Short-read sequencing:
Sequencing by binding

Breakthrough next generation sequencing technology to begin a new era in short-read sequencing

Request quote

Tertiary hero overlay

Introduction to sequencing by binding

Sequencing by binding (SBB) chemistry delivers a breakthrough in sequencing accuracy. Compared to sequencing by synthesis (SBS) utilized by traditional short-read sequencers, SBB chemistry gives ~15x lower error rates at any given cycle so that you can achieve near-perfect accuracy in short-read sequencing without having to sequence at unnecessary depths.

Sprite decoration
Roundel image of user inserting tray into Onso system sequencer - PacBio

Sequencing by binding on the Onso system

The Onso system is an integrated hardware and software short-read sequencing platform focused on delivering best-in-class accuracy, resulting in over 90 issued patents and numerous pending applications in the US and abroad. The Onso system utilizes SBB chemistry to free researchers from the limitations of existing short-read sequencing platforms, providing ≥90% of bases at Q40+ accuracy.

Onso specifications   Onso brochure


Sequencing 101: SBB sequencing

Since the inception of next generation sequencing (NGS) more than a decade ago, short-read sequencing accuracy has seen only marginal improvement. Having achieved a level of precision thought to be…

Learn more

Sprite decoration


Onso system’s short-read sequencing chemistry

A major contributor to the Onso system’s highly accurate reads is the chemistry. Proprietary sequencing by binding (SBB) chemistry uses native nucleotides, scarless incorporation, and optimized conditions for binding and extension. These innovations result in data with decreased errors relative to competing platforms and, as a result, allow detection of rare variants below the limit of detection of existing platforms as well as resolving regions in the genome. We believe SBB will provide new opportunities for advancement of biology through highly accurate next generation sequencing.

Product note


SBB is an innovative sequencing technology that delivers amazing NGS accuracy. By separating the binding and incorporation steps, optimizing the chemistries for each step, and using native nucleotides, greater signal to noise can be achieved resulting in higher accuracy base calls.

Learn more

Sequencing by binding solutions

black cancer research icon

Cancer research

Enable development of screening, monitoring, and therapy selection

black gene-editing icon

Gene editing research

Confirmation of potential editing outcomes at extraordinary sensitivity

black exome panels icon


Validated whole exome sequencing (WES) using third-party hybrid capture protocols

black single-cell icon


3’ single-cell RNA-Seq libraries supported and validated on the Onso system


Why SBB could be a game-changer for liquid biopsy research

In this article we will investigate how an important new PacBio short-read sequencing chemistry called sequencing by binding (SBB) has the potential to help revolutionize liquid biopsy and cancer diagnostic research.

Learn more

Sprite decoration


The Onso system is an innovative benchtop short-read sequencing platform that uses PacBio SBB technology to produce an unprecedented level of accuracy.

Learn more

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.