December 18, 2023
Product Line: Sequel II system and Sequel IIe system
December 14, 2023
Do my primers need to be 5′ phosphorylated for amplicon sequencing?
No. Your primers do not need to be 5′ phosphorylated for amplicon sequencing.
December 14, 2023
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices.
December 14, 2023
What are my options for barcoding samples?
You can find all the resources for barcoding all types of samples on the multiplexing page
December 13, 2023
Which protocol do I need to follow to multiplex microbial libraries for WGS?
You can use the HiFi plex prep kit 96 and this protocol for multiplex microbial WGS and shotgun metagenomics applications
December 13, 2023
Can I multiplex more than 384 amplicon samples?
Yes. With PacBio barcodes, you can create thousands of asymmetrical combinations for high-throughput multiplexing. While not all combinations have been formally validated, many customers have successfully implemented expanded multiplexing strategies….
December 13, 2023
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…
December 13, 2023
Where can I find the most recent protocols for long-read sequencing?
You can find the protocols (procedure & checklists) on the protocols page (filtered view from featured documentation).
December 13, 2023
How do I import a dataset into SMRTLink if automated import fails?
Go to Data Management and click “Import Data” In the Data Type drop-down select “HiFi Reads or Subreads (XML)” In the file browser below, navigate to the directory to which the…
December 13, 2023
Why do I get an error [Missing Reference Annotation] in SMRT Link run design when setting up a run?
Before running applications that require reference annotation (selecting Kinnex in run design) for the first time, a reference annotation must be added to SMRT Link or an error is thrown…
December 13, 2023
What analysis tools are available?
PacBio provides a wide range of analysis options to support your research. SMRT Link includes built-in analysis pipelines for many common applications, offering an easy-to-use, integrated environment. For advanced or…
December 13, 2023
What to do if demultiplexing is not done correctly or the results are not as expected?
If the wrong barcodes were selected, use one of the two options: SMRT Link: use the Data Utility “Undo Demultiplexing” (make sure to run on the parent dataset) and then…