Delivering highly accurate long reads to drive discovery in life science
What is SMRT Sequencing?
Single Molecule, Real-Time (SMRT) Sequencing is the core technology powering our long-read sequencing platforms. This innovative approach was the first of its kind and is now a proven technology used in all fields of life science.
Learn how to go from DNA to discovery with SMRT Sequencing in this short video.
Original Publication: Eid, J., et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.
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What are the Advantages of SMRT Sequencing?
| Long Reads
With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. |
Explore the benefits of long reads
| High Accuracy
Sequencing free of systematic error achieves >99.999% consensus accuracy. Explore the benefits of highly accurate long-read sequencing. |
Learn more about high accuracy
| Uniform Coverage
No bias based on GC content means you can sequence through regions inaccessible to other technologies. |
Discover how uniform coverage lets you see more
| Single-Molecule Resolution
Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99.9% single-molecule accuracy. |
See the difference single-molecule resolution makes
| Epigenetics
With no PCR amplification step, base modifications are directly detected during sequencing. |
Get more from your sequencing with epigenetics
What Can You Do With SMRT Sequencing?
Explore the full range of SMRT Sequencing applications.
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Whole Genome Sequencing
For humans, plants, animals and microbes including de novo assembly and variant detection |
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Complex Populations
Understand variants among bacterial, viral and cancer cell populations |
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RNA Sequencing
In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes |
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Epigenetics
Detect DNA modifications in your samples while you sequence on the PacBio platform |
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Targeted Sequencing
Study relevant genome targets across any regions of interest |
How to Get Started with SMRT Sequencing?
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Learn more about SMRT Sequencing using the award-winning Sequel Systems |
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Connect with a PacBio scientist to have your questions answered or for a free project consultation
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Contact a PacBio Certified Service Provider to run a sequencing project from start to finish |
Selected Resources
- Mitsuhashi, Satomi et al. (2019) Long-read sequencing for rare human genetic diseases. Journal of human genetics
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Eichler, Evan E et al. (2019) Genetic Variation, Comparative Genomics, and the Diagnosis of Disease. The New England journal of medicine
- Mantere, Tuomo et al. (2019) Long-Read Sequencing Emerging in Medical Genetics Frontiers in genetics
- Wang, Bo et al. (2019) Reviving the Transcriptome Studies: An Insight into the Emergence of Single-molecule Transcriptome Sequencing Frontiers in genetics
- Pollard, Martin O et al. (2018) Long reads: their purpose and place. Human molecular genetics
- Sedlazeck, Fritz J et al. (2018) Accurate detection of complex structural variations using single-molecule sequencing. Nature methods
- Ardui, Simon et al. (2018) Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics. Nucleic acids research
- Nakano, Kazuma et al. (2017) Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area. Human cell
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature reviews. Genetics
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, proteomics & bioinformatics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature biotechnology
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome biology
- Travers, Kevin J et al. (2010) A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic acids research
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature methods
- Eid, John et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science
- Poster: Zhu, Lei et al. (2021) Full-Length Sequencing of CYP2D6Variants with PacBio HiFi Reads
- Poster: Rowell, William J. et al. (2020) Comprehensive variant detection in a human genome with highly accurate long reads
- Poster: Rowell, William J. et al. (2020) A workflow for the comprehensive detection and prioritization of variants in human genomes with PacBio HiFi reads
- Poster: Portik, Daniel et al. (2020) Metagenomic analysis of type II diabetes gut microbiota using PacBio HiFi reads reveals taxonomic and functional differences
- Poster: Wenger, Aaron M. et al. (2020) Copy-number variant detection with PacBio long reads
- Poster: Ashby, Meredith et al. (2020) Unbiased characterization of metagenome composition and function using HiFi sequencing on the PacBio Sequel II System
- Poster: Tseng, Elizabeth et al. (2020) A complete solution for high-quality genome annotation using the PacBio Iso-Seq method
- Poster: Galvin, Brendan et al. (2020) A high-quality PacBio insect genome from 5 ng of input DNA
- Poster: Kingan, Sarah B. et al. (2020) Beyond Contiguity: Evaluating the accuracy of de novo genome assemblies
- Poster: Kingan, Sarah B. et al. (2020) Every species can be a model: Reference-quality PacBio genomes from single insects
- Ashby, Meredith; Bickhart, Derek, and Portik, Dan (2021) Revealing Mechanisms of Bacterial Virulence and Adaptation with PacBio SMRT Sequencing
- Hiatt, Susan and Korlach, Jonas (2021) Long-Read Genome Sequencing for the Molecular Understanding of Neurodevelopmental Disorders
