This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented.
This procedure describes the workflow for constructing single-cell MAS-Seq (Multiplexed Arrays Sequencing) libraries from 10x Chromium 3’ cDNA using the MAS-Seq for 10x Single Cell 3’ kit for library prep and sequencing on PacBio systems.
This document describes how to use PacBio’s SMRT Link software (v11.1). SMRT Link is the web-based end-to-end workflow manager for Sequel II/IIe systems.
Product insert for MAS-Seq for 10x single cell 3′ concatenation kit (PN 102-407-900)
Safety data sheet for MAS-Seq for 10x Single Cell 3′ kit (PN 102-691-900)
Release notes for SMRT Link v11.1.
This document describes the procedure for installing SMRT Link v11.1 on a customer system. This document is for use by Customer IT.
The MAS capture beads kit has streptavidin beads which can be used in combination with a biotynlated probe. It is intended to be used to capture the 3′ end of 10x cDNA molecules.
This document describes the command-line tools included with SMRT Link v11.1. These tools are used by bioinformaticians working with secondary analysis results.
Safety data sheet for MAS capture beads kit (PN 102-692-000)