April 11, 2024 | Microbial sequencing methods
Asset Type: Application brochures and briefs
December 20, 2023 | Cancer research
Application brief — HiFi sequencing enables greater accuracy of somatic variant calling
December 11, 2023 | Gene therapy + editing
Application brief — Highly-accurate HiFi reads gene editing
December 4, 2023 | Gene therapy + editing
Application brief — Highly-accurate HiFi reads for AAV-research
October 18, 2023 | Products, procedures + protocols
Application brief — Germline variant detection with the Onso system
August 24, 2023 | Products, procedures + protocols
Application brief — What can you do with a Revio system
August 8, 2023 | Cancer research
Application brief — Superior sensitivity for ctDNA detection on the Onso System
June 22, 2023 | Microbial sequencing methods
Application brief — Taxonomic and functional profiling with HiFi metagenomics
March 17, 2023 | Targeted sequencing
Application brief — Scalable HiFi sequencing with Twist Bioscience long-read alliance panels
June 9, 2022 | Sample + library preparation
Application brief — HiFi target enrichment with Twist probes
With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.
May 26, 2022 | Products, procedures + protocols
Application brief — HIFI WGS at scale on the Sequel IIe system
Scale your whole genome sequencing (WGS) and epigenome workflows with PacBio® HiFi reads employing a single technician working one day a week. Sequence 25 human genomes at ≥ 30-fold coverage each month using four Sequel® II systems.*
May 16, 2022 | Gene therapy + editing
Application brochure — AAV Sequencing with HiFi Reads
With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation
April 20, 2022 | Products, procedures + protocols
Application brief — Measuring DNA methylation with 5-base HiFi sequencing
HiFi sequencing produces long, accurate reads of the 4 DNA bases — A, C, G, and T — that deliver the most comprehensive characterization of genomes. But HiFi sequencing is not limited to characterizing the genome. It simultaneously measures the epigenome by detecting a fifth base — 5mC at CpG sites — without requiring special library preparation like bisulfite treatment. This detects distinct regional epigenetic patterns, accesses methylation in the full genome, and identifies allele-specific methylation.
April 7, 2022 | Cancer research
Application brief — Uncover cancer-specific RNA isoforms using long read sequencing
The Iso-Seq method utilizes long-read transcript sequencing to reliably capture full-length transcript isoforms without the need for
computational assembly. PacBio’s long-read RNA sequencing is also significantly less error-prone than other long-read sequencing
technologies, offering more robust and accurate isoform discovery power.9,10 Future research will continue to explore the complex and
mostly unexplored landscape of isoforms across cancer types.