April 20, 2022  |  Technical note

Technical note — Covaris-g TUBE gDNA shearing for SMRTbell prep kit 3.0

PacBio recommends that DNA shearing for whole genome sequencing be performed on the Diagenode Megaruptor 3 system, which is the only system validated by PacBio for shearing, in order to produce consistent HiFi yield and quality. When a Megaruptor 3 system is unavailable, the Covaris g-TUBE presents an alternative shearing method that does not require instrumentation beyond a standard microcentrifuge. This Technical note provides guidelines for using the Covaris g-TUBE for DNA shearing.

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