The future of RNA sequencing is with long reads! The Iso-Seq method sequences the entire cDNA molecules – up to 10 kb or more – without the need for bioinformatics transcript assembly, so you can characterize novel genes and isoforms in bulk and single-cell transcriptomes and further:
- Characterize alternative splicing (AS) events, including alternative start sites, end sites, intron retention, and exon-skipping events
- Find gene fusions in tumor samples
- Identify allele-specific isoforms
- Detect differentially expressed isoforms and isoform switching events
- Predict functional impact of novel isoforms through open reading frame (ORF) prediction
See how cancer researchers use the MAS-Seq for 10x Single Cell 3’ kit (now the Kinnex single-cell RNA kit) to identify driver mutations and trace clonal populations over time in a patient’s research sample who progressed from MDS (Myelodysplastic syndrome) to AML (acute myeloid leukemia).
Explore how changes in isoform usages contribute to phenotypic differences between health and disease.
Plant + animal
Advance breeding programs and basic research with full-length cDNA sequencing that does not require a reference genome.
“Long-read sequencing results in more accurate measurements than any obtained from short-read RNA-seq to determine the presence or absence of specific isoforms.”
Schertzer et al., Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox, bioRxiv (2023)
The right solution for your RNA applications
|Application||Dataset||Download literature||Technology||Sequencing system|
|Kinnex single-cell||Homo sapiens - PBMC 10x Chromium Single Cell 3' libraries||Kinnex for single-cell isoform sequencing||HiFi long-read||Sequel II and Revio systems|
|Kinnex full-length RNA||Homo sapiens – universal human reference RNA (UHRR)||Kinnex full-length RNA||HiFi long-read||Sequel II and Revio systems|
|Kinnex full-length RNA||Homo sapiens – HG002||Kinnex full-length RNA||HiFi long-read||Revio system|
|Kinnex 16S rRNA||ZymoBIOMICS Fecal Reference with TruMatrix Technology (human)||Coming soon||HiFi long-read||Sequel II and Revio systems|
|Kinnex 16S rRNA||Mixture: ZymoBIOMICS Gut Microbiome Standard, ZymoBIOMICS Fecal Reference with TruMatrix Technology, ATCC 20 Strain Even Mix Genomic Material, ATCC 20 Strain Staggered Mix Genomic Material||Coming soon||HiFi long-read||Sequel II and Revio systems|
Long-read RNA sequencing in action
ISO-SEQ METHOD DIFFERENTIATES PSEUDOGENES ASSOCIATED WITH DISEASES
Read how researchers at University College London used the Iso-Seq method to accurately map and characterize the parent-pseudogene pair GBA-GBAP1 which is implicated in Gaucher’s disease and Parkinson’s disease.
Isoform-resolution single-cell atlas of colorectal cancer
Researchers apply long reads to single-cell RNA-Seq in colorectal cancer to identify recurring neoepitopes as potential cancer vaccine candidates.
ISO-SEQ METHOD OUTPERFORMS OTHER LONG-READ METHODS IN BENCHMARKETING CONSORTIUM STUDY
The LRGASP consortium published their assessment of long-read RNA sequencing using different sequencing platforms and analysis tools.
PROTEIN ISOFORM DISCOVERY ENHANCED BY ISOFORM SEQUENCING
Researchers use Iso-Seq data to predict open reading frames integrated into a proteogenomics workflow to discover novel protein isoforms to reveal the functional impacts of alternative splicing.
FULL-LENGTH RNA SEQUENCING SPOTLIGHT
The Kinnex full-length RNA kit produces full-length isoforms up to 10 kb. Combine with the Iso-Seq express 2.0 kit and SMRT Link analysis to characterize and compare known and novel isoforms for your RNA research.