April 24, 2023
SMRT Link User Guide v12.0
- Informational Guide
- Sequel II system and Sequel IIe system, Revio system
- Sequencing, Analysis
Featured Documentation
This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. See Other Documentation for product inserts, safety data sheets (SDS) and more.
April 18, 2023
SMRT Link MAS-Seq troubleshooting guide v12.0
- Troubleshooting guide
- Sequel II system and Sequel IIe system, Revio system
- Sequencing, Analysis
Description
This document describes the metrics generated by Read Segmentation and Single-Cell Iso-Seq® workflow in SMRT Link v12.0
March 31, 2023
SMRT Link Installation v12.0
- Informational Guides
- Revio system, Sequel II system and Sequel IIe system
- Sequencing, Analysis
Description
This document describes the procedure for installing SMRT Link v12.0. This document is for Customer IT or SMRT Link administrators. SMRT Link v12.0 supports RevioTM systems, Sequel® II systems and Sequel IIe systems. Sequel systems are not supported.
March 27, 2023
Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0
- Procedure & Checklist
- Revio system, Sequel II system and Sequel IIe system
- Library preparation
Description
This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.
March 21, 2023
SMRT Link Release Notes v12.0
- Release Notes
- Sequel II system and Sequel IIe system, Revio system
- Sequencing, Analysis
March 8, 2023
Operations guide — Revio system
- Guide
- Revio system
- Sequencing
Description
This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT® Link (v12.0) user guide.
March 8, 2023
SMRT Link web services API use cases v12.0
- Developer Documentation
- Sequel II system and Sequel IIe system, Revio system
- Sequencing, Analysis
Description
This document describes common tasks performed using the SMRT Link web services API and provides "how to" recipes for accomplishing these tasks.
March 8, 2023
SMRT Tools Reference Guide v12.0
- Developer Documentation
- Sequel II system and Sequel IIe system, Revio system
- Sequencing, Analysis
Description
This document describes the command-line tools included with SMRT® Link v12.0. These tools are for use by bioinformaticians working with secondary analysis results.
January 17, 2023
Overview — Nanobind HMW DNA extraction overview
- Guide & Overview
- Sequel II system and Sequel IIe system
- Sample preparation
Description
This overview provides links to all standard procedures for extracting HMW DNA by sample type and compatible Nanobind kit.
October 20, 2022
SMRT Link installation guide v11.1
- Guide & Overview
- Sequel II system and Sequel IIe system
- Analysis
Description
This document describes the procedure for installing SMRT Link v11.1 on a customer system. This document is for use by Customer IT.
October 20, 2022
SMRT Link release notes v11.1
- Release Notes
- Sequel II system and Sequel IIe system
- Analysis
Description
Release notes for SMRT Link v11.1.
October 8, 2022
Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0
- Procedure & Checklist
- Sequel II system, Sequel IIe system
- Library preparation
Description
This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
May 25, 2023
Guide & overview — Nanobind CBB kit
- Guide & Overview
- Sequel II system and Sequel IIe system
- Sample preparation
Description
This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.
May 25, 2023
Guide & overview — Nanobind plant nuclei kit
- Guide & Overview
- Sequel II system and Sequel IIe system
- Sample preparation
Description
This document describes procedures on how to use the Nanobind Plant Nuclei Big DNA Kit for extraction of HMW (50 kb – 300+ kb) DNA from plant nuclei.
May 25, 2023
Guide & overview — Nanobind tissue kit
- Guide & Overview
- Sequel II system and Sequel IIe system
- Sample preparation