X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
Contact:

Discovery is in the details:
move beyond draft genomes

With SMRT Sequencing on the Sequel System, you no longer have to sacrifice quality for affordability when assembling bacterial genomes. Go well beyond draft-quality to achieve reference-quality assemblies, closing even the most repeat-dense and GC-rich genomes and resolving plasmids.

Complete microbial genomes with ease and confidence

For most microbes, closed genomes with accessory plasmids can be assembled with 50-fold coverage of SMRT Sequencing data using the default settings of our assembly pipeline.

  • Generate platinum-standard, closed reference genomes
  • Affordably assemble gold-standard genomes by multiplexing up to 16 microbes in one SMRT Cell
  • Clarify the role of transposons, phage insertions, and other structural variants in the evolution of virulence
  • Recover plasmids to track drug resistance and transmission paths

Workflow: from DNA to characterized microbial genomes in a single experiment

 

Library Preparation

 

 

SMRT Sequencing with the Sequel System

 

 

 

Data Analysis with SMRT Analysis, PacBio DevNet and our Analytical Partners

 

Spotlight: Gapless assemblies to better understand fungal genomes

A recent study highlighted the importance of complete genomes to understand effector and co-regulated genes as well as the role of centromeres and telomers in adaptive genome evolution in fungal species. Explore this research further:

Thomma, B.P.H.J. et al., 2016. Mind the gap; seven reasons to close fragmented genome assemblies. Fungal Genetics and Biology, 90, pp.24–30.

Spotlight: Resolve GC-rich microbial genomes with SMRT Sequencing

Scientists used SMRT Sequencing to resolve the genome of Burkholderia pseudomallei and compared their assembly results with hybrid and short-read assemblies. Their findings concluded that PacBio long-read sequencing generated the highest-quality, cost-effective assembly and enabled closure of a complex microbial genome. Explore this research further:

Teng, J.L. et al., 2017. PacBio but not Illumina technology can achieve fast, accurate and complete closure of the high GC, complex Burkholderia pseudomallei two-chromosome genome. Frontiers in Microbiology, 8, p.1448.

To learn more about PacBio’s true whole genome sequencing for microbiology and infectious disease research, contact us.

Selected Resources