HiFi reads are produced using circular consensus sequencing (CCS) mode on PacBio long-read systems. HiFi reads provide base-level resolution with 99.9% single-molecule read accuracy. HiFi reads can be used across…
Some short-read analysis tools can be used with long-read data, especially if they include options optimized for longer read lengths. However, most short-read tools are built around assumptions that differ…
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
Yes, we offer PureTarget panels for PCR free targeted sequencing, hybrid capture workflows with our partners, and amplicon sequencing. Visit the targeted sequencing page for further information.
No purchase is required. PacBio software is free and available from our Software & Bioinformatics webpage, Bioconda, DockerHub, http://Quay.io , and the PacBio GitHub. Depending on your analysis needs, you…
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…
If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.