Delivering highly accurate long reads to drive discovery in life science
Single Molecule, Real-Time (SMRT) Sequencing is the core technology powering our long-read sequencing platforms. This innovative approach was the first of its kind and is now a proven technology used in all fields of life science. Explore each of the SMRT Sequencing Advantages below.
| Long Reads With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. |
Explore the benefits of long reads
| High Accuracy Sequencing free of systematic error achieves >99.999% consensus accuracy. |
Learn more about high accuracy
| Uniform Coverage No bias based on GC content means you can sequence through region inaccessible to other technologies. |
Discover how uniform coverage lets you see more
| Single-Molecule Resolution Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99% single-molecule accuracy. |
See the difference single-molecule resolution makes
| Epigenetics With no PCR amplification step, base modifications are directly detected during sequencing. |
Get more from your sequencing with epigenetics
The 2019 HiFi for All SMRT Grant Program is Now Open!
Sequence All Life with Confidence Using HiFi Reads
Tell us how highly accurate long reads (HiFi reads) will drive new discoveries in your research for a chance to win free sequencing.
SMRT Sequencing enables simultaneous collection of data from millions of wells using the natural process of DNA replication to sequence long fragments of native DNA or RNA.
Learn how to go from sample to sequence with this infographic.
Original Publication: Eid, J., et al. (2009). Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.
Explore the full range of SMRT Sequencing applications.
 | Whole Genome Sequencing For humans, plants, animals and microbes including de novo assembly and variant detection |  | Complex Populations Understand variants among bacterial, viral and cancer cell populations
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 | RNA Sequencing In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes |  | Epigenetics Detect DNA modifications in your samples while you sequence on the PacBio platform |
 | Targeted Sequencing Study relevant genome targets across any regions of interest |
Learn more about the Sequel II System
Contact a PacBio Certified Service Provider
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Selected Resources
- Mitsuhashi, Satomi et al. (2019) Long-read sequencing for rare human genetic diseases. Journal of human genetics
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Eichler, Evan E et al. (2019) Genetic Variation, Comparative Genomics, and the Diagnosis of Disease. The New England journal of medicine
- Mantere, Tuomo et al. (2019) Long-Read Sequencing Emerging in Medical Genetics Frontiers in genetics
- Wang, Bo et al. (2019) Reviving the Transcriptome Studies: An Insight into the Emergence of Single-molecule Transcriptome Sequencing Frontiers in genetics
- Pollard, Martin O et al. (2018) Long reads: their purpose and place. Human molecular genetics
- Sedlazeck, Fritz J et al. (2018) Accurate detection of complex structural variations using single-molecule sequencing. Nature methods
- Ardui, Simon et al. (2018) Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics. Nucleic acids research
- Nakano, Kazuma et al. (2017) Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area. Human cell
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature reviews. Genetics
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, proteomics & bioinformatics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature biotechnology
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome biology
- Travers, Kevin J et al. (2010) A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic acids research
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature methods
- Eid, John et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science