Delivering highly accurate long reads to drive discovery in life science
What is SMRT Sequencing?
Single Molecule, Real-Time (SMRT) Sequencing is the core technology powering our long-read sequencing platforms. This innovative approach was the first of its kind and is now a proven technology used in all fields of life science.
Learn how to go from DNA to discovery with SMRT Sequencing in this short video.
Original Publication: Eid, J., et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.
What are the Advantages of SMRT Sequencing?
| Long Reads
With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. |
Explore the benefits of long reads
| High Accuracy
Sequencing free of systematic error achieves >99.999% consensus accuracy. Explore the benefits of highly accurate long-read sequencing. |
Learn more about high accuracy
| Uniform Coverage
No bias based on GC content means you can sequence through regions inaccessible to other technologies. |
Discover how uniform coverage lets you see more
| Single-Molecule Resolution
Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99.9% single-molecule accuracy. |
See the difference single-molecule resolution makes
| Epigenetics
With no PCR amplification step, base modifications are directly detected during sequencing. |
Get more from your sequencing with epigenetics
What Can You Do With SMRT Sequencing?
Explore the full range of SMRT Sequencing applications.
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Whole Genome Sequencing
For humans, plants, animals and microbes including de novo assembly and variant detection |
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Complex Populations
Understand variants among bacterial, viral and cancer cell populations |
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RNA Sequencing
In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes |
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Epigenetics
Detect DNA modifications in your samples while you sequence on the PacBio platform |
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Targeted Sequencing
Study relevant genome targets across any regions of interest |
How to Get Started with SMRT Sequencing?
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Learn more about SMRT Sequencing using the award-winning Sequel Systems |
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Connect with a PacBio scientist to have your questions answered or for a free project consultation
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Contact a PacBio Certified Service Provider to run a sequencing project from start to finish |
Selected Resources
- Mitsuhashi, Satomi et al. (2019) Long-read sequencing for rare human genetic diseases. Journal of human genetics
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Eichler, Evan E et al. (2019) Genetic Variation, Comparative Genomics, and the Diagnosis of Disease. The New England journal of medicine
- Mantere, Tuomo et al. (2019) Long-Read Sequencing Emerging in Medical Genetics Frontiers in genetics
- Wang, Bo et al. (2019) Reviving the Transcriptome Studies: An Insight into the Emergence of Single-molecule Transcriptome Sequencing Frontiers in genetics
- Pollard, Martin O et al. (2018) Long reads: their purpose and place. Human molecular genetics
- Sedlazeck, Fritz J et al. (2018) Accurate detection of complex structural variations using single-molecule sequencing. Nature methods
- Ardui, Simon et al. (2018) Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics. Nucleic acids research
- Nakano, Kazuma et al. (2017) Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area. Human cell
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature reviews. Genetics
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, proteomics & bioinformatics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature biotechnology
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome biology
- Travers, Kevin J et al. (2010) A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic acids research
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature methods
- Eid, John et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science
- Poster: Kingan, Sarah et al. (2021) HiFiViral SARS-CoV-2: A Kitted Solution for Genome Surveillance that is Robust Across Sample Input Quantities and New Variants
- Poster: Zhu, Lei et al. (2021) Full-Length Sequencing of CYP2D6Variants with PacBio HiFi Reads
- Poster: Rowell, William J. et al. (2020) Comprehensive variant detection in a human genome with highly accurate long reads
- Poster: Rowell, William J. et al. (2020) A workflow for the comprehensive detection and prioritization of variants in human genomes with PacBio HiFi reads
- Poster: Portik, Daniel et al. (2020) Metagenomic analysis of type II diabetes gut microbiota using PacBio HiFi reads reveals taxonomic and functional differences
- Poster: Wenger, Aaron M. et al. (2020) Copy-number variant detection with PacBio long reads
- Poster: Ashby, Meredith et al. (2020) Unbiased characterization of metagenome composition and function using HiFi sequencing on the PacBio Sequel II System
- Poster: Tseng, Elizabeth et al. (2020) A complete solution for high-quality genome annotation using the PacBio Iso-Seq method
- Poster: Galvin, Brendan et al. (2020) A high-quality PacBio insect genome from 5 ng of input DNA
- Poster: Kingan, Sarah B. et al. (2020) Beyond Contiguity: Evaluating the accuracy of de novo genome assemblies
- Poster: Kingan, Sarah B. et al. (2020) Every species can be a model: Reference-quality PacBio genomes from single insects
- (2021) PacBio HiFiViral SARS-CoV-2 Kit
