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Explore PacBio’s educational videos

PacBio  |    |  2022

Onso system by PacBio – a new standard in short-read sequencing

The Onso system is an innovative benchtop short-read DNA sequencing platform with an unprecedented level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.
PacBio  |  PacBio  |  2022

Revio and Onso – long-read and short-read sequencing with unmatched accuracy

The Revio long-read sequencer introduces long reads at a lower cost and higher throughput. The Onso short-read sequencer delivers short reads with unmatched accuracy. Combined, the completeness of long reads with the accuracy of short reads provides the most comprehensive view of genomes, transcriptomes, and epigenomes.
Hanscom, Terrence and Selby, Donald and Tseng, Elizabeth  |  PacBio  |  2022

Highly Accurate HiFi Reads for Discovery, Design, and Manufacturing of AAV Gene Therapy

Gene therapy is at an inflection point and experiencing tremendous growth. High accuracy and complete visibility are critical to the success of novel vector discovery, vector design, and manufacturing quality control for gene therapy products. This webinar illustrates why PacBio HiFi sequencing is the ideal solution due to its ability to sequence full length AAV genomes at high accuracy, which allows for detection of fragmentation, mutations, and large structural events. Short reads, qPCR, and less accurate long-read technology can miss important changes that may be present in the AAV sequence, potentially affecting the safety and efficacy of gene therapy products.
Feng, Xiaowen and Portik, Daniel and Wilkinson, Jeremy  |  Dana-Farber Cancer Institute, Harvard Medical School; PacBio  |  2022

HiFi metagenomics: more samples, more species, more MAGs

Learn how to cost-effectively level up your metagenomics research from expert bioinformaticians. PacBio HiFi sequencing is setting a new standard for quality and resolution in metagenome sequencing thanks to significant strides in data analysis tools. New pipelines that leverage HiFi sequencings unique combination of high accuracy and long read length yield low abundance species at a higher rate, with higher resolution and precision, and return more circular, single contig metagenome-assembled genomes (MAGs).
Liu, Christine and Turner, Tychele  |  University of California, San Diego, and Washington University School of Medicine  |  2022

Unlocking the Genome with Long-Read Sequencing in Genetic Disease Research

Follow two scientists on their journey of discovery using whole genome sequencing and single-cell RNA sequencing to shed light on previously unresolved medical mysteries. In this video, they share how they have been able to successfully identify the genetic drivers in disease research by accessing the complete genomic landscape, including regions previously deemed impossible to sequence.
  |  PacBio  |  2022

Introducing a whole new PacBio

To pioneer the future through biology, you have to look beyond the status quo and see the whole picture. At PacBio, we are devoted to empowering your vision, by delivering trustworthy technologies and the highest accuracy data. Our mission is to enable the promise of genomics to better human health. We create the world’s most advanced sequencing systems that provide the insights scientists need to improve our understanding of biology. We proudly support and collaborate with the scientific community as we work together to reveal the full potential of the genome. We strive to miss nothing, so you can do anything.
Tseng, Elizabeth  |  PacBio  |  2021

Allele-Specific, Isoform-Resolution Single-Cell RNA-Seq Analysis Using Long-Read Sequencing on Concatenated Single-Cell Molecules

In this talk, Dr. Elizabeth Tseng demonstrates a throughput increase for the scIso-Seq method by concatenating single-cell molecules, increasing yield a minimum of 6-fold per SMRT Cell 8M. She explains the bioinformatics workflow for analyzing concatenated scIso-Seq data, which begins with de-concatenation, followed by tagging of UMI and barcode information that can be processed by the isoseq3 pipeline for deduplication. Reads are then aligned against the reference genome, followed by SQANTI3 for transcript classification against a reference annotation (ex: GENCODE) which produces an isoform-level sparse matrix to be analyzed with single-cell tools such as Seurat. She also shows how to apply isoform-level phasing using a tool called IsoPhase, by first calling SNPs based on the deduplicated aligned reads, then using the full-length read information to identify the haplotypes, and finally assigning each isoform-haplotype its associated read counts. Since each read can be associated with its distinct cell type, this allows quantifying isoform expressions in an allele-specific and cell type-specific manner. Finally, she shows how this can be visualized using both standard genome browsers like IGV and long read-aware tools such as SWAN.
Kingan, Sarah  |  PacBio  |  2021

