December 13, 2023

How much DNA or cDNA do I need to do HiFi sequencing?

For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0
For 16S, see page 7 of the following: Procedure & checklist – Preparing Kinnex libraries from 16S rRNA amplicons
For other amplicons, see page 3 of the following: Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0
For bulk mRNA, see page 1 of the following: Procedure & checklist – Preparing Kinnex libraries using the Kinnex full-length RNA kit
For single-cell cDNA, see page 1 of the following: Procedure & checklist – Preparing Kinnex libraries using Kinnex single-cell RNA kit

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