June 9, 2022  |  Sequencing methods

HiFi Target Enrichment — Best Practices

With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.​


May 26, 2022  |  Products, procedures + protocols

HIFI WGS at scale on the Sequel IIe system

Scale your whole genome sequencing (WGS) and epigenome workflows with PacBio® HiFi reads employing a single technician working one day a week. Sequence 25 human genomes at ≥ 30-fold coverage each month using four Sequel® II systems.*


May 16, 2022  |  Gene therapy + editing

Application Brochure AAV Sequencing with HiFi Reads

With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation


April 20, 2022  |  Products, procedures + protocols

Application Brief – Measuring DNA methylation with 5-base HiFi sequencing

HiFi sequencing produces long, accurate reads of the 4 DNA bases — A, C, G, and T — that deliver the most comprehensive characterization of genomes. But HiFi sequencing is not limited to characterizing the genome. It simultaneously measures the epigenome by detecting a fifth base — 5mC at CpG sites — without requiring special library preparation like bisulfite treatment. This detects distinct regional epigenetic patterns, accesses methylation in the full genome, and identifies allele-specific methylation.


April 7, 2022  |  Cancer research

Application Brief: Uncover Cancer-Specific RNA Isoforms Using Long Read Sequencing

The Iso-Seq method utilizes long-read transcript sequencing to reliably capture full-length transcript isoforms without the need for
computational assembly. PacBio’s long-read RNA sequencing is also significantly less error-prone than other long-read sequencing
technologies, offering more robust and accurate isoform discovery power.9,10 Future research will continue to explore the complex and
mostly unexplored landscape of isoforms across cancer types.


August 19, 2021  |  Sequencing methods

Application Brief: Targeted sequencing for amplicons – Best Practices

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.


August 19, 2021  |  Sequencing methods

Application Brief: Long-read RNA sequencing – Best Practices

With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can easily and affordably sequence complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required — to confidently characterize full-length transcript isoforms.


August 19, 2021  |  Sequencing methods

Application Brief: Single-cell RNA sequencing with HiFi reads – Best Practices

With PacBio single-cell RNA sequencing using the Iso-Seq method, you can now distinguish between alternative transcript isoforms at the single-cell level. The highly accurate long reads (HiFi reads) can span the entire 5′ to 3′ end of a transcript, allowing a high-resolution view of isoform diversity and revealing cell-to-cell heterogeneity without the need for assembly.


August 19, 2021  |  Human genetics research

Application Brochure: Gene editing validation with HiFi reads

With highly accurate long reads (HiFi reads) from the Sequel IIe System, powered by Single Molecule, Real-Time (SMRT) Sequencing technology, you can efficiently and cost effectively validate gene editing techniques including adeno-associated virus (AAV) and CRISPR-Cas9 approaches.


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