HiFiViral: A Novel Method for Surveillance of SARS-CoV-2 that is Robust Across Sample Input Quantities and the Evolution of New Variants

The COVID-19 pandemic continues to be a major global epidemiological challenge with the ongoing emergence of new strain lineages that are more contagious, more virulent, drug-resistant, and in some cases evade vaccine-induced immunity. In response, the HiFiViral SARS-CoV-2 kit was developed as a scalable solution for the Sequel II and Sequel IIe Systems. Unlike amplicon sequencing, the HiFiViral SARS-CoV-2 kit uses tiled probes, resulting in robust genome coverage across varying viral input quantities despite the presence of new variants. The use of highly accurate long reads, or HiFi reads, enables comprehensive variant detection, including single nucleotide variants, indels, and structural variants, as well as phasing of variants if multiple strains are present in samples. The fully kitted solution contains all reagents needed for viral enrichment and barcoding up to 384 samples, which can be pooled in a single SMRTbell library and run on a single SMRT Cell 8M. The add-only workflow requires two days in the lab with overnight sequencing and analysis and may be automated to achieve a turnaround time of 24 hours. SMRT Link analysis includes variant calling, HiFi read depth plots, the detection of multiple strains in samples, and consensus sequences ready for submission to public databases. In the talk, Dr. Sarah Kingan, demonstrates performance across a broad range of sample Ct, the accuracy of our variant calling method for viral controls, including variants of concern, and the ability to detect multiple variants down to 20% minor frequency. HiFiViral for SARS-CoV-2 is a cost-effective, convenient, and accurate method for viral sequencing, well-suited for scalable surveillance of a rapidly evolving virus to inform public health decision making.
Wenger, Aaron  |  PacBio  |  2021

Methylation Detection with PacBio HiFi Sequencing

In this talk, Dr. Aaron Wenger, describes how PacBio HiFi reads (15 kb - 25 kb, >99.9% accuracy) provide the most complete view of human genetic variation, including small variants in difficult-to-map regions and structural variants genome-wide. Further, PacBio sequencing simultaneously detects epigenetic modifications without requiring a specialized library preparation step like bisulfite conversion. This ability is commonly used to characterize epigenetic marks in bacterial genomes. Recent improvements in read length and data analysis have extended the ability to include the 5mC methylation that is present at CpG sites in human genomes. Using a deep learning model that integrates sequencing kinetics and base context, the accuracy of 5mC detection in humans for individual HiFi reads is around 80%. Combining multiple reads, the concordance to EMseq and bisulfite sequencing reaches around 90%. The single-molecule resolution of methylation, together with phasing from accurate long reads, allows the detection of allele-specific methylation patterns such as parental imprinting. This ability to detect bases and modifications allows HiFi sequencing to provide the most complete genome and epigenomes with a single technology and library preparation.
Gonzaludo, Nina  |  PacBio  |  2021

Using HiFi Reads for Improved and Accurate Haplotyping and Phasing of Pharmacogenomic Alleles

Pharmacogenomics (PGx) utilizes genomic information to assess an individual’s response to certain medications and can be used to predict adverse drug reactions or decreased efficacy. While numerous assays and genetic tests have been developed to interrogate pharmacogenes, several limitations exist, including lack of phasing information, and poor detection in complex regions with structural variants, pseudogenes, or gene conversions. In this talk, Dr. Nina Gonzaludo, describes amplicon and targeted enrichment capture SMRT Sequencing workflows that generate HiFi reads for high resolution of PGx alleles. To fully resolve CYP2D6, a highly polymorphic gene in a region with extensive homology, she discusses an amplicon strategy that resulted in ≥99.9% accuracy per sequencing run and >99% demultiplexed on target reads to CYP2D6. Additionally, results show improved haplotyping accuracy over orthogonal technologies in a set of 22 reference samples. Separately, she discusses preliminary results from a panel enrichment strategy for high resolution genotyping and phasing of 43 clinically significant pharmacogenes. These approaches can be leveraged as cost-effective and highly accurate methods for advancing PGx research and discovery.
Henry, Christian and Shen, Richard and Stone, Jennifer  |  PacBio  |  2021

