Sequel systems

The PacBio Sequel II and IIe systems provide scientists with access to cost effective and highly accurate long-read sequencing.

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An introduction to the Sequel II systems

Watch this short video to learn how the Sequel II and IIe systems can help you be confident in your discoveries made possible with comprehensive views of genomes, transcriptomes, and epigenomes.

KEY DIFFERENCES BETWEEN HIFI LONG-READ SYSTEMS

If you’re exploring long-read sequencing, consider Vega and Revio systems. They are designed for flexibility, quality, and performance, they make it easier than ever to access the gold standard in HiFi long-read sequencing. Download HiFi systems brochure to learn more.

System specs	Vega system	Revio system	Sequel II and IIe systems
Input amount per SMRT Cell	2 µg	500 ng with SPRQ chemistry	1 ug
Run times	12 and 24-hours	12, 24, and 30-hours	12 to 30 hours
SMRT Cells per run	1	1 to 4	1
Yield per run	60 Gb	120 to 480 Gb	30 Gb
Annual throughput	200 Vega SMRT Cells	1,300 Revio SMRT Cells	n/a
Methylation caller	5mC and 6mA	5mC and 6mA	5mC
	Discover Vega	Explore Revio

Note: Sequel IIe system sales end November 28, 2025; Sequel II is no longer available for sale. Support for both Sequel II and IIe systems ends September 30, 2028.

How HiFi sequencing compares

	HiFi sequencing	SBS sequencing	Nanopore sequencing
Read length	1–25 kb	2x150 bp	10–100 kb
Read accuracy	99.95% (Q33)	99.92% (Q31)	99.26% (Q21)
Run time	24 hours³	44 hours	72 hours
Yield	90 Gb^2,5	2,400–3,000 Gb	50–110 Gb
Variant calling — SNVs	✓	✓	✓
Variant calling — indels	✓	✓	X
Variant calling — SVs	✓	X	✓
5mC methylation	✓	X	✓
Phasing	✓	X	✓

²HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0.
³Run time specification is for the sequencing reaction.
⁵HiFi yield is dependent on library fragment size. Yield is typically lower for shorter libraries.