Product Line: Sequel II system and Sequel IIe system
May 6, 2024
Does PacBio have a fully automated workflow solution?
PacBio has fully automated workflows for DNA extraction, DNA shearing, whole genome library prep, and targeted workflows.
May 6, 2024
Which library prep kit should I use for preparing libraries for Microbial WGS on HiFi systems?
HiFi plex prep kit 96. This kit and protocol offer the most cost efficient and streamlined prep for doing microbial WGS on HiFi systems.
May 6, 2024
Which library prep kit should I use for human, plant, or animal WGS on HiFi sequencing systems?
24 reaction kit: SMRTbell prep kit 3.0 (102-182-700) 96 reaction kit: HiFi prep kit 96 (103-381-200) Both kits and protocols use the same enzymatic steps and have qualified automated methods…
April 24, 2024
For PureTarget, can I use samples that are not human blood and cell line extracted with Nanobind?
The supported sample types for PureTarget are Nanobind-extracted blood, cell line, and saliva. Please see table 4 in the app note (https://www.pacb.com/wp-content/uploads/Application-note-Comprehensive-genotyping-with-the-PureTarget-repeat-expansion-panel-and-HiFi-sequencing.pdf) for more details about other extraction kits and…
April 24, 2024
What guidance do you have for adding a small number of guide RNA pairs to the fixed PureTarget panel?
Please see the Tech note on custom PureTarget panels for more details.
April 24, 2024
Can I do a custom PureTarget panel?
Yes. Please see the Tech note on custom PureTarget panels for more details.
April 11, 2024
Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system
April 11, 2024
Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system
April 11, 2024
Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system
April 11, 2024