January 16, 2025
Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0
- Procedure & checklist
- Sequel II system and Sequel IIe system, Vega system, Revio system
- Library preparation
Featured Documentation
This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation.
December 12, 2024
Operations guide — Revio system
- Guide
- Revio system
- Sequencing
Description
This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link user guide.
December 12, 2024
Release notes — Revio System instrument control software v13.3
- Release notes
- Revio system
- Sequencing, Analysis
Description
Release notes - Revio System instrument control software v13.3
April 10, 2025
Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0
- Procedure & checklist
- Revio system, Vega system, Sequel II system and Sequel IIe system
- Library preparation
Description
This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.
April 9, 2025
Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96
- Procedure & checklist
- Vega system, Revio system, Sequel II system and Sequel IIe system
- Library preparation
Description
This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.
March 27, 2025
Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing
- Procedure & checklist
- Revio system, Vega system
- Shearing, Library preparation, Sample preparation
Description
This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.
March 26, 2025
Operations Guide — Onso system cluster generator and short-read sequencer
- Guide
- Onso system
- Sequencing
Description
This guide describes the workflow for preparing Sequencing By Binding libraries for cluster generation on the PacBio Onso cluster generator system, as well as preparation for and sequencing of clustered flow cells on the Onso system.
March 24, 2025
Procedure & checklist — Preparing Onso libraries from genomic DNA for short read sequencing
- Procedure & checklist
- Onso system
- Library preparation
Description
This procedure describes the workflow for constructing Onso libraries from high molecular weight genomic DNA for sequencing on PacBio short-read sequencing systems. This procedure produces PCR-free and/or PCR- amplified libraries for clustering.
March 6, 2025
Procedure & checklist — Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0
- Procedure & checklist
- Vega system, Revio system, Sequel II system and Sequel IIe system
- Library preparation
Description
This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.
March 5, 2025
Release notes – Vega instrument control software v1.1
- Release notes
- Vega system
- Sequencing, Analysis
Description
Release notes for the Vega instrument control system software v1.1
March 5, 2025
SMRT Link Cloud Release Notes v25.2
- Release notes
- Vega system, Revio system
- Sequencing, Analysis
Description
SMRT Link Cloud release notes (v25.2)
March 5, 2025
SMRT Link Cloud User Guide v25.2
- Guide
- Vega system, Revio system
- Sequencing, Analysis
Description
SMRT Link Cloud user guide (v25.2)
March 5, 2025
SMRT Link Release Notes v25.2
- Release notes
- Vega system, Revio system
- Sequencing, Analysis
Description
SMRT Link release notes for v25.2
March 5, 2025
SMRT Link software installation guide v25.2
- Guide
- Vega system, Revio system
- Sequencing, Analysis
Description
SMRT Link software installation guide v25.2
March 5, 2025
SMRT Link v25.2 user guide
- Guide
- Revio system, Vega system
- Sequencing, Analysis