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Explore PacBio’s educational videos

PacBio  |    |  2022

Onso system by PacBio – a new standard in short-read sequencing

The Onso system is an innovative benchtop short-read DNA sequencing platform with an unprecedented level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.
PacBio  |  PacBio  |  2024

The value of Q40+ sequencing accuracy with SBB for liquid biopsy

Liquid biopsy is revolutionizing cancer research through non-invasive detection of tumor DNA in the blood. However, sequencing errors introduced by traditional next-generation sequencing can mimic real mutations, obscuring the true signal. The PacBio Onso short-read sequencing system, powered by our highly accurate SBB technology, can reduce false positives and improve sensitivity for detecting rare variants in ctDNA, potentially enabling a new generation of liquid biopsy assays for more sensitive tumor DNA detection.
PacBio  |  PacBio  |  2024

Transcript detection and quantification using Kinnex full-length RNA sequencing data

In this webinar, learn how David Wissel from ETH Zurich uses MAS-Seq technology, integral to Kinnex kits, to enhance throughput for lrRNA-Seq, facilitating more comprehensive transcript quantification. The analysis contrasts Kinnex data with short-read RNA-Seq using spike-in RNA variant control mixes and assesses its efficacy in differential transcript expression and usage. Additionally, the webinar introduces an accessible Snakemake workflow, tailoring bioinformatics processes for efficient isoform analysis in multi-sample studies. Uncover the bioinformatics frontier in RNA sequencing research!
PacBio  |  PacBio  |  2024

Bioinformatics resources to analyze PacBio HiFi human genomes

Discover how HiFi human genome sequencing, paired with cutting-edge bioinformatics, is reshaping our understanding of human biology and diseases. This webinar delves into a comprehensive bioinformatics pipeline, addressing a vast range of variant types. From standard single-nucleotide variants and indels to the more complex structural variants and tandem repeats - HiFi sequencing, in conjunction with sophisticated bioinformatics, provides profound insights and clarity.
PacBio  |  PacBio  |  2024

A more complete view of cancer—get genome, epigenome, & transcriptome with HiFi long-read sequencing

Do you have a comprehensive view of the cancer genome, epigenome and transcriptome in your research? Learn how highly accurate HiFi long-read sequencing can provide you with accurate detection of small variants and the ability to phase them. With the throughput and scale of Revio you can now confidently detect somatic variants in tumor samples without leaving anything behind.
  |  PacBio  |  2024

Increasing the throughput of full-length 16S and RNA-Seq with Kinnex kits

Explore the transformative capabilities of Kinnex kits from PacBio, in reshaping the landscape of microbiome community profiling and RNA-Seq in plant and animal research. This webinar guides you through the enhanced functionalities of full-length 16S rRNA and RNA-Seq, empowered by the new Kinnex kits (using MAS-Seq technology/method), which facilitate a substantial increase in the number of reads obtained from PacBio long-read sequencing platforms. Delve into detailed customer scientific studies that showcase the practical applications and influential impact of Kinnex kits in contemporary research environments.
PacBio  |  PacBio  |  2023

Advancing Cardiology Research with Long-read RNA Sequencing

In this webinar, learn how long-read transcriptomics at full-length isoform resolution is transforming our understanding of heart development, function, and disease. Two researchers discuss their use of bulk and single-cell sequencing with the PacBio Iso-Seq method for cardiology research, which includes a comparison to Oxford Nanopore and short-read RNA sequencing, discuss applications in spatial transcriptomics, and review early data generated from the recently released Kinnex full-length RNA kit.
PacBio  |  PacBio  |  2023

Expanding NGS truth sets with long-reads: more comprehensive variant detection in a multi-generational pedigree

Accurate long-read sequencing characterizes the full spectrum of genetic variation across the genome, but variant calling software is still catching up to the sequencing technologies. We have generated deep Pacific Biosciences (PacBio) high-fidelity (HiFi), ultra-long Oxford Nanopore Technologies (ONT), Strand-seq, and Illumina whole-genome sequencing data to construct near-T2T, phased genome assemblies from primary material obtained from a 4-generation, 28-member CEPH pedigree (1463). We are constructing a more comprehensive and validated catalog of greater than 8 million single-nucleotide variants, indels, short tandem repeats, and structural variants, including a detailed assessment of inversion polymorphisms that associate with disease risk. The use of multiple orthogonal technologies, near-T2T phased-genome assemblies, and a multi-generation family allow us to assess inheritance patterns and to create a “truth set” for all classes of human genetic variation upon which to test and benchmark new technologies.
PacBio  |  PacBio  |  2023

Exploring the potential clinical utility of HiFi sequencing for homologous loci (pseudogenes)

PacBio HiFi long-read sequencing has the potential to become a single front-line assay for interrogating rare disease cohorts because of its ability to accurately call and phase all classes of variants. An international group of clinical researchers and PacBio have been collaborating to study the efficacy of HiFi sequencing in interrogating the many possible genetic mechanisms that underlie rare diseases. This presentation provides an overview of the group’s goals and activities and recent results, particularly around correctly resolving clinically relevant genes that lie in areas of high sequence homology or otherwise complex regions of the human genome.
PacBio  |  PacBio  |  2023

Achieving more comprehensive variant detection at scale in rare disease research

Higher throughput and lower costs for highly accurate long-read (HiFi) sequencing are now available on the Revio system. HiFi sequencing enables you to capture all variant types with high precision and recall, phase haplotypes, and identify methylation patterns across the genome. Dr. Adam Ameur of SciLifeLab in Uppsala, Sweden, has implemented the Revio system to create new discovery opportunities for a range of HiFi sequencing applications, including whole-genome sequencing in human genomic research.
PacBio  |  PacBio  |  2023

Understanding SMRT Link run QC and on-instrument analysis on the PacBio Revio system

In this webinar, PacBio scientists Nancy Francoeur, PhD and Caroline Storer, PhD will help you understand Revio performance metrics and on-instrument analysis options to put you in control of your HiFi sequencing runs. Primary run QC metrics in SMRT Link let you evaluate sequencing performance, monitor performance trends, and understand how sample loading affects HiFi sequencing quality and yield. Watch this webinar to find out more about: HiFi sequencing primary run QC metrics and terminology. Interpretation of key metrics and visualizations to evaluate sequencing performance. Revio system on-instrument analysis pipelines, output files, and folder structure.
PacBio  |  PacBio  |  2023

Library preparation and HiFi sequencing applications for AAV

Gain a deep understanding of the unique advantages that HiFi sequencing brings to the table, empowering you to make informed decisions that can significantly impact the safety and efficacy of gene therapy products. In this exciting webinar, Edward Oakeley, PhD, Novartis, shares and explains his experience and the developments of the HiFi AAV genome sequencing method over time and how his group has developed a modified library preparation protocol to maximize the usefulness of PacBio HiFi reads for AAV characterization. The modified library prep protocol includes adding an additional step for the synthesis of a complementary second strand in order to capture a greater variety of AAV molecules in the analysis.
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