Nanobind + SRE Sample Prep kits
Innovative solutions for high-molecular-weight (HMW) DNA sample prep
NANOBIND MAGNETIC DISKS
- Nanostructured surface binds DNA and protects it from shearing
- Extract high molecular weight (50–300+ kb)
- Produce high extraction yields with high purity
RAPID BIND, WASH, AND ELUTE
- Process most sample types in only 1–4 hours
- Uses a single disk per tube
- Supports manual or automated processing
- No proprietary equipment required for manual extraction
DIVERSE SAMPLE TYPES ALL VALIDATED BY SEQUENCING
- Cultured cells and bacteria
- Blood
- Plants
- Tissues
- Developmental sample types include insects and fungal
Brochure
Automate DNA extraction and optimize your sample prep workflow for HiFi sequencing
PacBio® Nanobind® HT kits enable automation of DNA extraction, the first step of an end-to-end workflow that harnesses the power of HiFi sequencing. Nanobind HT kits are designed for whole human blood and mammalian cell samples and are compatible with four robotic platforms.
SRE SIZE SELECTION
- Deplete fragments from extracted DNA prior to library preparation
- Fast, easy, and inexpensive
- High recovery
SIMPLE SIZE SELECTIVE PRECIPITATION
- HMW DNA precipitated by centrifugation
- Low molecular weight (LMW) DNA is washed away
- Process many samples in parallel in 1 hour
- No proprietary equipment required for manual extraction
VARIABLE DEPLETION CUTOFF
- Progressive depletion begins at cutoff with depletion efficiency increasing as DNA gets shorter
- SRE XS depletes DNA <10 kb
- SRE depletes DNA <25 kb
- SRE XL depletes DNA <40 kb
Procedure & checklist
Removing short DNA fragments with the Short Read Eliminator (SRE) kit
This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation. The SRE kit is used for rapid size selection of unsheared HMW DNA samples. This method can significantly enhance mean read length by progressively depleting short DNA up to 10 kb.
NANOBIND KITS IN ACTION
NANOBIND
PUBLICATIONS
Read how scientists are using their projects. Sex chromosome transformation and the origin of a male-specific X chromosome in the creeping vole
Procedure + Checklists
Quick and easy reference guides to learn all about Nanobind kits and how they enable HMW DNA extraction.
Guides + Overviews
Nanobind guides provide recommendations for hard-to-sequence sample types.
END-TO-END WORKFLOWS FOR LONG-READ SEQUENCING AT SCALE
Extract the highest quality high molecular weight (HMW) DNA using Nanobind DNA extraction kits.
Enhance read lengths by depleting short DNA using SRE size selection kits.
Our experts offer end-to-end support from extraction through library prep and sequencing.
