Template preparation reagents for preparing and multiplexing SMRTbell library templates for long-read sequencing on the PacBio Sequel System.
Template preparation consumables
Quickly create libraries for Single Molecule, Real Time (SMRT) Sequencing with our template preparation kits. DNA is treated to ensure the removal of any DNA damage, and ends are repaired and circularized with the addition of SMRTbell adapters. The result is a circularized template, called a SMRTbell library.
SMRTbell templates provide a segment of single-stranded DNA to which a sequencing primer is annealed. A polymerase is bound to a primer-annealed template and the complex is ready to be loaded onto SMRT Cells.
SMRTbell Express Template Prep KitPN: 101-357-000
Quickly prepare long-insert SMRTbell libraries for the Sequel System.
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SMRTbell Template Prep Kit 1.0-SPv3PN: 100-991-900
Prepare primer-annealed, symmetric SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb.
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AMPure PBPN: 100-265-900
Magnetic beads specifically formulated for the removal of 1) small template fragments during the template preparation or 2) excess polymerase and primer from polymerase-bound SMRTbell complexes.
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Multiplexing consumables
Sequence multiple samples on the same SMRT Cell to increase throughput and enhance the efficiency of your research. Multiplexing is supported with three barcoding options providing flexibility to incorporate unique sample identifiers during target amplification or library preparation.
- Add barcoded primers to amplicons during (1) PCR through either target-specific primers with incorporated barcodes or (2) a two-step PCR using barcoded universal primers.
- Add barcodes to samples with (3) ligation of barcoded SMRTbell adapters to template.
Learn more about Multiplexing, including information on barcode sequences compatible with SMRT Sequencing.
Barcoded Adapter Kit -8A/BPN: 101-081-300/400
Two sets of 8 barcoded adapters for multiplexing SMRTbell libraries in applications such as multiplexing for microbial whole genome sequencing.
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Barcoded Universal F/R Primers Plate -96PN: 100-466-100
A set of 96 barcoded primer pairs for multiplexing amplicons containing universal sequence tags.
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SMRTbell Barcoded Adapter Complete Prep -96PN: 100-514-900
A set of 96 barcoded SMRTbell adapters for multiplexing amplicons through ligation to generate SMRTbell libraries and do not require modifications to existing, pre-designed PCR primers.
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SMRTbell Damage Repair Kit -SPv3PN: 100-992-200
Perform DNA damage repair, Exonuclease III and VII treatments, and primer annealing of SMRTbell libraries prepared with SMRTbell barcoded adapters.
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Find information on Sample and Preparation Kits for PacBio RS II