Sequencing systems
Access some of the world’s most advanced sequencing technologies.
Introduction to PacBio sequencing systems
Building on years of cutting-edge research and development, our systems enable researchers to make discoveries that will change the world. Explore the exceptional accuracy of our long-read and short-read sequencing systems.
REVIO SYSTEM
Long-read sequencing
Highly accurate HiFi sequencing for more complete views of genomes, epigenomes, and transcriptomes
Throughput to run up to 1,300 human HiFi genomes per year
Smart consumables for simple handling and less plastic waste
Powerful compute with Google Health DeepConsensus onboard
![]()
ONSO SYSTEM
Short-read sequencing
Up to 15x higher accuracy than other benchtop sequencers
Reduced requirement for sequencing coverage depth versus SBS sequencers
Resolve homopolymer and difficult to sequence regions
Low duplication rate, negligible index hopping
Rapid conversion of existing P5/P7 libraries for sequencing on the Onso system
![]()
SEQUEL IIe SYSTEM
Long-read sequencing
Long, accurate HiFi reads with DNA methylation direct from the instrument in every run
Supports a wide range of applications – targeted sequencing, RNA sequencing, and whole-genome sequencing
Throughput match for microbial genome sequencing, AAV vector sequencing, Iso-Seq method, and more
Featured in hundreds of peer-reviewed publications
“Using PacBio sequencing we can detect mutations that otherwise would be missed in Illumina sequencing”
– van Schendel, NAR Genomics and Bioinformatics, 2022
GAME, CHANGED.
Meet Revio and Onso — Our newest long-read and short-read sequencing systems. Long reads at a lower cost and higher throughput. Short reads with exceptional accuracy. Endless opportunities for discovery.
Your moment is waiting. What will you find?
Sequencing in action
Video
HiFi sequencing — Unlock your next great discovery
Researchers are fueling discovery with the advantages of short reads and traditional long reads in one easy-to-use technology.
SBB sequencing
The accuracy of SBB has the potential to advance cancer research, and broadly enable translational applications where sequencing quality is critical for unlocking new discoveries.
THE COMPLETE SEQUENCE OF A HUMAN GENOME
The T2T Consortium recently accomplished a landmark achievement by generating a complete human genome for the very first time — all with the help of PacBio HiFi sequencing.
How far we’ve come part one: insect genomics
The Sequel II and IIe platforms benefit entomologists even further by facilitating the sequencing of even the smallest species with as little as 5 ng of genomic DNA.
Explore
PacBio has over 10,000 articles, reports, papers, and videos related to genomic sequencing