December 13, 2023
Where can I find the most recent protocols for long-read sequencing?
You can find the protocols (procedure & checklists) on the protocols page (filtered view from featured documentation).
Workflow Step: Sequencing
December 13, 2023
Why do I get an error [Missing Reference Annotation] in SMRT Link run design when setting up a run?
Before running applications that require reference annotation (selecting Kinnex in run design) for the first time, a reference annotation must be added to SMRT Link or an error is thrown…
December 13, 2023
How does SMRT sequencing work? What is a HiFi read?
HiFi reads are produced using circular consensus sequencing (CCS) mode on PacBio long-read systems. HiFi reads provide base-level resolution with 99.9% single-molecule read accuracy. HiFi reads can be used across…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
Can I use PacBio sequencing systems for targeted sequencing?
Yes, we offer PureTarget panels for PCR free targeted sequencing, hybrid capture workflows with our partners, and amplicon sequencing. Visit the targeted sequencing page for further information.
December 13, 2023
Do I need to purchase your software to analyze my data?
No purchase is required. PacBio software is free and available from our Software & Bioinformatics webpage, Bioconda, DockerHub, http://Quay.io , and the PacBio GitHub. Depending on your analysis needs, you…
December 13, 2023
How much DNA or cDNA do I need to do HiFi sequencing?
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…