Workflow Step: Library preparation
August 1, 2024
How can I use HiFi bioinformatics to uncover germline variation?
Watch our video on HiFi sequencing bioinformatics for uncovering germline variation. It explains how HiFi reads provide a more complete view of the genome, introduces SMRTLink and open-source GitHub tools…
August 1, 2024
Why would you need long reads for RNA sequencing and how do the Kinnex kits work?
Watch our educational video to learn the why, what, and how of PacBio Kinnex kits. The video explains why long reads are valuable for RNA sequencing, what the Kinnex kits…
July 12, 2024
Where can I learn about the extraction of high molecular weight DNA, which is optimal for long-read sequencing?
Watch our video, Extraction to Revio System, We’ve got you covered, to learn about best practices for extracting high molecular weight DNA for long-read sequencing. The video highlights the strong…
May 6, 2024
Does PacBio have a fully automated workflow solution?
PacBio has fully automated workflows for DNA extraction, DNA shearing, whole genome library prep, and targeted workflows.
May 6, 2024
Which library prep kit should I use for preparing libraries for Microbial WGS on HiFi systems?
HiFi plex prep kit 96. This kit and protocol offer the most cost efficient and streamlined prep for doing microbial WGS on HiFi systems.
May 6, 2024
Which library prep kit should I use for human, plant, or animal WGS on HiFi sequencing systems?
24 reaction kit: SMRTbell prep kit 3.0 (102-182-700) 96 reaction kit: HiFi prep kit 96 (103-381-200) Both kits and protocols use the same enzymatic steps and have qualified automated methods…
April 24, 2024
For PureTarget, can I use samples that are not human blood and cell line extracted with Nanobind?
The supported sample types for PureTarget are Nanobind-extracted blood, cell line, and saliva. Please see table 4 in the app note (https://www.pacb.com/wp-content/uploads/Application-note-Comprehensive-genotyping-with-the-PureTarget-repeat-expansion-panel-and-HiFi-sequencing.pdf) for more details about other extraction kits and…
April 24, 2024
What guidance do you have for adding a small number of guide RNA pairs to the fixed PureTarget panel?
Please see the Tech note on custom PureTarget panels for more details.
April 24, 2024
Can I do a custom PureTarget panel?
Yes. Please see the Tech note on custom PureTarget panels for more details.
April 1, 2024
Technical overview — HiFi library preparation using HiFi prep kits for high-throughput sequencing on PacBio long-read systems
March 25, 2024