Workflow Step: Sequencing
March 25, 2024
Technical overview — PureTarget repeat expansion panel library preparation using PureTarget kit
January 10, 2024
Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing
December 18, 2023
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
December 18, 2023
What is the most important step when you install SMRTLink on a cluster HPC environment?
The most critical step is ensuring that all compute nodes have access to the $SMRT_ROOT directory via an NFS mount. This shared access is essential for SMRT Link to run…
December 18, 2023
Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing
December 14, 2023
Do my primers need to be 5′ phosphorylated for amplicon sequencing?
No. Your primers do not need to be 5′ phosphorylated for amplicon sequencing.
December 14, 2023
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices.
December 14, 2023
What is the sequence of our internal control?
Contact support@pacb.com and we will provide the FASTA file.
December 13, 2023
Can I get methylation data from amplified DNA?
No. DNA amplification removes base modifications, so methylation information is lost. To detect methylation accurately, use native DNA, which preserves base modifications for analysis
December 13, 2023
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…