With Long Reads and Short Reads, the Possibilities are Endless – PacBio ASHG 2021 Fireside Chat

With the September 2021 closing of PacBio’s acquisition of Omniome, PacBio intends to become the first company to offer both long-read and short-read sequencing platforms. What does this mean for customers? How is PacBio leadership thinking about delivering a differentiated set of products and applications into high-growth clinical markets? In this intimate conversation with genomics leaders, Christian Henry, and Richard Shen, they share their vision for the future as a combined company.
Stone, Jennifer  |  PacBio  |  2021

HiFi Sequencing: See What You’ve Been Missing

PacBio Vice President of Segment Marketing, Dr. Jennifer Stone, demonstrates how HiFi sequencing is changing the game in human genetics by sharing some of the exciting milestones and seminal publications our technology has produced this year.
Farrow, Emily  |  Children’s Mercy Kansas City  |  2021

Integrated Rare Disease using Long-Read Genome Sequencing

Genomic variation beyond single nucleotide variants, including structural variation (SV), copy number variants (CNV), and repeat expansions, plays a significant role in rare disease. However, current technologies require multiple tests to fully access these variants, resulting in complex testing algorithms and the potential for missed diagnoses. Long-read genome sequencing offers the ability to accurately detect SNV, CNV, SV, and expansions with a single test. This presentation will highlight the integrated analyses offered by HiFi sequencing, using case examples demonstrating the potential for a unified test.
Al’Khafaji, Aziz  |  Broad Institute  |  2021

Scalable RNA Isoform Sequencing using Intramolecular Multiplexed cDNAs

While RNA-sequencing has dramatically accelerated our understanding of biology, quantitation and discovery of full-length RNA isoforms resulting from alternative splicing remain poorly resolved. Alternative splicing is a core regulatory process that modulates the structure, expression, and localization of expressed proteins through differential exon and/or UTR splicing during transcript maturation. Beyond being an integral component of cellular development and homeostatic maintenance, RNA splicing is implicated in a wide range of pathologies with hallmark isoforms being linked to cardiovascular, neurological, and immunological diseases. Current limitations in isoform quantitation and discovery arise from the inability of existing sequencing platforms to scalably sequence full-length mRNAs – short-read approaches are unable to span most successive splice sites while long-read approaches are constrained by limited throughput. To enable scalable full-length RNA sequencing, we have developed the method Multiplexed Arrays sequencing (MAS-seq) which maximizes the sequencing potential of isoforms on the PacBio platform. Through the use of deoxy-uracil digestion followed by barcode-directed ligation of cDNAs, MAS-seq generates long multiplexed cDNA arrays with a length distribution that allows for both accurate consensus sequencing and optimal capacity utilization of PacBio sequencers. In combination with upstream artifact depletion measures, MAS-seq boosts the sequencing throughput to approximately 40 million full-length transcripts per SMRT Cell 8M, a >20-fold increase compared to PacBio’s scIso-Seq workflow. We sequenced synthetic RNA isoform standards (Lexogen) and demonstrate a 99.8% isoform identification accuracy using MAS-seq, far exceeding the 56.8% accuracy of Smart-seq3, the best-in-class short-read isoform identification protocol. MAS-seq-enabled single-cell RNA isoform sequencing of tumor infiltrating CD8+ T cells robustly identifies the canonical CD45 (PTPRC) isoforms associated with the range of observed T cell states - a finding additionally validated at the protein level via CITE-seq. Further, we demonstrate the impact of the long-read sequencing throughput gains on single-cell isoform analysis enabled by MAS-seq, providing a 44% increase in single-cell clustering capacity (adjusted Rand index) and a 34-fold gain in identifying differentially spliced genes amongst the CD8+ T cell subtypes. MAS-seq is a streamlined and cost-effective approach that enables scalable bulk and single-cell RNA isoform sequencing.
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