 3.2

SDS - Sequel Binding Kit 3.1

SDS - Low-TE Buffer pH 8.0

SDS Sequel II sequencing kit 2.0 (1-rxn)

Insert - Sequel II binding kit 3.1

Insert - SMRTbell cleanup beads

Insert - Sequel II binding kit 3.2

Release notes for SMRT Link v11.0.

This document describes common tasks performed using the SMRT Link (v11.0) web services API and provides "how to" recipes for accomplishing these tasks.