- Tsai, Yu-Chih (2021) A High-Resolution Panel for Uncovering Repeat Expansions that Cause Ataxias
- Kingan, Sarah (2021) HiFiViral: A Novel Method for Surveillance of SARS-CoV-2 that is Robust Across Sample Input Quantities and the Evolution of New Variants
- Wenger, Aaron (2021) Methylation Detection with PacBio HiFi Sequencing
- Gonzaludo, Nina (2021) Using HiFi Reads for Improved and Accurate Haplotyping and Phasing of Pharmacogenomic Alleles
- Henry, Christian and Shen, Richard and Stone, Jennifer (2021) With Long Reads and Short Reads, the Possibilities are Endless – PacBio ASHG 2021 Fireside Chat
- Stone, Jennifer (2021) HiFi Sequencing: See What You’ve Been Missing
- Farrow, Emily (2021) Integrated Rare Disease using Long-Read Genome Sequencing
- Al’Khafaji, Aziz (2021) Scalable RNA Isoform Sequencing using Intramolecular Multiplexed cDNAs
- Holstege, Henne (2021) Uncovering Neurological Disorders Through an Examination of VNTRs
- Ashby, Meredith and Fiske, Haley (2021) SFAF NGS Tech Panel
- Crawford, Seth and Feldmann, Mitchell and Vierra, Michelle and Ware, Doreen (2021) Getting the Most Out of Your Breeding Program with DNA and RNA Sequencing
- Lovell, John (2021) Four Chromosome Scale Genomes and a Pan-Genome Annotation to Accelerate Pecan Tree Breeding
- Kolmogorov, Mikhail (2021) Generation of Lineage-Resolved Complete Metagenome-Assembled Genomes in Complex Microbial Communities
- Ashby, Meredith; Bickhart, Derek, and Portik, Dan (2021) Revealing Mechanisms of Bacterial Virulence and Adaptation with PacBio SMRT Sequencing
- Hiatt, Susan and Korlach, Jonas (2021) Long-Read Genome Sequencing for the Molecular Understanding of Neurodevelopmental Disorders
- Ashby, Meredith and Brown, Candice and Driscoll, Mark and Leung, Elaine (2021) Identifying Key Players in Host-Microbiome Interactions with High Resolution 16S Sequencing
- Ashby, Meredith; and Boritz, Eli (2021) Resolving Viral Evolution and Quasispecies Diversity with HiFi Sequencing
- Ashby, Meredith; Bickhart, Derek, and Portik, Dan (2021) Optimizing for Information: What Richer Data and Better Assemblies Reveal About Metagenome Structure and Function
- Tai, Phillip (2021) AAV-Genome Population Sequencing of Vectors Packaging CRISPR Components Reveals Design-Influenced Heterogeneity
- Joglekar, Anoushka (2021) A Spatially Resolved Brain Region and Cell Type-Specific Isoform Atlas of the Postnatal Mouse Brain
- Levy, Shawn and Heiner, Cheryl and Aro, Lori (2021) Transformative Drug Research and Development via Accurate and Scalable Long-Read Sequencing Services
- Aro, Lori and Scott, Stuart A and Yang, Yao (2021) Advancing Pharmacogenomics Research and the Need for Highly Accurate Long-Read Sequencing
- Blethrow, Justin and Smith, Melissa L. and Vinnere Pettersson, Olga (2021) Webinar: Unleashing the Power of HiFi Sequencing – How the Sequel IIe System Removes Barriers and Empowers Life Scientists
- Miller, Dave and Hernandez, Alvaro (2021) Creating Core Demand with HiFi Sequencing
- Wenger, Aaron (2021) Not All Long Reads Are Equal – High Accuracy Sets PacBio Apart
- Miller, David and Smith, Melissa Laird (2021) The Long and Short of Sequencing – Why HiFi Reads are the Future
- Becker, Aaron and Ekholm, Jenny and Harris, Charlotte and Kingham, Brewster and Vinnere Pettersson, Olga (2021) SMRT Sequencing as a Service – How to Bring Long-Read Technology to Your Core Lab
- Korlach, Jonas (2021) AGBT Presentation: Increasing the Solve Rate of Rare and Undiagnosed Genetic Diseases with HiFi Sequencing
- Wang, Bo (2021) PagBio Day: Comparative Genomic Analysis in Sorghum Highlights the Extent of Structural Variations and Sugarcane Aphid Resistance Genes
- Vierra, Michelle and Lewin, Haris (2021) PagBio Day: New Discoveries in Plant and Animal Sciences
- Schuele, Birgitt (2020) Long-read Sequencing and Optical Mapping of ATXN10 Repeat Expansion
- Holstege, Henne (2020) Uncovering Neurological Disorders Through an Examination of VNTRs
- Levandoski, Michael (2020) Webinar: Geographic and Temporal Mapping of the SARS-CoV-2 Pandemic in the United States
- (2020) Application Tutorial: Introduction to Variant Detection with HiFi Reads
- SMRT Sequencing Brochure: Delivering highly accurate long reads to drive discovery in life science (2021)
- Application Brief: Metagenomic sequencing with HiFi reads – Best Practices (2021)
- Publication at a Glance: The complete sequence of a human genome (2021)
- Informational Guide: Whole genome sequencing for understanding rare diseases (2021)
- Application Brief: Variant detection using whole genome sequencing with HiFi reads – Best Practices (2021)
- Application Brief: Whole genome sequencing for de novo assembly – Best Practices (2021)
- Application Brochure: Scalable human whole genome HiFi sequencing for rare and inherited disease research (2021)
- Core Lab Brochure: The most trusted long-read technology (2021)
- Application Brochure: Gene editing validation with HiFi reads (2021)
- Application Brief: No-Amp targeted sequencing – Best Practices (2020)
- Product Brochure: Sequel IIe System – Sequencing evolved (2020)
- Informational Guide: Understanding accuracy in DNA sequencing (2020)
- Application Note: Considerations for using the low and ultra-low DNA input workflows for whole genome sequencing (2020)
- Application Brochure: HiFi reads for highly accurate long-read sequencing (2020)
- Technical Note: Preparing DNA for PacBio HiFi sequencing – Extraction and quality control (2020)
- Informational Guide: Looking beyond the single reference genome to a pangenome for every species (2020)
- Infographic: A Genome Fit for a Giant – Sequencing the California redwood (2020)