In this SMRT Science Journal Club talk, Mikhail Kolmogorov from the University of California Santa Cruz discusses his computational approach to the generation of lineage-resolved complete MAGs by precision phasing.
In this SMRT Science Journal Club talk, Phillip Tai from the University of Massachusetts Medical School discusses his investigation in the design compatibility of CRISPR components in AAV vectors.
Understanding genome sequences and how they evolved is critical for harnessing that evolutionary process for agricultural improvement. Whether asking questions about the gain/loss of genes, the role of structural variation on phenotypic diversity, or identifying favorable alleles in exotic and wild species, DNA and RNA sequencing have proven to be extremely valuable tools for breeding programs all over the world. This panel-style webinar brings together three plant biologists to talk about their journeys into sequencing plant genomes and how these efforts have helped push plant breeding forward.
In this ESHG 2021 Workshop, PacBio Chief Scientific Officer Jonas Korlach, Ph.D., describes why HiFi sequencing improves the ability to detect pathogenic variants that previously went undetected with other technologies. He then turns the microphone over to Susan Hiatt, Ph.D. from HudsonAlpha Institute for Biotechnology. Dr. Hiatt discusses how she and her team used HiFi sequencing in their rare disease research to discover genomic variation missed by whole-exome or genome sequencing studies using short reads, allowing her team to uncover medical mysteries that had previously gone unexplained.
In this talk, speakers will describe the importance of high accuracy and long read length for generating closed bacterial assemblies. Speakers will also share examples of how hard-to-assemble domains and plasmids impact important biological traits including, pathogen virulence and anti-microbial resistance. Finally, they will provide an overview of the advantages of highly accurate long-read sequencing for outbreak tracking.
In this talk, speakers provide an understanding of how highly accurate long-read sequencing of extended 16S amplicons enables the identification of metagenome community members at higher taxonomic resolution than short-read methods. You’ll also hear examples of how metagenome functions that impact human health can be driven by specific species or strains within a community and learn how the gut microbiome can impact drug efficacy.
In this video Shawn Levy, Discovery Life Sciences’ Chief Scientific Officer, along with Cheryl Heiner, PacBio Principal Scientist, discuss the advantages of HudsonAlpha Discovery’s specialized sequencing services for PacBio HiFi reads to advance research for clinical, translational, and other studies spanning a range of diseases.
In this talk, speakers provide an understanding HiFi sequencing methods for resolving viral diversity in complex systems, examples of how HiFi sequencing can phase entire viral genes or genomes, revealing quasispecies diversity within patients, and how combining fully-phased minor variant data with other data types provides insights into viral evolution, immune escape, and drug resistance.
In this talk, speakers provide an overview of PacBio-recommended tools for metagenome sequencing analysis, where to download example test data, the typical performance for HiFi metagenome sequencing of fecal samples, the impact of read accuracy on metagenome assembly of long-read data, and finally, how deep sequencing that combines HiFi reads and Hi-C data can enormously increase recovery of high-quality MAGs and connect plasmids and viruses to host strains.
In this SMRT Science Journal Club talk, Anoushka Joglekar from Weill Cornell Medicine discusses how she and her colleagues are developing tools to produce an isoform view of the brain in order to better understand developmental disorders and neurodegenerative disease.
In this SMRT Science Journal Club talk, Phillip Tai from the University of Massachusetts Medical School discusses his investigation in the design compatibility of CRISPR components in AAV vectors.
Through Pharmacogenomics (PGx), we can explore how a person’s genome affects their response to drugs to enable the development of safe and effective medications tailored to their genetic makeup. In this talk, you’ll learn how PacBio HiFi sequencing: is cost-effective and highly accurate; enables comprehensive interrogation of pharmacogenomics genes—detecting all types of variants even in challenging regions; allows for the sequencing of pharmacogenomics genes as single-gene assays or large panels; produces data that is highly concordant with other technologies—adding value through comprehensive variant detection, copy number assessment, and phasing.
Join PacBio’s Mike Weiand to hear how gene editing protocols can benefit from long-read sequencing at multiple stages to optimize and enhance vector integrity. This method allows researchers to reveal findings that other sequencing methods can not identify due to read-length limitations or amplification biases.
The Sequel IIe System produces highly accurate long reads (HiFi reads) quickly and cost-effectively thanks to increased computational capacity and on-instrument data processing. This results in a significant reduction of overall secondary analysis time and reduces the computational needs in terms of file transfer and data storage. This webinar provides an overview of PacBio data for beginner and advanced users of HiFi reads, exploring the content of PacBio BAM files, providing examples of how HiFi reads can be filtered, and going through recommendations for downstream analysis.
With highly accurate long reads (HiFi reads) of >99% accuracy, the Sequel IIe System is the tool of choice for producing reference quality assemblies, calling variants with the highest precision, reading full-length transcripts, characterizing isoform diversity, and many more applications. To maximize the quality of your results, DNA and sample preparation are critical steps in your sequencing workflow. In this webinar we cover the following points: preparing DNA for PacBio HiFi sequencing, selecting the right HiFi library preparation protocol for your project, and interpreting sequencing metrics and troubleshooting.