Benefits for pathogen surveillance
Simple to use
The PacBio HiFiViral kit has less pipetting and fewer plate transfers than PCR-based enrichment assays. The add-only enrichment workflow further reduces handling errors with color-change indicators at every step.
Our fully kitted solution uses a MIPs-based method that combines the simplicity and cost effectiveness of PCR with the variant-robustness of deeply tiled target capture methods. Each base is covered by 22 probes.
Capture all variants
The combination of 675 bp inserts and the accuracy of HiFi sequencing enables comprehensive detection of all types of mutations and the ability to detect major and minor strains.
PacBio HiFi sequencing on the Sequel II systems is a cost-effective solution that provides built-in surge capacity to scale throughput on a single platform.
Illustrating the new workflow
This short video describes the simplicity of the new kitted solution, which will streamline your workflow and enable you to capture all variants, leading to the best quality results in your SARS-CoV-2 surveillance.
HiFiViral for SARS-CoV-2 workflow
Go from RNA samples to analysis results in 28 to 42 hours, with 1 to 2.5 hours hands-on time.
Multiplex 24–384 samples per SMRT Cell 8M, or load up to 8 SMRT Cells per Sequel IIe system to process up to 3,072 samples per week.
Sequence and analyze overnight with one touch using SMRT Link v10.2 or later to produce submission-ready files, along with a performance summary and QC tools.