- Ashby, Meredith and Brown, Candice and Driscoll, Mark and Leung, Elaine (2021) Identifying Key Players in Host-Microbiome Interactions with High Resolution 16S Sequencing
- Ashby, Meredith; and Boritz, Eli (2021) Resolving Viral Evolution and Quasispecies Diversity with HiFi Sequencing
- Ashby, Meredith; Bickhart, Derek, and Portik, Dan (2021) Optimizing for Information: What Richer Data and Better Assemblies Reveal About Metagenome Structure and Function
- Tai, Phillip (2021) AAV-Genome Population Sequencing of Vectors Packaging CRISPR Components Reveals Design-Influenced Heterogeneity
- Joglekar, Anoushka (2021) A Spatially Resolved Brain Region and Cell Type-Specific Isoform Atlas of the Postnatal Mouse Brain
- Levy, Shawn and Heiner, Cheryl and Aro, Lori (2021) Transformative Drug Research and Development via Accurate and Scalable Long-Read Sequencing Services
- Aro, Lori and Scott, Stuart A and Yang, Yao (2021) Advancing Pharmacogenomics Research and the Need for Highly Accurate Long-Read Sequencing
- Blethrow, Justin and Smith, Melissa L. and Vinnere Pettersson, Olga (2021) Webinar: Unleashing the Power of HiFi Sequencing – How the Sequel IIe System Removes Barriers and Empowers Life Scientists
- Miller, Dave and Hernandez, Alvaro (2021) Creating Core Demand with HiFi Sequencing
- Wenger, Aaron (2021) Not All Long Reads Are Equal – High Accuracy Sets PacBio Apart
- Miller, David and Smith, Melissa Laird (2021) The Long and Short of Sequencing – Why HiFi Reads are the Future
- Becker, Aaron and Ekholm, Jenny and Harris, Charlotte and Kingham, Brewster and Vinnere Pettersson, Olga (2021) SMRT Sequencing as a Service – How to Bring Long-Read Technology to Your Core Lab
- Korlach, Jonas (2021) AGBT Presentation: Increasing the Solve Rate of Rare and Undiagnosed Genetic Diseases with HiFi Sequencing
- Wang, Bo (2021) PagBio Day: Comparative Genomic Analysis in Sorghum Highlights the Extent of Structural Variations and Sugarcane Aphid Resistance Genes
- Vierra, Michelle and Lewin, Haris (2021) PagBio Day: New Discoveries in Plant and Animal Sciences
- Schuele, Birgitt (2020) Long-read Sequencing and Optical Mapping of ATXN10 Repeat Expansion
- Holstege, Henne (2020) Uncovering Neurological Disorders Through an Examination of VNTRs
- Levandoski, Michael (2020) Webinar: Geographic and Temporal Mapping of the SARS-CoV-2 Pandemic in the United States
- (2020) Application Tutorial: Introduction to Variant Detection with HiFi Reads
- (2020) Introduction to highly accurate long-read sequencing (HiFi Sequencing)
- Chong, Tang (2020) Virtual Global Summit: HIT-scISOseq – High-throughput and high-accuracy single-cell full-length isoform sequencing
- Newell, Nicole (2020) Virtual Global Summit: Technical Tutorial – Choosing the best library prep for HiFi sequencing
- Ashby, Meredith (2020) Virtual Global Summit: The HiFi sequencing advantage for metagenome assembly
- Hernandez, Alvaro (2020) Virtual Global Summit: Using the unique capabilities of the Sequel II System for de novo genome assembly and full-length 16S sequencing
- Wenger, Aaron and Tseng, Elizabeth (2020) ASHG CoLab: PacBio HiFi reads for comprehensive characterization of genomes and single-cell isoform expression
- Korlach, Jonas (2020) ASHG PacBio Workshop: Latest product and application updates
- Chakraborty, Shreyasee and Eng, Kevin and Warner, Sarah and Lee, Walter and Madamba, Nicole and Obermoeller, Dawn and Wenger, Aaron and Weiand, Michael and Larrea, Andres and Baybayan, Primo (2020) Video Poster: High-throughput HiFi library workflow for human whole genome sequencing on the Sequel II System
- (2020) Video: HiFi Sequencing – Unlock your next great discovery
- Wenger, Aaron (2020) Educational Video: Variant Detection with HiFi Reads – Understanding Results from the precisionFDA Truth Challenge
- Geib, Scott and Laumer, Christopher and Uliano da Silva, Marcela and Vierra, Michelle (2020) Webinar: No Organism Too Small – Build high-quality genome assemblies of small organisms with HiFi sequencing
- Ameur, Adam (2020) Webinar: SMRT Sequencing applications for human genomics and medicine
- Sund, Kristen and Wenger, Aaron (2020) Webinar: Increasing solve rates for rare and Mendelian diseases with long-read sequencing
- Ashby, Meredith and Laird-Smith, Melissa and Watson, Corey (2020) Webinar: Understanding SARS-CoV-2 and host immune response to COVID-19 with PacBio sequencing
- Application Brief: Metagenomic sequencing with HiFi reads – Best Practices (2021)
- Publication at a Glance: The complete sequence of a human genome (2021)
- Informational Guide: Whole genome sequencing for understanding rare diseases (2021)
- Application Brief: Variant detection using whole genome sequencing with HiFi reads – Best Practices (2021)
- Application Brief: Whole genome sequencing for de novo assembly – Best Practices (2021)
- Application Brochure: Scalable human whole genome HiFi sequencing for rare and inherited disease research (2021)
- Core Lab Brochure: The most trusted long-read technology (2021)
- Application Brochure: Gene editing validation with HiFi reads (2021)
- SMRT Sequencing Brochure: Delivering highly accurate long reads to drive discovery in life science (2020)
- Application Brief: No-Amp targeted sequencing – Best Practices (2020)
- Product Brochure: Sequel IIe System – Sequencing evolved (2020)
- Informational Guide: Understanding accuracy in DNA sequencing (2020)
- Application Note: Considerations for using the low and ultra-low DNA input workflows for whole genome sequencing (2020)
- Application Brochure: HiFi reads for highly accurate long-read sequencing (2020)
- Technical Note: Preparing DNA for PacBio HiFi sequencing – Extraction and quality control (2020)
- Informational Guide: Looking beyond the single reference genome to a pangenome for every species (2020)
- Infographic: A Genome Fit for a Giant – Sequencing the California redwood (2020)