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Discovery is in the details:
move beyond draft genomes

With SMRT Sequencing on the Sequel System, you no longer have to sacrifice quality for affordability when assembling bacterial genomes. Go well beyond draft-quality to achieve reference-quality assemblies, closing even the most repeat-dense and GC-rich genomes and resolving plasmids.

Complete microbial genomes with ease and confidence

For most microbes, closed genomes with accessory plasmids can be assembled with 50-fold coverage of SMRT Sequencing data using the default settings of our assembly pipeline.

  • Generate platinum-standard, closed reference genomes
  • Affordably assemble gold-standard genomes by multiplexing up to 16 microbes in one SMRT Cell
  • Clarify the role of transposons, phage insertions, and other structural variants in the evolution of virulence
  • Recover plasmids to track drug resistance and transmission paths

Workflow: from DNA to characterized microbial genomes in a single experiment

 

Sample & Template Preparation
Use SMRTbell Template Prep 1.0 and Barcoding Adapter Kits 8A/B to pool of up to 30 Mb of multiplexed microbial genomes, inclusive of any plasmids

Learn More


Sequencing
Generate whole genome sequencing data on up to 16 microbes in a single 10-hour run on one SMRT Cell 1M on the Sequel System.

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Data Analysis
Easily assembly most microbial genomes into 5 contigs or fewer (excluding plasmids) and characterize methylation with PacBio analytical software tools.

Learn More

Spotlight: Closed genomes reveal all the variants that drive microbial biology

Researchers from leading German institutes used SMRT Sequencing to obtain closed chromosomes and plasmids of Klebsiella pneumoniae ST147 isolates from hospitals, revealing that both SNPs and complex rearrangements contribute to the evolution of antibiotic resistance and virulence. Explore this research further:

Zautner, A. E., et al. (2017). Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT Sequencing. Journal of Antimicrobial Chemotherapy, 72(10), 2737–2744.

Spotlight: Gapless assemblies to better understand fungal genomes

A recent study highlighted the potential public health risk posed by historically poor fungal genome assemblies. With PacBio sequencing, it is now possibly to achieve finished yeast genomes, including complete centromeres and telomeric regions. Explore this research further:

Douglass, A. P., et al. (2018). Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names. PLoS Pathogens, 14(7), e1007138.

To learn more about PacBio’s true whole genome sequencing for microbiology and infectious disease research, contact us.

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