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Nanobind + SRE Sample Prep kits

Innovative solutions for high-molecular-weight (HMW) DNA sample prep

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Nanobind DNA extraction kits

Extract HMW DNA from a wide variety of sample types.

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NANOBIND MAGNETIC DISKS

  • Nanostructured surface binds DNA and protects it from shearing
  • Extract high molecular weight (50–300+ kb)
  • Produce high extraction yields with high purity

RAPID BIND, WASH, AND ELUTE

  • Process most sample types in only 1–4 hours
  • Uses a single disk per tube
  • Supports manual or automated processing
  • No proprietary equipment required for manual extraction

DIVERSE SAMPLE TYPES ALL VALIDATED BY SEQUENCING

  • Cultured cells and bacteria
  • Blood
  • Plants
  • Tissues
  • Developmental sample types include insects and fungal

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Brochure

Automate DNA extraction and optimize your sample prep workflow for HiFi sequencing

PacBio® Nanobind® HT kits enable automation of DNA extraction, the first step of an end-to-end workflow that harnesses the power of HiFi sequencing. Nanobind HT kits are designed for whole human blood and mammalian cell samples and are compatible with four robotic platforms.

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Nanobind + SRE sample prep kits

Remove short DNA from your sequencing samples

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SRE SIZE SELECTION

  • Deplete fragments from extracted DNA prior to library preparation
  • Fast, easy, and inexpensive
  • High recovery

SIMPLE SIZE SELECTIVE PRECIPITATION

  • HMW DNA precipitated by centrifugation
  • Low molecular weight (LMW) DNA is washed away
  • Process many samples in parallel in 1 hour
  • No proprietary equipment required for manual extraction

VARIABLE DEPLETION CUTOFF

  • Progressive depletion begins at cutoff with depletion efficiency increasing as DNA gets shorter
  • SRE XS depletes DNA <10 kb
  • SRE depletes DNA <25 kb
  • SRE XL depletes DNA <40 kb

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Procedure & checklist

Removing short DNA fragments with the Short Read Eliminator (SRE) kit

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation. The SRE kit is used for rapid size selection of unsheared HMW DNA samples. This method can significantly enhance mean read length by progressively depleting short DNA up to 10 kb.

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NANOBIND KITS IN ACTION

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Procedure + Checklists

Quick and easy reference guides to learn all about Nanobind kits and how they enable HMW DNA extraction.

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Guides + Overviews

Nanobind guides provide recommendations for hard-to-sequence sample types.

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Product inserts + SDS

Download product inserts and SDS.

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EXPLORE

Did you know we have other resources for reports, papers, and videos?

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END-TO-END WORKFLOWS FOR LONG-READ SEQUENCING AT SCALE

Extract the highest quality high molecular weight (HMW) DNA using Nanobind DNA extraction kits.

Enhance read lengths by depleting short DNA using SRE size selection kits.

Our experts offer end-to-end support from extraction through library prep and sequencing.

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If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.