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Documentation

Welcome to the PacBio Documentation page. The table below allows you to search for and download the latest guides, protocols, product information, and more. For any questions about our documentation, please contact us.

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Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded Universal Primers for Multiplex SMRT Sequencing

Procedure & Checklist
Sequel II System, Sequel System
Template Preparation
April 29, 2020

Description +

The procedure describes a workflow for quickly constructing SMRTbell libraries for multiplexing PCR products using PacBio Barcoded Universal Primers and SMRTbell Express Template Prep Kit 2.0. This workflow uses a 2-step PCR for generating up to 96-barcoded amplicons with the first PCR step requiring user-supplied internal primers that contain a combination of universal and target-specific sequences. In the second PCR step, barcodes are incorporated by using universal sequences tailed with 16-bp PacBio barcode sequences. Once the PCR products are barcoded, they can be pooled into a single tube for SMRTbell library construction with the SMRTbell Express Template Prep Kit 2.0.

Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons

Procedure & Checklist
Sequel II System, Sequel System
Template Preparation
April 29, 2020

Description +

The procedure describes a workflow for quickly constructing SMRTbell libraries from PCR products using PacBio Barcoded Overhang Adapters and SMRTbell Express Template Prep Kit 2.0. In this workflow, a barcode is introduced to each amplicon through ligation with a hairpin adapter containing a 16-bp barcode. Once barcoded, amplicons can be pooled and purified for sequencing.

Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0

Procedure & Checklist
Sequel II System, Sequel System
Template Preparation
March 30, 2020

Description +

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.

SMRT Link Software Installation (v8.0)

Guide & Overview
Sequel II System, Sequel System
Analysis
January 28, 2020

Description +

This document describes the procedure for installing SMRT Link v8.0 on a customer system. This document is for use by Customer IT.

SMRT Link User Guide (v8.0)

Guide & Overview
Sequel II System, Sequel System
Analysis
January 28, 2020

Description +

This document describes how to use Pacific Biosciences’ SMRT Link software (v8.0). SMRT Link is the web-based end-to-end workflow manager for Sequel Systems.

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Increasing Solve Rates for Rare and Mendelian Diseases with Long-read Sequencing

 May 27, 2020

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