Human transcriptome studies
Explore how changes in isoform usages contribute to the phenotypic differences between health and disease.
Plant + animal transcriptome studies
Advance breeding programs and basic research with full-length cDNA sequencing that does not require a reference genome.
Introduction to RNA sequencing using the Iso-Seq method
With long and accurate HiFi reads, you can characterize the full diversity of the transcriptome:
- See alternative start and end sites
- Characterize splicing events
- Identify allele-specific isoforms
- Profile expression at isoform resolution
- Detect fusion genes
- Predict full-length open read frames
- …and more
“More than 50% of isoforms robustly detected using Iso-Seq could not be readily recapitulated using standard RNA-seq, highlighting the advantage of long-read sequencing for characterizing isoform diversity.”1
– Leung, et al, (2021)
1. Leung, et al. (2021) Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing. Cell Rep. 37(7):110022.
RNA sequencing with Iso-Seq analysis in action
New isoform phasing technique traces parental-progeny differences in maize
See how Cold Spring Harbor scientists endeavored to ensure that corn progeny could outperform their ‘parents.’
Scalable RNA isoform sequencing using intra-molecular multiplexed cDNAs
For scalable full-length RNA sequencing, we have developed multiplexed arrays sequencing…
Iso-Seq analysis enthusiasts share research wins at virtual social event
Dozens of self-proclaimed Iso-Seq analysis geeks and other curious researchers gathered…
Isoform-level transcriptome reference helps shrimp ward off disease
Shrimp are one of the fastest growing global foods, but also one of the most vulnerable.
SHAPING THE FUTURE
Washington State University researchers use Iso-Seq analysis to shed light on the metabolic mystery of brown bears.
Iso-Seq application workflows at a glance
Advantages of RNA sequencing with the
Powered by HiFi reads, the Iso-Seq method uncovers biology that other approaches miss.
Iso-Seq Method delivers full-length transcript isoforms, no assembly required
Unlike short-read RNA-seq methods, Iso-Seq method reads span the whole transcript, capturing even untranslated regions.
- Detect alternative polyadenylation
- Detect differential isoform usages (DIU)
- Obtain isoform-level phasing information
Iso-Seq Data is comprehensive
Characterize whole transcriptome isoform diversity that can reveal complex alternative splicing, fusion events, and transcriptional readthroughs.
Iso-Seq Analysis offers unparalleled insight at the single cell level
Use any single-cell platform to generate full-length cDNA as input for SMRT sequencing and achieve all the same benefits of Iso-Seq on bulk cDNA — complete transcript sequence, isoform-level phasing, and isoform profiling — at single-cell resolution.
TECHNOLOGY DEVELOPMENT SPOTLIGHT
MAS-ISO-Seq method delivers >15× throughput through concatenation
Our technology is constantly evolving. MAS-ISO-Seq data takes advantage of long and accurate HiFi read lengths to increase Iso-Seq throughput by >15-fold through multiplexed arrays of cDNA molecules.