April 7, 2020  |  Products, procedures + protocols

Sequencing 101: video introduction to PacBio sequencing and the Sequel II system

We’re pleased to release a short video describing PacBio sequencing and our latest platform, the Sequel II system. If you’ve ever wondered how Single Molecule, Real-Time (SMRT) sequencing works, what the Sequel II system is, and what applications are available, this video is a great place to start.
We are excited to share the capabilities of our Sequel II system as it makes SMRT sequencing affordable for scientists in any lab and provides comprehensive views of genomes, transcriptomes, or epigenomes. The Sequel II system also produces highly accurate long reads, known as HiFi reads, to deliver the highest quality sequencing data.

The three-minute introductory video outlines the key advantages of SMRT sequencing:

  • Long reads allow you to readily assemble complete genomes and sequence full-length transcripts
  • High accuracy provides over 99.99% accurate sequencing results
  • Uniform coverage enables sequencing through regions inaccessible to other technologies
  • Single-molecule resolution lets you capture sequence data with over 99% single-molecule accuracy
  • Epigenetics that can be explored through direct detection of base modifications during sequencing

Watch the video for more information about the science behind SMRT sequencing. From the difference between circular consensus sequencing (CCS) to generate HiFi reads and continuous long read (CLR) sequencing mode, to how it makes a difference for applications such as whole genome sequencing for de novo assembly, variant detection, and RNA sequencing.

If this introductory video piqued your interest, you can learn more about the Sequel II system, the advantages of SMRT sequencing, or explore our resource library to learn how scientists worldwide are using SMRT sequencing to advance their science.


Explore other posts in the Sequencing 101 series:
The evolution of DNA sequencing tools
Why are long reads important for studying viral genomes?
Looking beyond the single reference genome to a pangenome for every species
Understanding accuracy in DNA sequencing
From DNA to discovery — the steps of SMRT sequencing
The value of sequencing full-length RNA transcripts
DNA extraction — tips, kits, and protocols

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