This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Last updated: 30 MAR 2026 Long-read sequencing technologies such as PacBio HiFi sequencing are quickly becoming the new gold standard in genomics research. This article provides an introductory look at…
A new preprint entitled Utility of long-read sequencing for All of Us – from research groups at Baylor College of Medicine, the Broad Institute, Jackson Laboratory, Discovery Life Sciences, Harvard…
Back in April we introduced significant software enhancements to simultaneously provide the base sequence and 5-methylcytosine DNA methylation status by default, making the Sequel II/IIe systems the world’s first (and…
This week Illumina gave an update on their synthetic long-read approach previously named “Infinity reads,” now rebranded as Complete Long Reads (CLR). This acronym choice is of course quite ironic…
PacBio is pleased to present the recipients of this year’s HiFi for accuracy SMRT grant. At PacBio, we are driven to create the most accurate genomic sequencing technologies so that you…
Over the past few months, we have highlighted the striking versatility of HiFi sequencing in a series of a dozen blogposts, allowing many different research applications to be performed with…
In living cells, it is typically the proteins that are the work horses of carrying out the myriad of biological functions, and that can be subject to aberrant processes that…
The combined accuracy, read length, and coverage uniformity of PacBio HiFi sequence reads enables the characterization of many previously inaccessible regions in the genome. Two new preprints by Oscar Rodriguez,…
A few weeks ago, we described the use of HiFi sequencing to drive the paradigm shift from a single reference genome to a pangenome derived from a diverse set of…
PacBio HiFi sequencing has been utilized to generate reference-quality genomes in many studies, most notably in finally completing a human genome. Following this work, the Human Pangenome Reference Consortium…
PacBio HiFi sequencing for the de novo genome assembly of microbial, fungal, plant, animal and human genomes has been described in hundreds of publications, and its best-in-class performance has been…
The scientific community is still abuzz from last week’s announcement of the amazing achievement of the first complete sequence of a human genome by the Telomere-to-Telomere (T2T) Consortium. Assembling genomes…
What would be possible if every human genetics researcher had a complete reference genome? Today, researchers use a good – but incomplete – reference genome released in 2013. As…
Single Molecule, Real-Time (SMRT) Sequencing continues to get smarter and more powerful, with the recent launch of the Sequel II system increasing capabilities and efficiencies of the long-read DNA and…
When looking to understand the functional implications of genetic variability, scientists should seek out the Iso-Seq method, according to Cold Spring Harbor researchers. In a recent paper published in Frontiers…
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