HiFiViral COVID-19 surveillance

Introducing HiFiViral for SARS-CoV-2 kit: a simple-to-use, scalable, cost-effective solution for sequencing the entire SARS-CoV-2 genome. This kitted solution provides robust performance on novel variants and comprehensive detection of all types of mutations.

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Benefits for pathogen surveillance

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Simple to use

The PacBio HiFiViral kit has less pipetting and fewer plate transfers than PCR-based enrichment assays. The add-only enrichment workflow further reduces handling errors with color-change indicators at every step.

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Robust performance

Our fully kitted solution uses a MIPs-based method that combines the simplicity and cost effectiveness of PCR with the variant-robustness of deeply tiled target capture methods. Each base is covered by 22 probes.

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Capture all variants

The combination of 675 bp inserts and the accuracy of HiFi sequencing enables comprehensive detection of all types of mutations and the ability to detect major and minor strains.

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Cost-effective

PacBio HiFi sequencing on the Sequel II systems is a cost-effective solution that provides built-in surge capacity to scale throughput on a single platform.

Spotlight

Illustrating the new workflow

This short video describes the simplicity of the new kitted solution, which will streamline your workflow and enable you to capture all variants, leading to the best quality results in your SARS-CoV-2 surveillance.

HiFiViral for SARS-CoV-2 workflow

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Go from RNA samples to analysis results in 28 to 42 hours, with 1 to 2.5 hours hands-on time.

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Multiplex 24–384 samples per SMRT Cell 8M, or load up to 8 SMRT Cells per Sequel IIe system to process up to 3,072 samples per week.

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Sequence and analyze overnight with one touch using SMRT Link v10.2 or later to produce submission-ready files, along with a performance summary and QC tools.

The SARS-CoV-2 enrichment workflow

HiFiViral kit workflow

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.