Delivering highly accurate long reads to drive discovery in life science
Single Molecule, Real-Time (SMRT) Sequencing is the core technology powering our long-read sequencing platforms. This innovative approach was the first of its kind and is now a proven technology used in all fields of life science.
Learn how to go from DNA to discovery with SMRT Sequencing in this short video.
Original Publication: Eid, J., et al. (2009). Real-time DNA sequencing from single polymerase molecules. Science, 323(5910), 133–138.
| Long Reads With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. |
Explore the benefits of long reads
| High Accuracy Sequencing free of systematic error achieves >99.999% consensus accuracy. |
Learn more about high accuracy
| Uniform Coverage No bias based on GC content means you can sequence through region inaccessible to other technologies. |
Discover how uniform coverage lets you see more
| Single-Molecule Resolution Capturing sequence data from native DNA or RNA molecules enables highly accurate long reads with >99% single-molecule accuracy. |
See the difference single-molecule resolution makes
| Epigenetics With no PCR amplification step, base modifications are directly detected during sequencing. |
Get more from your sequencing with epigenetics
Explore the full range of SMRT Sequencing applications.
 | Whole Genome Sequencing For humans, plants, animals and microbes including de novo assembly and variant detection |  | Complex Populations Understand variants among bacterial, viral and cancer cell populations
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 | RNA Sequencing In-depth analysis of cDNA sequences across the entire transcriptome or targeted genes |  | Epigenetics Detect DNA modifications in your samples while you sequence on the PacBio platform |
 | Targeted Sequencing Study relevant genome targets across any regions of interest |
Learn more about the Sequel II System
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Selected Resources
- Mitsuhashi, Satomi et al. (2019) Long-read sequencing for rare human genetic diseases. Journal of human genetics
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Eichler, Evan E et al. (2019) Genetic Variation, Comparative Genomics, and the Diagnosis of Disease. The New England journal of medicine
- Mantere, Tuomo et al. (2019) Long-Read Sequencing Emerging in Medical Genetics Frontiers in genetics
- Wang, Bo et al. (2019) Reviving the Transcriptome Studies: An Insight into the Emergence of Single-molecule Transcriptome Sequencing Frontiers in genetics
- Pollard, Martin O et al. (2018) Long reads: their purpose and place. Human molecular genetics
- Sedlazeck, Fritz J et al. (2018) Accurate detection of complex structural variations using single-molecule sequencing. Nature methods
- Ardui, Simon et al. (2018) Single molecule real-time (SMRT) sequencing comes of age: applications and utilities for medical diagnostics. Nucleic acids research
- Nakano, Kazuma et al. (2017) Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area. Human cell
- Chaisson, Mark J P et al. (2015) Genetic variation and the de novo assembly of human genomes. Nature reviews. Genetics
- Rhoads, Anthony et al. (2015) PacBio sequencing and its applications. Genomics, proteomics & bioinformatics
- Berlin, Konstantin et al. (2015) Assembling large genomes with single-molecule sequencing and locality-sensitive hashing. Nature biotechnology
- Huddleston, John et al. (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome research
- Koren, Sergey et al. (2013) Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome biology
- Travers, Kevin J et al. (2010) A flexible and efficient template format for circular consensus sequencing and SNP detection. Nucleic acids research
- Flusberg, Benjamin A et al. (2010) Direct detection of DNA methylation during single-molecule, real-time sequencing. Nature methods
- Eid, John et al. (2009) Real-time DNA sequencing from single polymerase molecules. Science