RNA sequencing with the Iso-Seq method
Reveal novel biology that other sequencing methods miss with full-length transcripts using the Iso-Seq™ method
Human transcriptome studies
Explore how changes in isoform usages contribute to phenotypic differences between health and disease.
Plant + animal transcriptome studies
Advance breeding programs and basic research with full-length cDNA sequencing that does not require a reference genome.
Single-cell transcriptome studies
Visualize biology at an even higher resolution by capturing a comprehensive and complete transcript sequence from individual cells.
Full-length RNA sequencing using the Iso-Seq method
With long and accurate HiFi reads, you can characterize the full diversity of the transcriptome:
- See alternative start and end sites
- Characterize splicing events
- Identify allele-specific isoforms
- Profile expression at isoform resolution
- Detect fusion genes
- Predict full-length open read frames
- …and more
“More than 50% of isoforms robustly detected using Iso-Seq could not be readily recapitulated using standard RNA-seq, highlighting the advantage of long-read sequencing for characterizing isoform diversity.”1
Leung, et al. (2021) Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing. Cell Rep. 37(7):110022.
RNA sequencing with Iso-Seq analysis in action
Article
ISO-SEQ METHOD DIFFERENTIATES PSEUDOGENES ASSOCIATED WITH DISEASES
Read how researchers at University College London used the Iso-Seq method to accurately map and characterize the parent-pseudogene pair GBA-GBAP1 which are implicated in Gaucher disease and Parkinson’s disease
Scalable RNA isoform sequencing using intra-molecular multiplexed cDNAs
For scalable full-length RNA sequencing, we have developed multiplexed arrays sequencing…
INNOVATIONS ABOUND AT ISO-SEQ SOCIAL CLUB VOL. 3
Read the recap on the latest Iso-Seq research that is pushing the frontiers of cutting-edge science
PROTEIN ISOFORM DISCOVERY ENHANCED BY ISOFORM SEQUENCING
Researchers use Iso-Seq data to predict open reading frames integrated into a proteogenomics workflow to discovery novel protein isoforms to reveal the functional impacts of alternative splicing
EXPLORE
Did you know we have a comprehensive library of articles, reports, papers, and videos related to RNA sequencing with the Iso-Seq method?
SINGLE-CELL RNA SEQUENCING SPOTLIGHT
MAS-SEQ KIT DELIVERS 16x THROUGHPUT FOR SINGLE-CELL ISOFORM SEQUENCING
The MAS-Seq for 10x Single Cell 3’ kit (based on the MAS-Seq method by Al’Khafaji et al.) takes advantage of long and accurate HiFi read lengths to increase single-cell Iso-Seq throughput by 16-fold through concatenated arrays of single-cell cDNA molecules.
Learn more about the MAS-Seq kit now at the single-cell Iso-Seq webpage.
Iso-Seq application workflows at a glance
Advantages of RNA sequencing with the
Iso-Seq method
Powered by HiFi reads, the Iso-Seq method uncovers biology that other approaches miss.
Iso-Seq Method delivers full-length transcript isoforms, no assembly required
Unlike short-read RNA-seq methods, Iso-Seq method reads span the whole transcript, capturing even untranslated regions.
- Detect alternative polyadenylation
- Detect differential isoform usages (DIU)
- Obtain isoform-level phasing information
Iso-Seq Data is comprehensive
Characterize whole transcriptome isoform diversity that can reveal complex alternative splicing, fusion events, and transcriptional readthroughs.
Iso-Seq Analysis offers unparalleled insight at the single cell level
Use any single-cell platform to generate full-length cDNA as input for SMRT sequencing and achieve all the same benefits of Iso-Seq on bulk cDNA — complete transcript sequence, isoform-level phasing, and isoform profiling — at single-cell resolution.
Common questions about PacBio Iso-Seq method and RNA sequencing
SHAPING THE FUTURE
Iso-Seq analysis provides insights into feats of physiology of hibernating bears
Washington State University researchers use Iso-Seq analysis to shed light on the metabolic mystery of brown bears.
FEATURED LONG-READ SEQUENCING SYSTEMS
Achieve highly accurate full-length cDNA sequencing with PacBio long-read sequencers.