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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation.

December 12, 2024

Operations guide — Revio system

  • Guide
  • Revio system
  • Sequencing

Description

This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link user guide.
July 1, 2025

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
July 1, 2025

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Vega system, Revio system
  • Extraction, Automation, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
July 1, 2025

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Hamilton NIMBUS Presto robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
July 1, 2025

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non-human mammalian blood NHMB on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50-300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
July 1, 2025

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
May 31, 2025

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system

  • Guide & overview
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Automation, Library preparation

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.
May 31, 2025

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

  • Guide & overview
  • Vega system, Revio system, Sequel II system and Sequel IIe system
  • Automation, Library preparation

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.
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