This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Back in April we introduced significant software enhancements to simultaneously provide the base sequence and 5-methylcytosine DNA methylation status by default, making the Sequel II/IIe systems the world’s first (and…
This week Illumina gave an update on their synthetic long-read approach previously named “Infinity reads,” now rebranded as Complete Long Reads (CLR). This acronym choice is of course quite ironic…
Over the past few months, we have highlighted the striking versatility of HiFi sequencing in a series of a dozen blogposts, allowing many different research applications to be performed with…
In living cells, it is typically the proteins that are the work horses of carrying out the myriad of biological functions, and that can be subject to aberrant processes that…
In a new preprint, entitled Realizing the promise of biodiversity genomics with highly accurate long reads, a group of researchers from Utah State, BYU, the LOEWE Centre for Translational Biodiversity…
The combined accuracy, read length, and coverage uniformity of PacBio HiFi sequence reads enables the characterization of many previously inaccessible regions in the genome. Two new preprints by Oscar Rodriguez,…
A few weeks ago, we described the use of HiFi sequencing to drive the paradigm shift from a single reference genome to a pangenome derived from a diverse set of…
More precise data provided by PacBio HiFi sequencing can have a marked impact on the resolving power in genetic association studies and decrease the required cohort sizes. As a recent…
PacBio HiFi sequencing for the de novo genome assembly of microbial, fungal, plant, animal and human genomes has been described in hundreds of publications, and its best-in-class performance has been…
The combined accuracy and length of PacBio HiFi reads is utilized by an ever-increasing number of researchers, with numerous new publications and preprints appearing every week. Several months ago, Illumina…
Most higher organisms are diploid, meaning that each cell carries in its nucleus two copies of the individual’s genome, one from its mother and one from its father. Accurately separating…
The scientific community is still abuzz from last week’s announcement of the amazing achievement of the first complete sequence of a human genome by the Telomere-to-Telomere (T2T) Consortium. Assembling genomes…
Through the development of long-read, full-length RNA sequencing methods, the scientific community has recognized a fundamental paradigm shift, “from ‘gene-centric’ to ‘isoform-centric’ understanding of human transcriptomes, owing to the frequent…
Long-read PacBio HiFi sequencing has made transformative contributions to many areas of genomics over the past few years, including de novo assembly, calling of all variant types, full-length phased targeted…
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