November 6, 2024
Guide — Vega system site preparation
- Guide, Site preparation
- Vega system
- Sequencing
Featured Documentation
This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation.
May 8, 2024
Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0
- Procedure & checklist
- Revio system, Sequel II system and Sequel IIe system
- Library preparation
Description
This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.
April 30, 2024
Operations guide — Revio system
- Guide
- Revio system
- Sequencing
Description
This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link (v12.0) user guide.
April 5, 2024
SMRT Link User Guide v13.1
- Guide
- Sequel II system and Sequel IIe system, Revio system
- Sequencing
Description
This document describes how to use SMRT Link software from PacBio. SMRT Link is the web-based end-to-end workflow manager for Revio, Sequel II, and Sequel IIe systems.
August 8, 2023
Overview — HiFi application options
- Overview
- Sequel IIe system, Revio system
- Sequencing
Description
Overview of HiFi application options
November 11, 2024
Quick reference card — Onso sample sheet
- Quick reference card
- Onso system
- Sequencing
Description
Quick reference card - Onso sample sheet
November 11, 2024
Quick reference card — Onso system library preparation adapters
- Quick reference card
- Onso system
- Library preparation
Description
Onso indexed adapter sequences are shown here and can be purchased to prepare libraries from genomic, fragmented, or existing P5/P7 libraries. See the appropriate procedures and user guide for more information.
November 11, 2024
SMRT Link Kinnex full-length RNA troubleshooting guide
- Guide, Troubleshooting guide
- Sequel II system and Sequel IIe system, Revio system, Vega system
- Library preparation
Description
SMRT Link Kinnex full-length RNA troubleshooting guide
November 6, 2024
Guide — Revio system site preparation
- Guide, Site preparation
- Revio system
- Sequencing
Description
Site preparation guide for the Revio system
October 29, 2024
Guide & overview — Nanobind CBB kit
- Guide & overview
- Sequel II system and Sequel IIe system, Revio system
- Sample preparation
Description
This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.
October 29, 2024
Guide & overview — Nanobind PanDNA kit
- Guide & overview
- Revio system, Sequel II system and Sequel IIe system
- Extraction, Sample preparation
Description
This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.
October 29, 2024
Overview — Nanobind CBB HMW DNA extraction protocols
- Overview
- Sequel II system and Sequel IIe system, Revio system
- Extraction, Sample preparation
Description
Overview – PacBio Nanobind CBB HMW DNA extraction protocols
October 29, 2024
Overview — Nanobind PanDNA HMW DNA extraction protocols
- Overview
- Sequel II system and Sequel IIe system, Revio system
- Sample preparation
Description
Overview – PacBio Nanobind PanDNA HMW DNA extraction protocols
October 29, 2024
Procedure & checklist — Extracting HMW DNA from saliva collected in DNA Genotek Oragene devices using Nanobind kits
- Procedure & checklist
- Sequel II system and Sequel IIe system, Revio system
- Extraction, Sample preparation
Description
This protocol describes the extraction of HMW DNA (50–300+ kb) from saliva collected with a DNA Genotek Oragene•DNA saliva collection device using Nanobind kits.
October 14, 2024
Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons
- Procedure & checklist
- Sequel II system, Sequel IIe system, Revio system
- Library preparation, Barcoding, Sequencing