Workflow Step: Sample preparation
Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the KingFisher Apex system
Procedure & checklist — Extracting-HMW DNA from plant nuclei using the Nanobind PanDNA kit
Technical overview — Automated high-throughput HMW DNA extraction for PacBio long-read sequencing using Nanobind HT kits
Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system
Procedure & checklist — Extracting DNA from cultured suspension cells using Nanobind kits
Technical overview — HMW DNA sample preparation for PacBio long-read sequencing using Nanobind PanDNA and SRE kits
Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing
Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing
What are my options for barcoding samples?
You can find all the resources for barcoding all types of samples on the multiplexing page
Can I get methylation data from amplified DNA?
No. DNA amplification removes base modifications, so methylation information is lost. To detect methylation accurately, use native DNA, which preserves base modifications for analysis
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…
How much DNA or cDNA do I need to do HiFi sequencing?
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…