Asset Tag: de novo assembly
Application brief — Microbial whole genome sequencing
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.
Procedure & Checklist — Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0
Procedure & Checklist — Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0
With Long Reads and Short Reads, the Possibilities are Endless – PacBio ASHG 2021 Fireside Chat
With the September 2021 closing of PacBio’s acquisition of Omniome, PacBio intends to become the first company to offer both long-read and short-read sequencing platforms. What does this mean for…
Four Chromosome Scale Genomes and a Pan-Genome Annotation to Accelerate Pecan Tree Breeding
In this SMRT Science Journal Club talk, John Lovell from HudsonAlpha Institute for Biotechnology discusses his work constructing and analyzing de novo pecan genome assemblies and annotations to help accelerate…
Getting the Most Out of Your Breeding Program with DNA and RNA Sequencing
Understanding genome sequences and how they evolved is critical for harnessing that evolutionary process for agricultural improvement. Whether asking questions about the gain/loss of genes, the role of structural variation…
Long-Read Genome Sequencing for the Molecular Understanding of Neurodevelopmental Disorders
In this ESHG 2021 Workshop, PacBio Chief Scientific Officer Jonas Korlach, Ph.D., describes why HiFi sequencing improves the ability to detect pathogenic variants that previously went undetected with other technologies. He…
Procedure & Checklist — Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0
Certified Service Provider Profile: In Seoul, DNA Link offers excellence in SMRT Sequencing
Korean service provider DNA Link has established strong expertise with the PacBio sequencing platform in response to high global demand for the technology.
Technical note: Preparing samples for PacBio whole genome sequencing for de novo assembly – Collection and storage
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in better sequencing performance across difficult to sequence regions of the genome. To obtain the highest quality, long DNA it is important to start with sample types compatible with HMW DNA extraction methods. This technical note is intended to give general guidance on sample collection, preparation, and storage across a range of commonly encountered sample types used for SMRT Sequencing whole genome projects. It is important to note that all samples and projects are unique and may not be comprehensively addressed in this document.
Technical note — Preparing DNA for PacBio HiFi sequencing – Extraction and quality control
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA in order to produce highly accurate long reads, or HiFi reads. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in longer libraries and better performance during sequencing. This technical note is intended to give recommendations, tips and tricks for the extraction of DNA, as well as assessing and preserving the quality and size of your DNA sample to be used for HiFi sequencing.
Brochure — SMRT Link: Explore and analyze your data with confidence
With SMRT Link you can unlock the power of PacBio Single Molecule, Real-Time (SMRT) Sequencing using our portfolio of software tools designed to set up and monitor sequencing runs, review performance metrics, analyze, visualize, and annotate your sequencing data.