August 19, 2021 |
Infographic — Identify the cause of unsolved genetic disease with long-read sequencing
Explore how long-read sequencing enables solving of rare and mendelian diseases.
Auto Tag: Human
August 19, 2021 |
Infographic — SMRT Sequencing – How it works
PacBio Systems are powered by Single Molecule, Real-Time (SMRT) Sequencing, a technology proven to produce exceptionally long reads with high accuracy. SMRT Sequencing allows you to accelerate your science with the complete range of PacBio applications to produce data you can trust.
August 19, 2021 | Infectious disease research
Infographic — A brief history of microbiology
Our understanding of microbiology has evolved enormously over the last 150 years. Few institutions have witnessed our collective progress more closely than the National Collection of Type Cultures (NCTC). In fact, the collection itself is a record of the many milestones microbiologists have crossed, building on the discoveries of those who came before. To date, 60% of NCTC’s historic collection now has a closed, finished reference genome, thanks to PacBio Single Molecule, Real- Time (SMRT) Sequencing. We are excited to be their partner in crossing this latest milestone on their quest to improve human and animal health by understanding the microscopic world.
August 19, 2021 |
Infographic — Mapping the interplay of viral and host genomics with HiFi sequencing
Discover how HiFi reads enable every aspect of viral research, from understanding viral genomes to the host immune response.
August 19, 2021 |
Case Study — Scientists deconstruct cancer complexity through genome and transcriptome analysis
At Cold Spring Harbor Laboratory, scientists used SMRT Sequencing to decode one of the most challenging cancer genomes ever encountered. Along the way, they built a portfolio of open-access analysis tools that will help researchers everywhere make structural variation discoveries with long-read sequencing data.
August 19, 2021 |
Case Study — Mining complex metagenomes for protein discovery with long-read sequencing
The bacteria living on and within us can impact health, disease, and even our behavior, but there is still much to learn about the breadth of their effects. The torrent of new discoveries unleashed by high-throughput sequencing has captured the imagination of scientists and the public alike. Scientists at Second Genome are hoping to apply these insights to improve human health, leveraging their bioinformatics expertise to mine bacterial communities for potential therapeutics. Recently they teamed up with scientists at PacBio to explore how long-read sequencing might supplement their short-read-based pipeline for gene discovery, using an environmental sample as a test case. They were especially interested in identifying unique, complete, and error-free gene clusters in metagenomic assemblies.
August 19, 2021 | Cancer research
Brochure — Sequence cancer variants with confidence
To bring personalized medicine to all patients, cancer researchers need more reliable and comprehensive views of somatic variants of all sizes that drive cancer biology.
August 19, 2021 |
Case Study — Sequencing an historic bacterial collection for the future
The UK’s National Collection of Type Cultures (NCTC) is a unique collection of more than 5,000 expertly preserved and authenticated bacterial cultures, many of historical significance. Founded in 1920, NCTC is the longest established collection of its type anywhere in the world, with a history of its own that has reflected — and contributed to — the evolution of microbiology for more than 100 years.
August 19, 2021 |
Case Study — Diving Deep – Revealing the mysteries of marine life with SMRT Sequencing
Many scientists are using PacBio Single Molecule, Real-Time (SMRT) Sequencing to explore the genomes and transcriptomes of a wide variety of marine species and ecosystems. These studies are already adding to our understanding of how marine species adapt and evolve, contributing to conservation efforts, and informing how we can optimize food production through efficient aquaculture.
August 19, 2021 | Sequencing methods
Application brief — HiFi amplicon sequencing
With PacBio® long reads you can easily and cost-effectively sequence full length amplicons that target genes or regions of interest, from several hundred base pairs to kilobase scale. Highly accurate…
August 19, 2021 | Sequencing methods
Application brief — Long-read RNA sequencing
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can easily and affordably sequence complete transcript isoforms in genes of interest or across the entire transcriptome. The Iso-Seq method allows users to generate full-length cDNA sequences up to 10 kb in length — with no assembly required — to confidently characterize full-length transcript isoforms.
August 19, 2021 |
Application brochure — HiFi reads for highly accurate long-read sequencing
Discover the benefits of HiFi reads and learn how highly accurate long-read sequencing provides a single technology solution across a range of applications.
August 19, 2021 | Sequencing methods
Application brief — Single-cell RNA sequencing with HiFi reads
With PacBio single-cell RNA sequencing using the Iso-Seq method, you can now distinguish between alternative transcript isoforms at the single-cell level. The highly accurate long reads (HiFi reads) can span the entire 5′ to 3′ end of a transcript, allowing a high-resolution view of isoform diversity and revealing cell-to-cell heterogeneity without the need for assembly.
August 19, 2021 | Sequencing methods
Application brief — Whole genome sequencing for de novo assembly
PacBio HiFi reads provide both long read lengths (up to 25 kb) and high accuracy (>99.9%) to quickly and affordably generate contiguous, complete, and correct de novo genome assemblies of even the most complex genomes.
August 19, 2021 | Metagenomics
Application brief — Metagenomic sequencing with HiFi reads
Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.