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Application Brief: Long-read RNA sequencing – Best Practices.

2018

Summary +

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and affordably sequence transcript isoforms of up to 10 kb in their entirety. The Iso-Seq method allows users to generate full-length cDNA sequences - with no assembly required - in order to confidently characterize the full complement of transcript isoforms within targeted genes, or across an entire transcriptome.

Application Brief: Low-coverage, long-read whole genome sequencing for structural variation – Best Practices.

2018

Summary +

With the Sequel System, you can affordably and sensitively characterize structural variation (SV) of all types ranging in size from tens to thousands of base pairs. Low-coverage, long-read whole genome sequencing (WGS) data provides rapid discovery of common SVs for population genetics studies and resolves rare SVs unique to an individual, with a very low false-discovery rate.

Application Brochure: No-Amp targeted sequencing.

2018

Summary +

Due to technology limitations, repeat-expansion disorders have gone without the needed base-level resolution of the disease causative long repetitive elements. Enrichment of these hard-to-amplify genomic regions is now possible with our no-amplification (No-Amp) targeted sequencing method utilizing the CRISPR/Cas9 system.

De Novo Assembly Brochure: Assembly Options for your SMRT Sequencing Data

2018

Summary +

De novo assembly using PacBio SMRT Sequencing is now the gold standard for generating contiguous, highly accurate reference genomes. The challenge of assembly is eased with PacBio long reads, and a wealth of bioinformatics tools have been developed and optimized for SMRT Sequencing. Whether you’re a genome expert who likes to run tools at the command line or you’d prefer assembly experts to deliver a polished assembly directly to you, PacBio has a solution to fit your needs.

Case Study: Pioneering a pan-genome reference collection

2018

Summary +

At DuPont Pioneer, DNA sequencing is paramount for R&D to reveal the genetic basis for traits of interest in commercial crops such as maize, soybean, sorghum, sunflower, alfalfa, canola, wheat, rice, and others. They cannot afford to wait the years it has historically taken for high-quality reference genomes to be produced. Nor can they rely on a single reference to represent the genetic diversity in its germplasm.

Whitepaper: Structural Variation in the Human Genome

2017

Summary +

Structural variation accounts for much of the variation among human genomes. Structural variants of all types are known to cause Mendelian disease and contribute to complex disease. Learn how long-read sequencing is enabling detection of the full spectrum of structural variants to advance the study of human disease, evolution and genetic diversity.

Case Study: SMRT sequencing brings clarity to HIV vaccine and transplant research at the Wisconsin national primate research center.

2017

Summary +

The Wisconsin National Primate Research Center (WNPRC) is a leading Major Histocompatibility Complex (MHC) typing lab that focuses on monkeys. While many scientists are familiar with the importance of characterizing the histocompatibility region of the human genome for applications like disease research or tissue typing before organ transplantation, fewer are aware of the need to accurately type this region in non-human primates. At the primate research lab, part of the University of Wisconsin- Madison, scientists are analyzing immune regions to help test potential HIV vaccines and AIDS therapies. Their work is essential for understanding the effects of treatment ahead of human trials.

Plant and Animal Sciences Brochure: A comprehensive view of genetic diversity

2017

Summary +

Single Molecule, Real-Time (SMRT®) Sequencing combines long reads with uniform coverage to provide uniquely comprehensive views of plant and animal genomes and transcriptomes. High-quality genome assemblies and evidence-based annotations promote improved genetic marker development, discovery of novel genes, and structural variation characterization.

Application Brochure: Microbial Communities – Complex microbial populations simplified.

2017

Summary +

The ability to identify and understand the functions of the complex microbial populations living in, on, and around us requires comprehensive characterization of each community member. Long reads, high accuracy, and single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, long-read metagenomic profiling, and shotgun metagenomic assembly.