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Library prep + barcoding

Reagent kits for preparing HiFi and Onso sequencing libraries

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SMRTbell library prep for HiFi sequencing

SMRTbell cleanup bead kitPN: 103-306-300

The SMRTbell cleanup bead kit contains reagents for DNA dilution and post-shearing cleanup using automated liquid handler systems. The kit includes SMRTbell cleanup beads and low TE buffer.

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SMRTbell prep kit 3.0PN: 102-182-700

The SMRTbell prep kit 3.0 contains the necessary reagents for library preparation with SMRTbell adapters. The kit also includes SMRTbell cleanup beads and low TE buffer.

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AMPure PB beads size selection kitPN: 102-182-500

The AMPure PB beads size selection kit contains paramagnetic beads and elution buffer to selectively purify double-stranded DNA less than 5 kb.

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HiFi express template prep kit 2.0PN: 102-088-900

The HiFi express template prep kit 2.0 contains reagents for generating WGS HiFi libraries with the SMRTbell express template prep kit 2.0. It includes the SMRTbell enzyme cleanup kit and Sequencing primer v5.

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HiFi barcoding

Barcoded overhang adapter kit 8BPN: 101-628-500

The barcoded overhang adapter kit 8B contains 8 SMRTbell barcoded overhang adapters (6 reactions per barcoded adapter, for a total of 48 reactions).

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SMRTbell adapter index plate 96APN: 102-009-200

The SMRTbell adapter index plate 96A contains 96 indexed SMRTbell adapters in plate format (1 sample per index). The plate includes indexes bc2001–bc2096.

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Barcoded M13 primer platePN: 102-135-500

The barcoded M13 primer plate contains 384 barcoded M13 primer pairs in plate format (1 sample per barcoded primer pair). It includes forward barcodes bc1002–bc1017 and reverse barcodes bc1050–bc1073.

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Barcoded overhang adapter kit 8APN: 101-628-400

The barcoded overhang adapter kit 8A contains 8 SMRTbell barcoded overhang adapters (6 reactions per barcoded adapter, for a total of 48 reactions).

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Onso library prep

Onso fragmentation DNA library prep kitPN: 102-499-100

The Onso fragmentation DNA library prep kit is for the creation of Onso-compatible libraries from unfragmented, HMW DNA. The kit includes reagents for fragmentation, end-repair, A-tailing, and adapter ligation of DNA fragments.

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Onso DNA library prep kitPN: 102-431-400

The Onso DNA library prep kit is for the creation of Onso-compatible libraries from fragmented DNA. The kit includes reagents for end-repair, A-tailing, and adapter ligation of DNA fragments.

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Onso library amp kitPN: 102-410-800

The Onso library amp kit contains reagents for the amplification of A/P1-tailed libraries for clustering on the Onso cluster generator. The kit includes Onso-specific A/P1 primers and 2× PCR master mix.

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Onso library quant kitPN: 102-431-800

The Onso library quant kit contains reagents for quantification of A/P1-tailed libraries. It also includes qPCR reagents, standards, dilution buffer, ROX (hi/lo), and universal PCR primers.

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Onso library conversion kitPN: 102-529-500

The Onso library conversion kit contains reagents for the conversion of libraries containing P5/P7 adapter sequences to Onso-compatible libraries.

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Onso indexed library control kitPN: 102-529-900

The Onso indexed library control kit contains ready-to-use lambda spike-in control for Onso sequencing runs to determine run quality and provide sequence diversity to the library pool.

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Onso blocker oligosPN: 102-431-600

The Onso blocker oligos enable increased specificity for Onso-indexed adapter libraries in hybrid capture applications.

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Onso barcoding

Available with the Onso system in 2023

Onso indexed adapter platePN: 102-431-700

The Onso indexed adapter plate contains indexed adapters suitable for combinatorial dual indexing of DNA libraries. The plate includes 96 different pairs of indices in plate format.

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