Library prep + barcoding

Reagent kits for preparing HiFi and Onso sequencing libraries

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HiFi library prep

SMRTbell prep kit 3.0PN: 102-182-700

This kit contains necessary reagents to prepare 24 SMRTbell® libraries. Cleanup beads are included. Barcoded adapters and size-selection reagents are sold separately.

AMPure PB beads size selection kitPN: 102-182-500

Paramagnetic beads and elution buffer to selectively deplete DNA fragments less than 5kb

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HiFi express template prep kit 2.0PN: 102-088-900

Reagent bundle for generating WGS HiFi libraries with the Express template prep kit 2.0

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HiFi barcoding

SMRTbell barcoded adapter plate 3.0PN: 102-009-200

96 SMRTbell barcoded overhang adapters in plate format (1 sample per barcoded adapter).

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Barcoded M13 primer platePN: 102-135-500

384 barcoded M13 primer pairs in plate format (1 sample per barcoded primer pair).

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Barcoded overhang adapter kit - 8A/8BPN: 101-628-400/500

Two sets of 8 barcoded overhang adapters for multiplexing SMRTbell libraries.

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Onso library prep

Available with the Onso system in 2023

Onso fragmentation DNA library prep kitPN: 102-499-100

Reagents to enzymatically fragment DNA and prepare libraries for sequencing on the Onso system.

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Onso DNA library prep kitPN: 102-431-400

Reagents prepare libraries for sequencing on the Onso system from fragmented DNA.

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Onso library amp kitPN: 102-410-800

Reagents to amplify libraries containing Onso indexed adapters.

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Onso library quant kitPN: 102-431-800

Reagents to quantify Onso-compatible libraries by qPCR.

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Onso library conversion kitPN: 102-529-500

Reagents to convert P5/P7 adapter libraries to be compatible with the Onso system.

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Onso indexed library control kitPN: 102-529-900

Spike-in control for sequencing runs to determine run quality and provide sequence diversity to the library pool.

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Onso blocking oligo kitPN: 102-431-600

Reagents for use in hybridization capture in place of P5/P7 blocking oligos.

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Onso barcoding

Available with the Onso system in 2023

Onso indexed adapter kitPN: 102-431-700

Adapters to generate indexed Onso libraries.

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