Discoveries Roadshow
After two years apart, we’ve missed you, so we’re coming to a city near you. We are visiting 20 cities across North America and Europe during April and May. Join us…
After two years apart, we’ve missed you, so we’re coming to a city near you. We are visiting 20 cities across North America and Europe during April and May. Join us…
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
PacBio Vice President of Segment Marketing, Dr. Jennifer Stone, demonstrates how HiFi sequencing is changing the game in human genetics by sharing some of the exciting milestones and seminal publications…
The Environmental and Agronomical Genomics 2021 symposium is jointly organised by France Génomique (FG) and the GDR Génomique Environnementale (GE). The Environmental and Agronomical Genomics 2021 symposium will be the opportunity to…
Understanding genome sequences and how they evolved is critical for harnessing that evolutionary process for agricultural improvement. Whether asking questions about the gain/loss of genes, the role of structural variation…
Join us for this webinar to understand the evolution of sequencing technologies and where they stand today, the experiences of three plant biologists incorporating sequencing into their work, and how…
Korean service provider DNA Link has established strong expertise with the PacBio sequencing platform in response to high global demand for the technology.
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in better sequencing performance across difficult to sequence regions of the genome. To obtain the highest quality, long DNA it is important to start with sample types compatible with HMW DNA extraction methods. This technical note is intended to give general guidance on sample collection, preparation, and storage across a range of commonly encountered sample types used for SMRT Sequencing whole genome projects. It is important to note that all samples and projects are unique and may not be comprehensively addressed in this document.
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA in order to produce highly accurate long reads, or HiFi reads. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in longer libraries and better performance during sequencing. This technical note is intended to give recommendations, tips and tricks for the extraction of DNA, as well as assessing and preserving the quality and size of your DNA sample to be used for HiFi sequencing.
Learn how highly accurate long-read sequencing from the Sequel IIe Systems delivers data you can trust for advanced biological insights across a range of applications.
With SMRT Link you can unlock the power of PacBio Single Molecule, Real-Time (SMRT) Sequencing using our portfolio of software tools designed to set up and monitor sequencing runs, review performance metrics, analyze, visualize, and annotate your sequencing data.
The SMRTbell Express Template Prep Kit 2.0 provides a streamlined, single-tube reaction strategy to generate SMRTbell libraries from 500 bp to >50 kb insert size targets to support large-insert genomic libraries, multiplexed microbial genomes and amplicon sequencing. With this new formulation, we have increased both the yield and efficiency of SMRTbell library preparation for SMRT Sequencing while further minimizing handling-induced DNA damage to retain the integrity of genomic DNA (gDNA). This product note highlights the key benefits, performance, and resources available for supporting de novo genome sequencing and structural variant detection projects. Our large-insert gDNA protocol has been streamlined to support SMRTbell library generation in only 4 hours, making complete construction in less than a day. This significantly reduces time to results for generating high-quality genome assemblies to fully characterize SNPs and structural variants. Additional key benefits of this template preparation kit and updated protocol include library generation with as little as 2 to 3 µg input gDNA and flexibility to accommodate and adjust input amount in accordance with the extracted gDNA quality.
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