Library prep + barcoding

Long description of product.

Describe quantity of reagent.

Note: Sequel II and IIe Systems compatible.

• Resource: Procedure & Checklist — Preparing whole genome and metagenome libraries using SMRTbell® prep kit 3.0
• Product insert: SMRTbell prep kit 3.0
• Product insert: SMRTbell cleanup beads
• Product insert: Low TE buffer (pH 8.0)
• SDS: SMRTbell prep kit 3.0
• SDS: SMRTbell cleanup beads
• SDS: Low TE buffer (pH 8.0)

Long description of product.

Reagent quantity supplied at 5 mL volume.

• Resource: Procedure & Checklist — Preparing whole genome and metagenome libraries using SMRTbell® prep kit 3.0
• Product insert: AMPure PB beads
• Product insert: Elution buffer
• SDS: AMPure PB beads
• SDS: Elution buffer

The kit contains a bundle of SMRTbell express template prep kit 2.0 (100-938-900, 18 samples) and SMRTbell enzyme cleanup kit 2.0 (101-932-600, 18 samples). Includes sequencing primer v5 (102-067-400, 18 samples).

Describe quantity of reagent.

Note: Sequel II and Sequel IIe Systems compatible. Kit components are sold separately.

• Resource: Procedure & Checklist — Preparing HiFi SMRTbell Libraries Using SMRTbell Express Template Prep Kit 2.0
• Resource: Procedure & Checklist — Using AMPure PB Bead for Size Selection
• Product insert: SMRTbell express template prep kit 2.0
• Product insert: SMRTbell enzyme cleanup kit 2.0
• Product insert: Sequencing primer v5
• SDS: SMRTbell express template prep kit 2.0
• SDS: SMRTbell enzyme cleanup kit 2.0
• SDS: Sequencing primer v5

The Onso fragmentation library prep kit enables simultaneous fragmentation and end repair using a low-bias enzymatic fragmentation/end repair formulation to process genomic DNA samples. Fragmented/end repaired DNA is then ligated to Onso indexed adapters to generate libraries suitable for sequencing on the Onso system. Supports processing of any high-molecular gDNA at 10-1000ng as starting input.

Kit contains reagents to process up to 96 samples.

• Product insert: Onso fragmentation DNA library prep kit
• SDS: Onso fragmentation DNA library prep kit

The Onso library prep kit enables fragmented DNA inputs (e.g enzymatic, mechanically-sheared, or native) to undergo single-step end-repair and A-tailing reaction to prepare for ligation to Onso indexed adapters to generate libraries suitable for sequencing on the Onso system. The kit has been purpose-designed to be familiar to existing on-market library prep kits, allowing new and existing users to seamlessly incorporate into current workflows. Suitable sample types supported include cell-free DNA, PCR amplicons, and any pre-fragmented DNA at 10-1000ng input range.

Kit contains reagents to process up to 96 samples.

• Product insert: Onso DNA library prep kit
• SDS: Onso DNA library prep kit

The Onso library amp kit contains a 2X PCR master mix with primers designed to specifically amplify libraries containing Onso indexed adapters. The resulting amplicons can be used for library QC , as input to downstream applications (e.g. target enrichment), and as input to the Onso clustering/sequencing process.

The kit contains Onso-specific primers and 2X PCR master mix, good for 96 reactions.

• Product insert: Onso library amp kit
• SDS: Onso library amp kit

The Onso library quant kit enables accurate quantification of Onso-compatible libraries by qPCR. The quant kit contains six standards ranging from 100pM to 0.001pMContains qPCR master mix, Onso-specific PCR primers, dilution buffer, and ROX dyes (hi/lo) for use across different qPCR instruments.

Kit contains reagents for 500 reactions.

• Product insert: Onso library quant kit
• SDS: Onso library quant kit

The Onso library conversion kit enables standard P5/P7 adapter libraries to be easily converted to libraries compatible with the Onso system. PCR conditions have been optimized to maintain library fidelity during the conversion process.

The kit contains Onso-specific primers and 2X PCR master mix, good for 96 reactions.

• Product insert: Onso library conversion kit
• SDS: Onso library conversion kit

The Onso indexed library control is a lambda phage-based control used as a spike-in control for sequencing runs to determine run quality as provide sequence diversity to the library pool. Provided as an indexed library at 2nM concentration, ready to dilute and spike-in to library pools prior to clustering and sequencing.

The kit is provided as an indexed library at 2nM concentration, ready to dilute and spike-in to samples of interest prior to clustering and sequencing.

• Product insert: Onso indexed library control
• SDS: Onso indexed library control

The Onso blocking oligo kit is designed to be utilized in hybridization capture-based workflows in place of traditional P5/P7 blocking oligos. With optimized design specific to Onso indexed adapters, it decreases the frequency of non-specific binding of adapter sequences during the hybridization reaction.

Kit contains reagents for 96 reactions.

• Product insert: Onso blocker oligos
• SDS: Onso blocker oligos