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August 19, 2021  |  Product and Application Notes

Product note — SMRTbell express template prep 2.0 for large-insert libraries

The SMRTbell Express Template Prep Kit 2.0 provides a streamlined, single-tube reaction strategy to generate SMRTbell libraries from 500 bp to >50 kb insert size targets to support large-insert genomic libraries, multiplexed microbial genomes and amplicon sequencing. With this new formulation, we have increased both the yield and efficiency of SMRTbell library preparation for SMRT Sequencing while further minimizing handling-induced DNA damage to retain the integrity of genomic DNA (gDNA). This product note highlights the key benefits, performance, and resources available for supporting de novo genome sequencing and structural variant detection projects. Our large-insert gDNA protocol has been streamlined to support SMRTbell library generation in only 4 hours, making complete construction in less than a day. This significantly reduces time to results for generating high-quality genome assemblies to fully characterize SNPs and structural variants. Additional key benefits of this template preparation kit and updated protocol include library generation with as little as 2 to 3 µg input gDNA and flexibility to accommodate and adjust input amount in accordance with the extracted gDNA quality.

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