Asset Tag: Plant and Animal Sciences,
Quick Reference Card — Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems
Discoveries Roadshow
After two years apart, we’ve missed you, so we’re coming to a city near you. We are visiting 20 cities across North America and Europe during April and May. Join us…
Brochure — SMRT Sequencing: Delivering highly accurate long reads to drive discovery in life science
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
Application brochure — What can you do with one SMRT Cell?
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
Procedure & Checklist — Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0
HiFi Sequencing: See What You’ve Been Missing
PacBio Vice President of Segment Marketing, Dr. Jennifer Stone, demonstrates how HiFi sequencing is changing the game in human genetics by sharing some of the exciting milestones and seminal publications…
France-Génomique & GDR Environmental and Agronomical Genomics Symposium
The Environmental and Agronomical Genomics 2021 symposium is jointly organised by France Génomique (FG) and the GDR Génomique Environnementale (GE). The Environmental and Agronomical Genomics 2021 symposium will be the opportunity to…
Getting the Most Out of Your Breeding Program with DNA and RNA Sequencing
Understanding genome sequences and how they evolved is critical for harnessing that evolutionary process for agricultural improvement. Whether asking questions about the gain/loss of genes, the role of structural variation…
Procedure & Checklist — Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0
Getting the Most Out of Your Breeding Program with DNA & RNA Sequencing
Join us for this webinar to understand the evolution of sequencing technologies and where they stand today, the experiences of three plant biologists incorporating sequencing into their work, and how…
Certified Service Provider Profile: In Seoul, DNA Link offers excellence in SMRT Sequencing
Korean service provider DNA Link has established strong expertise with the PacBio sequencing platform in response to high global demand for the technology.
Technical note: Preparing samples for PacBio whole genome sequencing for de novo assembly – Collection and storage
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in better sequencing performance across difficult to sequence regions of the genome. To obtain the highest quality, long DNA it is important to start with sample types compatible with HMW DNA extraction methods. This technical note is intended to give general guidance on sample collection, preparation, and storage across a range of commonly encountered sample types used for SMRT Sequencing whole genome projects. It is important to note that all samples and projects are unique and may not be comprehensively addressed in this document.
Technical note — Preparing DNA for PacBio HiFi sequencing – Extraction and quality control
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA in order to produce highly accurate long reads, or HiFi reads. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in longer libraries and better performance during sequencing. This technical note is intended to give recommendations, tips and tricks for the extraction of DNA, as well as assessing and preserving the quality and size of your DNA sample to be used for HiFi sequencing.